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<journal-id journal-id-type="publisher-id">Front. Neurol.</journal-id>
<journal-title>Frontiers in Neurology</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Neurol.</abbrev-journal-title>
<issn pub-type="epub">1664-2295</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
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<article-meta>
<article-id pub-id-type="doi">10.3389/fneur.2024.1421655</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Neurology</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Association of higher triglyceride&#x2013;glucose index and triglyceride-to-high-density lipoprotein cholesterol ratio with early neurological deterioration after thrombolysis in acute ischemic stroke patients</article-title>
</title-group>
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<name><surname>Deng</surname> <given-names>Mingzhu</given-names></name>
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<name><surname>Song</surname> <given-names>Kangping</given-names></name>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Xu</surname> <given-names>Wei</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="author-notes" rid="fn0001"><sup>&#x2020;</sup></xref>
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<name><surname>He</surname> <given-names>Guohua</given-names></name>
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<contrib contrib-type="author">
<name><surname>Hu</surname> <given-names>Jue</given-names></name>
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<contrib contrib-type="author">
<name><surname>Xiao</surname> <given-names>Hui</given-names></name>
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<name><surname>Zhou</surname> <given-names>Nina</given-names></name>
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<name><surname>Chen</surname> <given-names>Sufen</given-names></name>
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<name><surname>Xu</surname> <given-names>Guilan</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<contrib contrib-type="author">
<name><surname>Tong</surname> <given-names>Yangping</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<name><surname>Zhang</surname> <given-names>Dan</given-names></name>
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<name><surname>Wang</surname> <given-names>Zhen</given-names></name>
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<name><surname>Li</surname> <given-names>Fangyi</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
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<aff id="aff1"><sup>1</sup><institution>Department of Neurology, Brain Hospital of Hunan Province, The Second People&#x2019;s Hospital of Hunan Province</institution>, <addr-line>Changsha</addr-line>, <country>China</country></aff>
<aff id="aff2"><sup>2</sup><institution>Department of Neurology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China</institution>, <addr-line>Changsha</addr-line>, <country>China</country></aff>
<author-notes>
<fn fn-type="edited-by" id="fn0002">
<p>Edited by: Raffaele Ornello, University of L&#x2019;Aquila, Italy</p>
</fn>
<fn fn-type="edited-by" id="fn0003">
<p>Reviewed by: Zilong Hao, Sichuan University, China</p>
<p>Lingling Zhang, Yangzhou University, China</p>
</fn>
<corresp id="c001">&#x002A;Correspondence: Zhen Wang, <email>docwz2007@163.com</email></corresp>
<corresp id="c002">Fangyi Li, <email>475040942@qq.com</email></corresp>
<fn fn-type="equal" id="fn0001">
<p><sup>&#x2020;</sup>These authors have contributed equally to this work and share first authorship</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>21</day>
<month>08</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="collection">
<year>2024</year>
</pub-date>
<volume>15</volume>
<elocation-id>1421655</elocation-id>
<history>
