AUTHOR=Jiang Xiaoming , Lu Yongyu , Hu Xiaogang , She Xiaochun 

TITLE=Dissolution Effect of Alteplase on Arterial Blood Clot Model of Hypertensive Intracerebral Hemorrhage Patients in vitro

JOURNAL=Frontiers in Neurology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2022.729727

DOI=10.3389/fneur.2022.729727

ISSN=1664-2295

ABSTRACT=Abstract: Objective: To explore the dissolution effect of alteplase (rt-PA) on arterial blood clots of patients with hypertensive cerebral hemorrhage in vitro and analyze the optimal concentration and action time of rt-PA for intracranial hematomas. Methods: The arterial blood of 35 patients with confirmed hypertensive cerebral hemorrhage were collected, centrifuged, and the serum was aspirated to prepare the blood clot model. The 0.125 mg, 0.25 mg, 0.5 mg, 1 mg, 2 mg, 3 mg t-PA, 20,000 U, and 40,000 U urokinase (u-PA) were taken for the corresponding blood clot for dissolution test. The blood clot volume and dissolution volume were measured at 0 min, 30 min, 60 min, 90 min, 120 min, and 150 min. Results: Without intervention, the blood clot volume of men were higher than that of women at 0 min, 30 min, 60 min, and 90 min (P＜0.05). Without intervention, hematocrit (HCT) was correlated with blood clot volume and the correlation decreased with time. The 30 min, 60 min, and 90 min dissolution curves of each group showed an upward trend (P<0.05), and the dissolution curves tended to be flat at 120 min and 150 min. The dissolution volume of 0.125 mg/3ml, 0.25 mg/3ml, 0.5 mg/3ml rt-PA, 20000 U, 40000 U u-PA was higher than that of 1 mg/ml, 2 mg/ml, 3 mg/ml rt-PA (P＜0.05). The dissolution volume of 0.125 mg/3ml, 0.25 mg/3ml, 0.5 mg/3ml rt-PA was not significantly different from 20000 U and 40000 U u-PA (P>0.05). Gender differences had no effect on the effects of the above drugs. Conclusion: In vitro, low-concentration rt-PA has a better dissolution effect, and it shows a time-dependent effect, reaching the highest effect in 90 minutes.
Key words: alteplase; urokinase; hypertensive cerebral hemorrhage; arterial blood; Dissolution