Front. Neurol.Frontiers in NeurologyFront. Neurol.1664-2295Frontiers Media S.A.10.3389/fneur.2023.1180873NeurologyOriginal ResearchRETRACTED: Deficits in the thalamocortical pathway associated with hypersensitivity to pain in patients with frozen shoulderLiTengshuai1†LiJie1†ZhaoRui2†ZhouJiaming2*ChuXu3*1Department of Orthopedic Surgery, Tianjin Hospital, Tianjin, China2Department of Orthopedic Surgery, Tianjin Medical University General Hospital, Tianjin, China3Department of Orthopaedics, Honghui Hospital, Xi'an Jiaotong University, Xian, China
Edited by: Arpad Pardutz, University Szeged, Hungary
Reviewed by: Jian Jiang, The First Affiliated Hospital of Nanchang University, China; Denggui Fan, University of Science and Technology Beijing, China; Nikoletta Szabó, University of Szeged, Hungary
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Background and purpose
Frozen shoulder (FS) is a chronic pain condition and has been shown to be associated with pain sensitization. However, the underyling brain mechanisms remain unclear. Here, we aimed to explore brain alterations and their association with pain sensitization in patients with FS.
Materials and methods
A total of 54 FS patients and 52 healthy controls (HCs) were included in this study. Here, we applied both structural and functional magnetic resonance imaging (MRI) techniques to investigate brain abnormalities in FS patients. Voxel-wise comparisons were performed to reveal the differences in the gray matter volume (GMV) and amplitude of low-frequency fluctuation (ALFF) between FS patients and HCs. Furthermore, the region of interest (ROI) to whole-brain functional connectivity (FC) was calculated and compared between groups. Finally, Pearson's correlation coefficients were computed to reveal the association between clinical data and brain alterations.
Results
Four main findings were observed: (1) FS patients exhibited decreased thalamus GMV, which correlated with pain intensity and pain threshold; (2) relative to HCs, FS patients exhibited a higher level of ALFF within the anterior cingulate cortex (ACC) and the thalamus; (3) FS patients exhibited a significant increase in Tha-S1 FC compared to HCs; and (4) the effect of thalamus GMV on pain intensity was mediated by pain threshold in FS patients.
Conclusion
The dysfunctional thalamus might induce pain hypersensitivity, which further aggravates the pain in FS patients.
functional magnetic resonance imagingchronic painfrozen shoulderamplitude of low frequency fluctuationgray matter volumefunctional connectivitysection-at-acceptanceHeadache and Neurogenic PainIntroduction
Frozen shoulder (FS), also referred to as adhesive capsulitis, is a condition that manifests as severe spontaneous shoulder pain and night pain, accompanied by gradual restrictions of both active and passive movements of the affected shoulder (1–3). FS has a prevalence of 2–5% in the general population, with the majority of patients being women between the age of 40 and 65 years (4). This condition has a significant impact on an individual's health and economy, with a duration ranging between 1 and 3 years (5, 6). FS has typically been considered a clinical condition with a dominant pain mechanism of peripheral nociception, wherein increased pro-inflammatory cytokines and activation of the neuroimmune system trigger a subsequent persisting pain and fibrosis of the capsular tissue (7, 8). Recent research, however, suggests that the pathophysiology of FS is complex, and central pain mechanisms have a crucial role in patients with FS (8–12). According to Mercè et al. FS patients experience more extended areas of pain, which is associated with higher levels of pain, pain catastrophizing, and pain sensitization (9). Furthermore, Michel et al. also observed greater allodynia, hyperalgesia, catastrophizing, and hypervigilance in FS patients compared to healthy participants, indicating potential central sensitization for pain in FS (11). As the FS continues over time, it is not surprising that the central nervous system will develop potential neuroplastic changes similar to other chronic pain conditions (13–17). Till now, the implications of central pain sensitization remain unclear in patients with FS and the direct evidence for its existence is still lacking. CS has been ‘defined as an amplification of neural signaling within the central CNS that elicits pain hypersensitivity'. Recent studies on chronic pain have demonstrated an association between cortical alterations and pain hypersensitivity using neuroimaging methods (e.g., resting-state fMRI and arterial spin labeling) (18, 19). These non-invasive and objective methods of brain functional measurement have been proven to be invaluable in gaining a deeper understanding of pain sensitization mechanisms in patient populations and for exploring analgesic targets (20). Furthermore, research studies have revealed regional functional abnormalities [amplitude of low-frequency fluctuation and regional homogeneity (21–23)], alterations in the pain modulation pathway [e.g., pain ascending/descending pathway (24)], and network reorganizations [e.g., default mode network and saliency network (25, 26)], which were associated with pain hypersensitivity in various chronic pain conditions. While previous studies have identified brain neuropathological changes in chronic pain patients, the central mechanisms of pain are yet to be studied in depth in FS. It is, therefore, essential to gain a thorough understanding of central pain mechanisms for an accurate diagnosis and effective treatment of FS. By understanding the mechanisms of pain sensitization, clinicians can develop innovative pain management strategies to manage hypersensitivity in these patients.
