AUTHOR=Zhao Hongye , Zheng Tiezheng , Yang Xiaohan , Fan Ming , Zhu Lingling , Liu Shuhong , Wu Liying , Sun Changkai 

TITLE=Cryptotanshinone Attenuates Oxygen-Glucose Deprivation/ Recovery-Induced Injury in an in vitro Model of Neurovascular Unit

JOURNAL=Frontiers in Neurology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2019.00381

DOI=10.3389/fneur.2019.00381

ISSN=1664-2295

ABSTRACT=Cryptotanshinone (CTs), an active component isolated from the root of Salvia miltiorrhiza, has been demonstrated to be effective for both myocardial and hepatic ischemia/reperfusion damage. We here established an oxygen-glucose deprivation/recovery (OGD/R) model of Neurovascular Unit (NVU) in vitro to observe the effects of CTs on cerebral ischemia/reperfusion injury (CIRI), and explore the underlying mechanisms. CTs was observed to significantly inhibit the OGD/R-induced neuronal apoptosis, and reduce the activation of Caspase-3 and poly (ADP-ribose) polymerase (PARP), as well as the increase of Bax/Bcl-2 ratio in neurons under OGD/R condition. The inhibitory effects of CTs on neuron apoptosis were associated with the blocking of the JNK and p38 MAPK signaling pathway. CTs also remarkably ameliorated OGD/R-induced reduction of transepithelial membrane resistance (TEER) values and the increase of transendothelial permeability coefficient (Pe) of sodium fluorescein (SF) by enhancing the expression of ZO-1, Occludin and Claudin-5 in BMECs, which might be related to the downregulation of matrix metalloprotein (MMP)-9 expression. Altogether, our data indicate that CTs has protective effects against OGD/R injure in NVU models in vitro via the amelioration of neuronal apoptosis and BBB disrupt, suggesting that CTs is a candidate medicine for CIRI.