AUTHOR=Zhang Shaohui , Yuan Liu , Liu Chunxiu , Kuang Suhui , Wang Jiaqi , Liang Shuli , Cong Ming 

TITLE=Conduction treatment of temporal lobe epilepsy in rats: the dose-effect relationship between current resistance and therapeutic effect

JOURNAL=Frontiers in Neurology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2023.1181953

DOI=10.3389/fneur.2023.1181953

ISSN=1664-2295

ABSTRACT=Objective: To investigate the effect of current resistance on therapeutic outcomes, and the mechanism of current conduction treatment in a rat model of temporal lobe epilepsy (TLE). Methods: Rats were randomly divided into three groups: control; low-resistance conduction (LRC); and high-resistance conduction (HRC). The content of Glu/GABA in the hippocampus was determined using a neurotransmitter analyzer. mRNA and protein expression of interleukin 1β (IL-1β) /IL-1 receptor 1(IL-1R1) and high mobility group protein B1 (HMGB-1) / toll-like receptor-4 (TLR-4) in hippocampal neurons were tested. Video electroencephalogram monitoring was used to record seizures and EEG discharges. Cognitive function in the rats was tested using the Morris water maze. Results: Glutamate/GABA ratio in the control and HRC group was significant differences from LRC groups. The levels of HMGB1/TLR4 (P<0.01) and IL-1β/IL-1R1 (P<0.05) in the LRC group were significantly lower than those in the HRC group. The mRNA levels of TLR4 (P<0.05) and IL-1β (P<0.01) in the HRC group were significantly lower than those in the control group. The frequency of total and propagated seizures was lower in the LRC group than in the control and HRC groups (P<0.01). The number of platform crossings in the LRC group was significantly higher than those in the control and HRC groups in the space exploration experiment. Conclusion: Current resistance affected seizure control and cognitive protection in rats with TLE treated with current conduction. Glutamate /GABA, IL-1β/IL-1R1, and TLR-4 may participate in the anti-seizure mechanism of current conduction treatment.