<date date-type="received">
<day>22</day>
<month>04</month>
<year>2024</year>
</date>
<date date-type="accepted">
<day>06</day>
<month>08</month>
<year>2024</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x00A9; 2024 Deng, Song, Xu, He, Hu, Xiao, Zhou, Chen, Xu, Tong, Zhang, Wang and Li.</copyright-statement>
<copyright-year>2024</copyright-year>
<copyright-holder>Deng, Song, Xu, He, Hu, Xiao, Zhou, Chen, Xu, Tong, Zhang, Wang and Li</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<sec id="sec1">
<title>Background</title>
<p>Insulin resistance (IR) can predict the prognosis of patients suffering from cerebrovascular disorders. The triglyceride&#x2013;glucose (TyG) index and triglyceride-to-high-density lipoprotein cholesterol (TG/HDL-C) ratio have been confirmed to be easy and reliable indicators of IR. However, the relationships between the TyG index or TG/HDL-C ratio and early neurological deterioration (END) after thrombolysis in patients with acute ischemic stroke (AIS) are uncertain.</p>
</sec>
<sec id="sec2">
<title>Methods</title>
<p>A retrospective analysis of 1,187 patients diagnosed with AIS who underwent intravenous thrombolysis between January 2018 and February 2024 was performed. Post-thrombolysis END was defined as an increase in the National Institutes of Health Stroke Scale (NIHSS) score of &#x2265;4 within 24&#x2009;h after thrombolysis. Logistic regression analysis was performed to explore the relationships of the TyG index and TG/HDL-C ratio with post-thrombolysis END. Receiver operating characteristic (ROC) analysis was used to assess the ability of the TyG index and TG/HDL-C ratio to discriminate post-thrombolysis END.</p>
</sec>
<sec id="sec3">
<title>Results</title>
<p>Among the 1,187 recruited patients, 179 (15.08%) were diagnosed with post-thrombolysis END, and 1,008 (84.92%) were diagnosed with non-END. A binary logistic regression model indicated that the TyG index (odds ratio [OR], 2.015; 95% confidence interval [CI] 1.964&#x2013;2.414, <italic>p</italic>&#x2009;=&#x2009;0.015) and TG/HDL-C ratio (OR, 1.542; 95% CI, 1.160&#x2013;2.049, <italic>p</italic>&#x2009;=&#x2009;0.004) were independent factors for post-thrombolysis END. The area under the curve (AUC) values for the TyG index, TG/HDL-C ratio, and TyG index combined with the TG/HDL-C ratio for post-thrombolysis END were 0.704, 0.674, and 0.755, respectively.</p>
</sec>
<sec id="sec4">
<title>Conclusion</title>
<p>This study indicates that the TyG index and TG/HDL-C ratio can be used as prognostic factors to predict post-thrombolysis END.</p>
</sec>
</abstract>
<kwd-group>
<kwd>acute ischemic stroke</kwd>
<kwd>intravenous thrombolysis</kwd>
<kwd>triglyceride&#x2013;glucose index</kwd>
<kwd>triglyceride-to-high-density lipoprotein cholesterol ratio</kwd>
<kwd>early neurological deterioration</kwd>
</kwd-group>
<counts>
<fig-count count="4"/>
<table-count count="3"/>
<equation-count count="0"/>
<ref-count count="43"/>
<page-count count="9"/>
<word-count count="5808"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Neurological Biomarkers</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="sec5">
<title>Introduction</title>
<p>Acute ischemic stroke (AIS), which is caused by sudden arterial blockage and results in neuronal damage, is the most common type of stroke (<xref ref-type="bibr" rid="ref1">1</xref>, <xref ref-type="bibr" rid="ref2">2</xref>). The preferred treatment for AIS is intravenous recombinant tissue plasminogen activator in the early phase (&#x2264;4.5&#x2009;h) (<xref ref-type="bibr" rid="ref3">3</xref>, <xref ref-type="bibr" rid="ref4">4</xref>). Nevertheless, a minority of patients continue to experience early neurological deterioration (END) in which neurological impairments and symptoms intensify within 24&#x2009;h after thrombolysis (<xref ref-type="bibr" rid="ref5">5</xref>). END is associated with an increased risk of mortality and morbidity, and previous studies have shown that END is relevant to unfavorable long-term outcomes in AIS patients (<xref ref-type="bibr" rid="ref6">6</xref>, <xref ref-type="bibr" rid="ref7">7</xref>). Therefore, it is important to investigate the risk factors and measurable indicators of post-thrombolysis END in AIS patients.</p>