We, therefore, aim to elucidate the relationship between brain abnormalities and pain sensitization in FS patients based on resting-state fMRI data. It is hypothesized that FS patients have higher levels of pain sensitivity associated with abnormal brain function compared to healthy adults. Our study investigates the brain functional and structural alterations in FS patients, which may offer insights into the mechanisms of central pain sensitization in FS.
Materials and methodsSubjects
The Institutional Review Board of Tianjin Medical University General Hospital approved the current study, and each participant gave written informed consent before each procedure. A total of 54 primary FS patients along with 52 healthy controls were included during 2022–2023. All participants were right-handed.
The inclusion criteria for FS patients were as follows: (1) the reduction of external rotation of the affected shoulder (i.e., 50% reduction compared to the unaffected shoulder of lower than 30°); (2) 25% reduction in the range of motion of at least two planes of movement compared to the unaffected shoulder; (3) the duration of pain and movement restriction was over at least 3 months; and (4) normal shoulder X-rays. The exclusion criteria were as follows: (1) patients with secondary FS and other shoulder conditions (e.g., fractures, dislocations, arthritis, cervical radiculopathy, and previous surgery) were excluded; (2) other chronic pain conditions including fibromyalgia and rheumatic diseases; (3) other co-morbidities (i.e., cardiovascular, neurological, and cognitive diseases); (4) patients who were taking antidepressants and anticonvulsants; and (5) those unable to finish the fMRI scan.
For healthy participants, the same exclusion criteria applied with the exception of the FS diagnosis.
Clinical assessment
All FS patients were assessed prior to fMRI scanning. All FS patients were instructed clearly to rate their trait pain intensity (i.e., average pain intensity of the last month) using a standardized numerical rating scale (NRS) ranging from 0 to 100 (10 = no pain (warm); 20 = threshold pain; and 100 = intolerable pain) and report the duration of pain (month).
Using the two pain questionnaires, the Pain Catastrophizing Scale (PCS) (27), and the Pain Vigilance and Awareness Questionnaire (PVAQ) (28), we assessed the pain-related behaviors of both FS patients and HCs. The PCS consists of 13 items anchored between 0 (never) and 4 (always) on a 5-point scale. There is an overall score ranging from 0 (representing no pain catastrophizing) to 52, and a higher score indicates a rise in pain catastrophizing. A PVAQ questionnaire consists of 16 items, each with a 6-point scale ranging from 0 (never) to 5 (always). With the total score ranging from 0 (minimal attention to pain) to 100, a higher score indicates a greater level of attention.
To determine the pain threshold for both FS patients and healthy controls, electrical stimulation (0.2-ms square wave pulse; Digi-timer DS-7A, Hertfordshire, England) with a bipolar probe was used, similar to our previous study (29). The anode was placed distally, and the inter-electrode distance between the two electrodes was 20 mm. Sensitization and receptor fatigue can be reduced with this stimulation technique. Based on ascending limits (discarding the first series), stimulus intensities related to sensory detection and pain detection thresholds (pricking sensation) were measured. Participants were instructed to respond verbally if they felt any stimulation, with an expected response of a pricking sensation corresponding to Aδ-fiber activation. As only detection thresholds were assessed, no rating scale was used.
