AUTHOR=Zhao Liang , Li Pingping , Xu Ziao , Ji Xuefei , Guan Liao , Wang Xiaojian , Luo Jin , Cheng Hongwei , Ye Lei TITLE=Diagnosis of post-neurosurgical bacterial meningitis in patients with aneurysmal subarachnoid hemorrhage based on the immunity-related proteomics signature of the cerebrospinal fluid JOURNAL=Frontiers in Neurology VOLUME=Volume 14 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2023.1166598 DOI=10.3389/fneur.2023.1166598 ISSN=1664-2295 ABSTRACT=Post-neurosurgical bacterial meningitis (PNBM) is a serious complication for patients receiving neurosurgical treatment, but the diagnosis is difficult given the complicated microenvironment orchestrated by sterile brain injury and pathogenic infection. In this study, we explore the potential diagnostic biomarkers and immunological features by using a proteomics platform. Thirty-one patients with aneurysmal subarachnoid hemorrhage (aSAH) who received neurosurgical treatment were recruited in this study. Among them, 15 were diagnosed with PNBM. The remaining 16 patents were categorized into the non-PNBM group. Proteomics analysis of the cerebrospinal fluid (CSF) was performed by Olink platform that contained 92 immunity-related molecules. We found that the expressions of 27 CSF proteins were significantly different between the PNBM and non-PNBM groups. Of those, 15 proteins were up-regulated and 12 were down-regulated in the CSF of the PNBM group. Receiver operating characteristic curve analysis indicated that three proteins (PTN, CD27, and ANGPT1) had high diagnostic accuracy for PNBM. Furthermore, we also performed bioinformatics analysis to explore the potential pathways and subcellular localization of the proteins. In summary, we found a cohort of immunity-related molecules that can serve as potential diagnostic biomarkers for PNBM in the patients with aSAH. These molecules also provide an immunological profile of PNBM.