<p>Insulin resistance (IR) is considered the primary pathophysiology of metabolic syndrome (<xref ref-type="bibr" rid="ref8">8</xref>), which is involved in the pathogenesis of cerebrovascular diseases, mainly through endogenous fibrinolytic dysfunction, thrombosis, elevated platelet activation, and inflammation (<xref ref-type="bibr" rid="ref9">9</xref>). The hyperinsulinemic&#x2013;euglycemic clamp test is the gold standard for assessing IR. However, the high cost of this measurement limits its wide-scale clinical applicability (<xref ref-type="bibr" rid="ref10">10</xref>). In recent years, the triglyceride&#x2013;glucose (TyG) index and triglyceride-to-high-density lipoprotein cholesterol (TG/HDL-C) ratio have been established as reliable, cost-effective, and easily accessible surrogate markers for IR (<xref ref-type="bibr" rid="ref11">11</xref>&#x2013;<xref ref-type="bibr" rid="ref15">15</xref>). According to large cohort studies, the TyG index might be a useful IR biomarker for predicting the prognosis of stroke patients (<xref ref-type="bibr" rid="ref16">16</xref>). A higher TyG index is associated with more severe END in AIS patients (<xref ref-type="bibr" rid="ref11">11</xref>). Moreover, prior research has demonstrated that an elevated TyG index is associated with poor outcomes after thrombolysis (<xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref17">17</xref>, <xref ref-type="bibr" rid="ref18">18</xref>). However, there are conflicting relationships between metabolic syndrome and outcomes after thrombolysis (<xref ref-type="bibr" rid="ref19">19</xref>, <xref ref-type="bibr" rid="ref20">20</xref>). Therefore, the relationship between the TyG index and post-thrombolysis END deserves further investigation. The TG/HDL-C ratio is an easily accessible serum biomarker and may be utilized for assessing IR (<xref ref-type="bibr" rid="ref13">13</xref>). Previous studies have shown significant associations between the TG/HDL-C ratio and incident cardiovascular disease (<xref ref-type="bibr" rid="ref13">13</xref>, <xref ref-type="bibr" rid="ref21">21</xref>). Nevertheless, a recent large-scale cohort study revealed that there was no significant correlation between TG/HDL-C and worse cardiovascular disease outcomes (<xref ref-type="bibr" rid="ref12">12</xref>). Furthermore, few studies have investigated the correlation between the TG/HDL-C ratio and post-thrombolysis END.</p>
<p>The early neurological outcome after thrombolysis is related to the long-term prognosis of patients (<xref ref-type="bibr" rid="ref7">7</xref>, <xref ref-type="bibr" rid="ref22">22</xref>). The correlation between the TyG index or HDL-C ratio and post-thrombolysis END remains unclear. Therefore, we investigated the associations of the TyG index and TG/HDL-C ratio with the risk of post-thrombolysis END.</p>
</sec>
<sec sec-type="materials|methods" id="sec6">
<title>Materials and methods</title>
<sec id="sec7">
<title>Study design and participants</title>
