AUTHOR=Gabriel-Salazar Marina , Lei Ting , Grayston Alba , Costa Carme , Medina-Gutiérrez Esperanza , Comabella Manuel , Montaner Joan , Rosell Anna 

TITLE=Angiogenin in the Neurogenic Subventricular Zone After Stroke

JOURNAL=Frontiers in Neurology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.662235

DOI=10.3389/fneur.2021.662235

ISSN=1664-2295

ABSTRACT=Ischemic stroke is a leading cause of death and disability worldwide with effective acute thrombolytic treatments. However brain repair mechanisms related to spontaneous or rehabilitation-induced recovery are still under investigation and little is known about the molecules involved. The present study examines the potential role of angiogenin (ANG), a known regulator of cell function and metabolism linked to neurological disorders, focusing in the neurogenic subventricular zone (SVZ). Angiogenin expression was examined in the mouse SVZ, in SVZ-derived neural stem cells (NSCs) which were exposed to exogenous ANG treatment during neurosphere formation as well as other neuron-like cells (SH-SY5Y). Additionally, male C57Bl/6 mice underwent a dital permanent occlusion of the middle cerebral artery to study endogenous and exercise-induced expression of SVZ-ANG and neuroblast migration. Our results show that SVZ areas are rich in ANG, primarily expressed in DCX+ neuroblasts but not in Nestin+ Neural Stem Cells (NSCs). In vitro, treatment with ANG increased the number of SVZ-derived NSCs forming neurospheres, but could not modify SH-SY5Y neurite differentiation. Finally, physical exercise rapidly increased the amount of endogenous ANG in the ipsilateral SVZ niche after ischemia, where DCX migrating cells increased as part of the post-stroke neurogenesis process. Our findings position for the first time ANG in the SVZ during post-stroke recovery which could be linked to neurogenesis.