Data acquisition
A 3T MR scanner (MAGNETOM Prisma, Siemens, Erlangen, Germany) with a 64-channel phase-array head-neck coil was used for obtaining fMRI data. To reduce unconscious movement, participants were given a sponge pad for head support and instructed to stay still. Additionally, participants were told to close their eyes and keep their thoughts neutral, while remaining awake. The simultaneous multi-slice gradient-echo echo-planar imaging (EPI) sequence was used for fMRI scanning using the following parameters: repetition time (TR) = 800 ms, echo time (TE) = 30 ms, in-plane resolution = 3 mm × 3 mm, field of view (FOV) = 222 mm × 222 mm, flip angle (FA) = 54 degrees, matrix = 74 × 74, slice thickness = 3 mm, number of slices = 48, bandwidth = 1,690 Hz/pixel, no gap, PAT (parallel acquisition technique) mode, slice orientation = transversal, slice acceleration factor = 4, and phase encoding acceleration factor = 2. A total of 450 functional images were taken in 6 min. A high-resolution 3D T1 structural image was acquired using the two-inversion contrast magnetization prepared rapid gradient echo sequence (MP2RAGE), with a duration of 6 min and 42 s. The parameters used were inversion times (TI1/TI2) = 700 ms/2,110 ms, TR/TE = 4,000 ms/3.41 ms, matrix = 256 × 240, FA1/FA2 = 4 degree/5 degree, FOV = 256 mm × 240 mm, number of slices = 192, slice thickness = 1 mm, slice orientation = sagittal, and in-plane resolution = 1 mm × 1 mm.
Preprocessing steps
The Data Processing Assistant for rs-fMRI (DPARSF; http://www.restfmri.net/forum/DPARSF) toolbox was used in our current study for data preprocessing. The preprocessing steps were as follows: (1) Due to the acclimatization to the scanner environment, magnetization stabilization, the first ten timepoints were excluded from each functional scan; (2) in order to eliminate the effect of head movement, motion corrections were performed; (3) brain functional images were first coregistered to T1 images and then normalized to the standard space using the Montreal Neurological Institute (MNI) template; (4) the voxels of functional images were resampled to 3 × 3 × 3 mm3; after normalization, the normalized images were visually checked; (5) the linear drift, along with physiological noise (e.g., mean global signal, the white matter signal, and the CSF signal) and friston-24 parameters, were regressed out to minimize non-neural signals as covariates; (6) subsequently, a scrubbing procedure was conducted on timepoints with high motion; and (7) finally, the filter step was performed across 0.01–0.08 Hz to remove high-frequency noise and then smoothed with a 5-mm full-width-half-maximum isotropic Gaussian kernel. The resulting data were used for further analysis.
In our current study, two FS patients were excluded due to excessive head motion during fMRI scanning; therefore, a total of 52 FS patients and 52 healthy participants were included for further analysis.
Brain functional and structural metricsALFF
To compute the amplitude of low-frequency fluctuations based on resting-state fMRI data, the time series of a given voxel was extracted, and the fast Fourier transforms were performed to transform the time series into frequency domains. ALFF was calculated by averaging the square root of the power spectrum across 0.01–0.08 Hz. After calculating the ALFF values, the results were Z-scores and regressed out the demographic information (e.g., age, gender, and education years) for statistical analysis.
Gray matter volume
Voxel-based morphometry (VBM) analyses were performed to obtain whole-brain gray matter volume in both FS and healthy participants using T1-weighted images. The high-resolution T1-weighted images were processed with the Computational Anatomy Toolbox (CAT12) and SPM12 with the following preprocessing steps: (1) The T1 images were first manually set to the anterior commissure orientation for better registration after screening for artifacts and gross anatomical abnormalities; (2) the reorientated images were segmented into the gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) after signal inhomogeneities correction; (3) DARTEL was used with non-linear registration to standard space, and intensity modulation was conducted using the linear and non-linear components of the Jacobian determinant derived from the deformation fields; and (4) the intracranial volume was calculated by summing the volumes of the segmented gray matter, white matter, and cerebrospinal fluid compartments.
Functional connectivity
After performing the between-group comparison of the ALFF and GMV for identifying significant regions, a follow-up functional connectivity (FC) analysis was conducted on three ROIs. First, the resultant cluster of VBM analysis was first downsampled and registered to functional space (61 × 73 × 61). Subsequently, the overlap between significant clusters of structural and functional analysis was used as a region of interest for functional connectivity analysis. The mean time series across all voxels in the ROIs were extracted, and the voxel-wise functional connectivity was calculated. The resultant ROI-to-whole-brain FC maps were then Z-scored for further statistical analyses. The Data Processing Assistant for rs-fMRI (DPARSF; http://www.restfmri.net/forum/DPARSF) toolbox was used for FC analysis.
Statistical analyses
Voxel-wise two-sample t-tests were performed to compare the ALFF, FC, and GMV differences in a gray matter mask between FS patients and healthy controls (HCs) (P < 0.05, with false discovery rate correction using SPM12, http://www.fil.ion.ucl.ac.uk/spm). The gray matter mask was generated based on the anatomical automatic labeling (AAL) template. Correlation analyses were performed to reveal the association among clinical measures. To further explore the association between brain alterations and clinical measures, Pearson's correlation coefficients were computed for the FS group in brain regions that exhibited significant between-group differences.
