AUTHOR=Zhong Feng , Liu Lei , Wei Jia-Li , Dai Ru-Ping 

TITLE=Step by Step Golgi-Cox Staining for Cryosection

JOURNAL=Frontiers in Neuroanatomy

VOLUME=Volume 13 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/neuroanatomy/articles/10.3389/fnana.2019.00062

DOI=10.3389/fnana.2019.00062

ISSN=1662-5129

ABSTRACT=Golgi staining, though invented hundreds of years ago, is still a reliable method to study cytoarchitecture of the brain. Almost all published Golgi staining protocols and methods were for microtome, and it was rarely applied in cryosection, which restricted the application of this technique. Currently, several commercial Golgi-stain kits are available for both:  vibratome section and cryosection, but these kits are costly and it is still challenging for researchers to get fine results. In the present study, we described a protocol of Golgi-Cox Staining for Cryosection, with the modified cryosection protection solution based on the Golgi –Cox method, which makes cryosection easily applied  in the section of the Golgi-Cox impregnated tissue. Our methods provide a low-cost and simple option for Golgi staining, and it will facilitate researchers to get fine Golgi staining results for neuronal architecture studies.