<p>Patients with AIS who received intravenous thrombolysis within 4.5&#x2009;h were selected from Changsha Central Hospital and Hunan Province Second People&#x2019;s Hospital. AIS was diagnosed using head imaging techniques, including CT and MRI, based on the 2018 Chinese guidelines for the diagnosis and treatment of acute ischemic stroke. The diagnostic criteria were as follows: (1) acute onset; (2) focal neurological deficits (weakness or numbness of one side of the face or limb, language disorder, etc.), a few of which are global neurological deficits; (3) imaging liability lesions or symptoms/signs for more than 24&#x2009;h; (4) exclusion of non-vascular causes; and (5) brain CT/MRI exclusion of cerebral hemorrhage (<xref ref-type="bibr" rid="ref23">23</xref>). The inclusion criteria were as follows: (1) admission within 4.5&#x2009;h after onset, (2) treatment with intravenous thrombolysis with r-tPA, and (3) age 18&#x2009;years or older. The exclusion criteria for patients were as follows: (1) discharged within 24&#x2009;h, (2) intravenous thrombolysis was interrupted due to serious side effects, (3) incomplete clinical data, and (4) cerebral vascular interventional therapy. This study was approved by the Ethics Committee of Changsha Central Hospital and Hunan Province Second People&#x2019;s Hospital. Between January 2018 and February 2024, 1,187 AIS patients were recruited. <xref ref-type="fig" rid="fig1">Figure 1</xref> shows a detailed flow diagram for patient enrollment.</p>
<fig position="float" id="fig1">
<label>Figure 1</label>
<caption>
<p>Study flow diagram. AIS, acute ischemic stroke; END, early neurological deterioration.</p>
</caption>
<graphic xlink:href="fneur-15-1421655-g001.tif"/>
</fig>
</sec>
<sec id="sec8">
<title>Data collection</title>
<p>Expert neurologists conducted the clinical evaluations in a blinded manner. The following information was collected from each participant: age, sex, body mass index, risk factors for stroke (hypertension, diabetes mellitus, atrial fibrillation, coronary artery disease, and current drinking and smoking), and clinical features (admission stroke onset severity, admission systolic blood pressure (SBP), diastolic blood pressure (DBP), onset to treatment time (OTT), and stroke subtypes). Computed tomography, magnetic resonance, electrocardiography, echocardiography, carotid ultrasonography, and transcranial Doppler were used to determine the stroke subtype. The stroke subtype was classified according to the Trial of Org 10172 in Acute Stroke Treatment criteria (<xref ref-type="bibr" rid="ref24">24</xref>). Using a standardized case report form, the demographic information, baseline clinical parameters, clinical diagnoses, and treatment plans were meticulously gathered. The physicians or other healthcare professionals who had been in charge were consulted if any information was unclear.</p>
<p>Blood samples from all patients were collected at 6&#x2013;7&#x2009;a.m. on the day after the patients had fasted for at least 8&#x2009;h. In total, 2&#x2009;mL of EDTA-anticoagulated whole blood was used for routine blood tests (automated hematology analyzer, BZ6800, China). In total, 5&#x2009;mL of coagulant-containing blood was used for standard biochemical examination (automatic analyzer, HITACHI 7600, Japan). Blood samples were evaluated for triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and fasting blood glucose (FBG) levels. The TG/HDL-C ratio was calculated. Each blood test was conducted three times. The following formula was used to define the TyG index: Ln [TG (mg/dL)&#x2009;&#x00D7;&#x2009;FBG (mg/dL) &#x00F7; 2] (<xref ref-type="bibr" rid="ref25">25</xref>).</p>
</sec>
<sec id="sec9">
<title>Definition of post-thrombolysis early neurological deterioration and symptomatic intracranial hemorrhage</title>
<p>The two centers&#x2019; certified neurologists were blinded to our investigation and received unified training for evaluating NIHSS scores. Post-thrombolysis END was defined as an increase in the National Institutes of Health Stroke Scale (NIHSS) score by &#x2265;4 points in the total score within 24&#x2009;h after thrombolysis (<xref ref-type="bibr" rid="ref26">26</xref>, <xref ref-type="bibr" rid="ref27">27</xref>). Symptomatic intracranial hemorrhage (sICH) was defined as clinical worsening of at least 4 points on the National Institutes of Health Stroke Scale (NIHSS) score within 24&#x2009;h after thrombolysis, attributed to parenchymal hematoma, subarachnoid, or intraventricular hemorrhage (<xref ref-type="bibr" rid="ref28">28</xref>).</p>