Mediation analysis
Furthermore, after performing the between-group comparison of the ALFF and GMV for identifying a significant association between clinical metrics and brain abnormalities in the FS group, the mediatory relationship was assessed using a bootstrapped mediation analysis of the GMV with left thalamus (x, y, z:), pain threshold, and pain intensity similar to a previous study (24). The PROCESS macro (www.processmarcro.org, version 2.16.3) in Statistical Product Service Solutions (SPSS; IBM, version 23.0) was used with 2000 bootstrap samples, which identified 95% confidence intervals for model components. The mediation analysis aimed to evaluate whether there was a significant difference between the total effect (path c) and the direct effect (path c′) that account for the mediator (M). Taking the pain threshold as the mediator, we tested the model with thalamus GMV as the independent variable and pain intensity ratings as the dependent variable based on the previously illustrated pathway, such that the atrophy of the thalamus increased the sensitivity to pain in chronic patients. A significant mediation occurs when bootstrapped upper and lower 95% confidence intervals (CIs) do not include zero.
ResultsDemographic data
Demographic information and clinical evaluations are displayed in Table 1. There were no significant differences between the FS patients and healthy participants in terms of age, gender, or years of education (p > 0.05).
Demographic data of the current study.
FS
HC
P-value
T and χ2 value
Age
45.4 ± 10.3
43.2 ± 6.7
0.19
1.29
Gender (F/M)
26/26
26/26
1
0
Education (years)
8.6 ± 4.1
9.1 ± 6.5
0.64
0.42
Side of pain (left/right/bilateral)
(20/24/8)
0
N/A
N/A
PT
8.1 ± 2.1
12.2 ± 2.0
< 0.001
10.23
PVAQ
44.5 ± 6.7
32.6 ± 7.9
< 0.001
8.27
PCS
24.33 ± 6.3
17.8 ± 6.9
< 0.001
5.03
Trait pain intensity
57.2 ± 10.4
N/A
N/A
N/A
FS, frozen shoulder patients; HC, healthy controls; F, female; M, male; PT, pain threshold; PVAQ, Pain Vigilance and Awareness Questionnaire; PCS, Pain Catastrophic Scale; trait pain intensity: measured with numerous rating scales ranging from 0 to 100.
Clinical assessment
Relative to HCs, FS patients exhibited a significantly higher level of PCS and PVAQ and a significantly lower pain threshold (Table 1, Figure 1A). The association among clinical measures was illustrated in a heat map (Figure 1B). A mild positive correlation was observed between PCS and PVAQ, and a negative correlation was observed between pain threshold and pain intensity in FS patients. The scatter plots are also illustrated in Figure 1C.
(A) Differences in the Pain Catastrophic Scale (PCS), Pain Vigilance and Awareness Questionnaire (PVAQ), and pain threshold (PT) between frozen shoulder (FS) and healthy controls (HC). ****P < 0.001. (B) left. Heat map for association among PCS, PVAQ, PT, and pain intensity (NRS) in FS patients. (B) right. Heat map for association among PCS, PVAQ, and PT in HC patients. (C) Scatter plots for the significant correlation between PT and pain intensity, between PVAQ and PCS, and between PVAQ and pain intensity.
ALFF, GMV, and ROI-to-whole-brain FC
In the current study, we observed significant decreases in GMV within the bilateral thalamus in FS patients relative to HCs (Figure 2A, Table 2). Compared to HCs, FS patients also exhibited a significantly higher level of ALFF within the left thalamus and the bilateral anterior cingulate cortex (Figure 2B, Table 3). Furthermore, the altered GMV within the thalamus was significantly correlated with the pain intensity (R = 0.56, P < 0.001) and pain threshold (R = −0.32, P = 0.02) in FS patients (Figures 3A, B).
(A) Brain regions exhibited decreased gray matter volume (GMV) in the frozen shoulder (FS) patients relative to healthy controls (HC). (B) Brain regions exhibited increased amplitude of low-frequency fluctuation (ALFF) in FS relative to HC. (C) Intersection for structural and functional alterations in thalamus. The intersection was used for functional connectivity (FC) analysis. (D) Brain regions exhibited increased FC in the frozen shoulder (FS) patients relative to healthy controls (HC).
Gray matter volume (GMV) differences between frozen shoulder (FS) patients and healthy controls (HC).
