AUTHOR=Tang Yuyuan , Wu Jun'e , Xu Zhirui , Fan Baochao , Li Xiangli , Jin Bingxu , Tang Chunzhi 

TITLE=Mechanism of Qihuang needle therapy in the management of tic disorders: a clinical trial protocol

JOURNAL=Frontiers in Neurology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2023.1036453

DOI=10.3389/fneur.2023.1036453

ISSN=1664-2295

ABSTRACT=<sec><title>Background</title><p>Qihuang needle therapy is a newly developed acupuncture therapy to treat tic disorders in clinical practice. However, the mechanism to reduce tic severity remains unknown. Changes in intestinal flora and circulation metabolites are perhaps the potential pathogenesis of tic disorders. As a result, we present a protocol for a controlled clinical trial using multi-omics analysis to probe the mechanism of the Qihuang needle in managing tic disorders.</p></sec><sec><title>Methods</title><p>This is a matched-pairs design, controlled, clinical trial for patients with tic disorders. Participants will be allocated to either an experimental group or a healthy control group. The main acupoints are Baihui (GV20), Yintang (EX-HN3), and Jueyinshu (BL14). The experimental group will receive Qihuang needle therapy for a month, while the control group will receive no interventions.</p></sec><sec><title>Expected outcomes</title><p>The change in the severity of the tic disorder is set as the main outcome. Secondary outcomes include gastrointestinal severity index and recurrence rate, which will be calculated after a 12-week follow-up. Gut microbiota, measured by 16S rRNA gene sequencing; serum metabolomics, assessed <italic>via</italic> LC/MS; and serum zonulin, assessed by enzyme-linked immunosorbent assay (ELISA), will be used as biological specimen analysis outcomes. The present study will investigate the possible interactions between intestinal flora and serum metabolites and the improvement of clinical profiles, which may elucidate the mechanism of Qihuang needle therapy for tic disorders.</p></sec><sec><title>Trial registration</title><p>This trial is registered at the Chinese Clinical Trial Registry (<ext-link ext-link-type="uri" xlink:href="http://www.chictr.org.cn/" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.chictr.org.cn/</ext-link>). Registration number: ChiCTR2200057723, Date: 2022-04-14.</p></sec>