</sec>
<sec id="sec10">
<title>Statistical analysis</title>
<p>SPSS 25.0 (IBM SPSS Statistics software, Version 25.0) was used for data analysis. The Kolmogorov&#x2013;Smirnov test was used to determine if all the data had a normal distribution. If the continuous variables were regularly distributed, they are shown as the means &#x00B1; SDs; if not, they are shown as medians (quartiles). For categorical factors, the results are shown as percentages. The chi-square test or Fisher&#x2019;s exact test was used for categorical variables, and Student&#x2019;s <italic>t</italic>-test or the Mann&#x2013;Whitney U test was used to evaluate differences in the baseline characteristics of continuous variables across groups. Logistic regression analysis was used to detect risk factors for post-thrombolysis END. A MedCalc 15.6.0 (MedCalc Software Acacialaan 22, B-8400 Ostend, Belgium) packet program was used to obtain a receiver operating characteristic (ROC) curve to test the overall ability of the TyG index and TG/HDL-C ratio to discriminate post-thrombolysis END. A two-tailed value of <italic>p</italic>&#x2009;&#x003C;&#x2009;0.05 was considered significant.</p>
</sec>
</sec>
<sec sec-type="results" id="sec11">
<title>Results</title>
<sec id="sec12">
<title>Clinical and demographic characteristics of AIS patients with post-thrombolysis END and non-END</title>
<p><xref ref-type="table" rid="tab1">Table 1</xref> shows the clinical and demographic characteristics of the patients in detail. The baseline characteristics of the AIS patients from the two hospitals are shown in <xref rid="SM1" ref-type="supplementary-material">Supplementary Table S1</xref>. In our study, post-thrombolysis END was observed in 179 patients (15.08%), and post-thrombolysis non-END was observed in 1008 patients (84.92%). In the post-thrombolysis END group, the NIHSS score after rt-PA for 24&#x2009;h (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), DBP (<italic>p</italic>&#x2009;=&#x2009;0.026), diabetes mellitus (<italic>p</italic>&#x2009;=&#x2009;0.014), FBG (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), TG (<italic>P</italic>&#x2009;=&#x2009;&#x003C;0.001), TC (<italic>p</italic>&#x2009;=&#x2009;0.003), TyG index (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and TG/HDL-C ratio (<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001) were significantly greater than those in the post-thrombolysis non-END group, whereas HDL-C (<italic>p</italic>&#x2009;=&#x2009;0.012) was significantly lower than those in the post-thrombolysis non-END group. In the post-thrombolysis END group, the percentage of patients with symptomatic intracranial hemorrhage (sICH) was 26.82% (48/179). In addition, stroke subtype (<italic>p</italic>&#x2009;=&#x2009;0.011) was significantly different between the two groups. <xref ref-type="fig" rid="fig2">Figure 2</xref> shows the TyG index, TG/HDL-C ratio, FBG, TG, TC, and HDL-C for the two groups.</p>
<table-wrap position="float" id="tab1">
<label>Table 1</label>
<caption>
<p>Characteristics of AIS patients with post-thrombolysis END and non-END patients.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variable</th>
<th align="center" valign="top">END (<italic>n</italic> =&#x2009;179)</th>
<th align="center" valign="top">Non-END (<italic>n</italic> =&#x2009;1,008)</th>
<th align="center" valign="top">T/Z</th>
<th align="center" valign="top"><italic>P</italic></th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" colspan="5"><bold>Demographic characteristics</bold></td>
</tr>
<tr>
<td align="left" valign="top">Age, years</td>
<td align="center" valign="top">67.32&#x2009;&#x00B1;&#x2009;12.18</td>
