AUTHOR=Stein Jason , Xu Quangang , Jackson Kayla C. , Romm Elena , Wuest Simone C. , Kosa Peter , Wu Tianxia , Bielekova Bibiana 

TITLE=Intrathecal B Cells in MS Have Significantly Greater Lymphangiogenic Potential Compared to B Cells Derived From Non-MS Subjects

JOURNAL=Frontiers in Neurology

VOLUME=Volume 9 - 2018

YEAR=2018

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2018.00554

DOI=10.3389/fneur.2018.00554

ISSN=1664-2295

ABSTRACT=Although B cell depletion is an effective therapy of multiple sclerosis (MS), the pathogenic functions of B cells in MS remain incompletely understood. We asked whether cerebrospinal fluid (CSF) B cells in MS secrete different cytokines than control-subject B cells and whether cytokine secretion affects MS phenotype. We blindly studied CSF B cells after their immortalization by Epstein-Barr Virus (EBV) in prospectively-collected MS patients and control subjects with other inflammatory-(OIND) or non-inflammatory neurological diseases (NIND) and healthy volunteers (HV). The pilot cohort was analyzed using intracellular cytokine staining (n=101 B cell lines [BCL] derived from 80 subjects). We validated differences in cytokine production in newly-generated CSF BCL (n=207 derived from subsequent 111 prospectively-recruited subjects representing validation cohort), using ELISA enhanced by objective, flow-cytometry-based B cell counting. After unblinding the pilot cohort, the immortalization efficiency was almost 5 times higher in MS patients compared to controls (p<0.001). MS subjects’ BCLs produced significantly more vascular endothelial growth factor (VEGF) compared to control BCLs. Progressive MS patients BCLs produced significantly more tumor necrosis factor (TNF)-α and lymphotoxin (LT)-α than BCL from relapsing-remitting MS (RRMS) patients.  In the validation cohort, we observed lower secretion of IL-10, but also IL-1b in RRMS patients, compared to HV+NIND cohort. The validation cohort validated enhanced VEGF-C production by BCL from RRMS patients and higher TNF-α and LT-α secretion by BCL from progressive MS. No significant differences among diagnostic categories were observed in secretion of IL-6 or GM-CSF. All three cytokines with increased secretion in different stages of MS (i.e. VEGF-C, TNF-α and LT-α enhance lymphangiogenesis, suggesting that intrathecal B cells directly facilitate the formation of tertiary lymphoid follicles, thus compartmentalizing inflammation to the central nervous system.