Brain regions
Peak MNI (x, y, z)
Voxels
T-value
Bilateral thalamus
−3
−6
9
325
−11.45
The clusters exhibited significant between-group differences in GMV between FS and HC revealed by voxel-wise two-sample t-test.
The amplitude of low-frequency fluctuation (ALFF) differences between frozen shoulder (FS) patients and healthy controls (HC).
Brain regions
Peak MNI (x, y, z)
Voxels
T-value
Left thalamus
−3
−12
0
66
10.85
Right thalamus
3
−15
12
64
9.63
Bilateral ACC
1
12
30
54
8.82
The clusters exhibited significant between-group differences in ALFF between FS and HC revealed by voxel-wise two-sample t-test. ACC: anterior cingulate cortex.
Association between brain alterations and clinical measurements. (A) Scatter plot for a positive association between thalamus gray matter volume (GMV) and pain threshold (PT). (B) Scatter plot for a negative association between the thalamus GMV and pain intensity. (C) Scatter plot for a negative association between the thalamus GMV and amplitude of low-frequency fluctuation (ALFF). (A) Scatter plot for a positive association between the anterior cingulate cortex (ACC), ALFF, and pain vigilance and awareness questionnaire (PVAQ).
We found that in the right thalamus, the peak coordinates for significant clusters of both GMV and ALFF were close. Therefore, correlation analyses were performed to reveal the association between GMV and ALFF (i.e., the mean values for both GMV and ALFF were extracted within the intersection of two clusters; Figure 2C, Table 4). A relatively mild negative correlation was observed in FS patients (R = −0.30, P = 0.03, Figure 3C). However, no significant correlation was observed between ALFF and pain intensity and between ALFF and pain threshold. We found that the PVAQ was positively correlated with the ALFF within ACC in FS patients (R = 0.35, P = 0.01, Figure 3D).
Thalamus-to-cortical functional connectivity (FC) differences between frozen shoulder (FS) patients and healthy controls (HC).
Brain regions
Peak MNI (x, y, z)
Voxels
T-value
Left S1
−54
−24
54
126
11.75
The clusters exhibited significant between-group differences in FC between FS and HC, revealed by voxel-wise two-sample t-test.
For ROI-to-whole-brain FC, we found that the FC between the left thalamus and the left postcentral gyrus (Tha-S1) was significantly increased in FS patients and correlated with pain threshold in FS patients, while no significant association between pain intensity and Tha-S1 FC was observed.
Mediation analyses
We found that the effect of the thalamus GMV on pain intensity was mediated by pain threshold in FS patients (direct effect = −0.16; indirect effect = −3.21, p < 0.05; 95% confidence interval: [−6.12, −1.06], Figure 4A).
(A) Medication analyses. The effect of GMV on pain intensity was mediated by pain threshold in FS patients. (B) The possible assumption for pain hypersensitivity in FS patients based on our current findings.
Discussion
In this study, four main findings were observed: (1) FS patients exhibited hypersensitivity to pain, which was correlated with the GM atrophy within the thalamus compared to HCs; (2) relative to HCs, FS patients exhibited a higher level of ALFF within the ACC and the thalamus; (3) relative to HCs, FS patients exhibited a significant increase in Tha-S1 FC; and (4) the effect of the thalamus GMV on pain intensity was mediated by pain threshold in FS patients.
Compared with HCs, we found a significantly higher level of PCS and PVAQ ratings in FS patients, along with a lower pain threshold (i.e., higher pain sensitivity.). In patients with chronic pain, the pain negatively impacts health-related quality of life (30, 31). Previous studies have reported that the pain of FS is recurrent and frequently progresses to the chronic stage. Pain catastrophizing is considered a negative mental set during anticipated or actual painful experiences and has been shown to be increased in FS patients (9). As for pain vigilance, the PVAQ has been demonstrated to be a reliable instrument for the evaluation of vigilance and attention to pain (32–34). It assesses various aspects of an individual's awareness, including consciousness, vigilance, and observation of pain symptoms, with higher scores indicating a higher level of such behaviors. In patients with chronic pain, preoccupation with pain is associated with pain-related fear and perceived pain severity. However, in our current study, no significant association was observed between pain intensity and PVAQ or between pain intensity and PCS in FS patients. Similar to previous findings, our observed hypervigilance in FS patients indicated that these patients might immerse themselves in pain (32). Furthermore, previous research studies have shown that patients with chronic pain have a significantly lower pain threshold compared with HCs (35–38). Castaldo et.al. compared the pressure pain thresholds (PPTs) of patients with chronic pain and HCs (39). They found that PPT is reliable and able to discriminate between individuals with and without pain, indicating an increased sensitivity to pain in patients. Similarly, this phenomenon has been extensively reported in various pain conditions, including postherpetic neuralgia (40), temporomandibular disorders (41), and migraine (42). Our current result further extended previous findings, indicating that FS patients experienced persistent shoulder pain and developed sensitization to pain. Interestingly, we also observed a positive correlation between pain intensity and pain threshold. This result is in line with the previous report (43), suggesting that pain sensitization could further worsen the pain in patients with chronic pain.
We observed a significant decrease in GMV in the thalamus and an increase in ALFF in the thalamus and the anterior cingulate cortex (ACC). The Tha-S1 FC was also increased in FS patients. Furthermore, the GMV reduction in the thalamus was correlated with pain intensity and pain threshold in FS patients. The thalamus, which is the primary relay station for afferent transmission of nociceptive information to cortical regions, has been shown to be essential in developing and maintaining neuropathic pain (44–46) (e.g., chronic back pain and trigeminal neuralgia). Thalamus atrophy has been shown in various chronic pain conditions in prior investigations. Previous research studies have also shown that the thalamus anatomy was associated with neuropathic pain, and the level of atrophy was correlated with pain intensity in chronic pain patients (47–49). An explanation for the atrophy of the gray matter in the thalamus could be the overactivation caused by excitotoxins and inflammatory agents (50–52). The reduction in thalamus volume reported here further supports previous findings and may indicate a dysfunction in the ascending pain modulation pathway. In recent years, neuroimaging studies have revealed that the thalamus–cortical pathway has a crucial role in pain processing. In healthy participants, dynamic causal model (DCM) analysis has shown distinct corticothalamic feedback regulations from S1 and S2, showing that S1 generally exerts inhibitory feedback regulation independent of external stimulation, whereas S2 provides additional inhibition to the thalamic activity during nociceptive information processing in humans (18). FC analysis based on rs-fMRI data has revealed an increased thalamus to S1 (Tha-S1) and thalamus to S2 FC in chronic pain patients than in HCs (24, 53). The results of our study also suggested that there may be functional deficits in the ascending pain modulation pathway among patients with FS. Accordingly, previous studies have demonstrated thalamocortical FC dysfunction as a neurobiomarker for neuropathic pain (53). Furthermore, it has been considered that thalamic-somatosensory connections may be enhanced by the overload of spontaneous pain in thalamocortical networks, which might reflect that FS patients immersed themselves in a chronic pain state (53). Taken together, our results indicate that the long-term nociceptive input might cause the overactivation of the thalamus, leading to thalamus atrophy and enhanced Tha-S1 connection in FS patients. Moreover, such brain alterations might be crucial for pain sensitization in FS patients (Figure 4B). To further test this hypothesis, mediation analysis was performed to reveal the association between pain intensity, pain threshold, and thalamus GMV. Interestingly, mediation analyses revealed that the effect of pain sensitivity on pain intensity was mediated by the thalamus GMV. According to the definition of central sensitization (i.e., amplification of neural signaling within the central CNS that elicits pain hypersensitivity), our mediation analyses further give a possible causal explanation for brain abnormalities that elicits pain hypersensitivity in FS patients. Therefore, our current finding provides further evidence for pain sensitization in FS patients.
It is noteworthy that our study has several limitations. First, all the participants received analgesics before enrolling in our current study. There is a possibility that this may have affected our results. Second, there are heterogenous sub-regions within the thalamus, and in our current study, we have not explored the functional and structural alterations in sub-regions of the thalamus. This limited the illustration of detailed mechanisms. Third, the question remains as to whether the functional and structural alterations are a “brain signature” of FS or whether they are shared by other chronic pain conditions. It remains unclear whether pain is sensitized at the spinal cord level, which can be clarified with spinal cord functional magnetic resonance imaging. Finally, vertex analysis (FSL-FIRST vertex) would be of assistance in illustrating which part of the thalamus is the most affected. In future experiments, we will pay particular attention to this.
Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Ethics statement
The studies involving human participants were reviewed and approved by Ethics Committee of Tianjin Hospital. The patients/participants provided their written informed consent to participate in this study.
Author contributions
XC and JZ designed the study. TL, RZ, and JL analyzed the data and wrote the manuscript. All authors contributed to the article and approved the submitted version.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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