<td align="center" valign="top">66.39&#x2009;&#x00B1;&#x2009;12.40</td>
<td align="center" valign="top">0.094</td>
<td align="center" valign="top">0.348</td>
</tr>
<tr>
<td align="left" valign="top">Male, <italic>n</italic> (%)</td>
<td align="center" valign="top">118 (65.92)</td>
<td align="center" valign="top">622 (61.71)</td>
<td align="center" valign="top">1.515</td>
<td align="center" valign="top">0.283</td>
</tr>
<tr>
<td align="left" valign="top">BMI, kg/m<sup>2</sup></td>
<td align="center" valign="top">23.16&#x2009;&#x00B1;&#x2009;4.75</td>
<td align="center" valign="top">22.99&#x2009;&#x00B1;&#x2009;4.10</td>
<td align="center" valign="top">&#x2212;0.434</td>
<td align="center" valign="top">0.665</td>
</tr>
<tr>
<td align="left" valign="top" colspan="5"><bold>Clinical assessment</bold></td>
</tr>
<tr>
<td align="left" valign="top">NIHSS, score at admission</td>
<td align="center" valign="top">6.5(3&#x2013;12.25)</td>
<td align="center" valign="top">6 (3&#x2013;12)</td>
<td align="center" valign="top">&#x2212;0.711</td>
<td align="center" valign="top">0.477</td>
</tr>
<tr>
<td align="left" valign="top">NIHSS, score after rt-PA 24&#x2009;h</td>
<td align="center" valign="top">8 (5&#x2013;15)</td>
<td align="center" valign="top">3 (1&#x2013;8)</td>
<td align="center" valign="top">&#x2212;8.580</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">sICH, <italic>n</italic> (%)</td>
<td align="center" valign="top">48 (26.82)</td>
<td/>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">SBP, mmHg</td>
<td align="center" valign="top">151.36&#x2009;&#x00B1;&#x2009;24.23</td>
<td align="center" valign="top">147.66&#x2009;&#x00B1;&#x2009;20.30</td>
<td align="center" valign="top">&#x2212;1.386</td>
<td align="center" valign="top">0.167</td>
</tr>
<tr>
<td align="left" valign="top">DBP, mmHg</td>
<td align="center" valign="top">86.85&#x2009;&#x00B1;&#x2009;13.44</td>
<td align="center" valign="top">83.17&#x2009;&#x00B1;&#x2009;12.88</td>
<td align="center" valign="top">&#x2212;2.227</td>
<td align="center" valign="top">0.026</td>
</tr>
<tr>
<td align="left" valign="top">OTT, minute</td>
<td align="center" valign="top">154 (110.5, 215)</td>
<td align="center" valign="top">146 (86, 213)</td>
<td align="center" valign="top">&#x2212;1.129</td>
<td align="center" valign="top">0.259</td>
</tr>
<tr>
<td align="left" valign="top" colspan="5"><bold>Vascular risk factors, <italic>n</italic> (%)</bold></td>
</tr>
<tr>
<td align="left" valign="top">Hypertension</td>
<td align="center" valign="top">111 (62.01)</td>
<td align="center" valign="top">674 (66.87)</td>
<td align="center" valign="top">1.599</td>
<td align="center" valign="top">0.206</td>
</tr>
<tr>
<td align="left" valign="top">Diabetes mellitus</td>
<td align="center" valign="top">49 (27.37)</td>
<td align="center" valign="top">195 (19.35)</td>
<td align="center" valign="top">6.001</td>
<td align="center" valign="top">0.014</td>
</tr>
<tr>
<td align="left" valign="top">Atrial fibrillation</td>
<td align="center" valign="top">26 (14.53)</td>
<td align="center" valign="top">163 (16.17)</td>
<td align="center" valign="top">0.307</td>
<td align="center" valign="top">0.579</td>
</tr>
<tr>
<td align="left" valign="top">Coronary artery disease</td>
<td align="center" valign="top">38 (21.23)</td>
<td align="center" valign="top">218 (21.63)</td>
<td align="center" valign="top">0.014</td>
<td align="center" valign="top">0.905</td>
</tr>
<tr>
<td align="left" valign="top">Current smoking</td>
<td align="center" valign="top">77 (43.02)</td>
<td align="center" valign="top">406 (40.28)</td>
<td align="center" valign="top">0.473</td>
<td align="center" valign="top">0.492</td>
</tr>
<tr>
<td align="left" valign="top">Current drinking</td>
<td align="center" valign="top">36 (20.11)</td>
<td align="center" valign="top">223 (22.12)</td>
<td align="center" valign="top">0.360</td>
<td align="center" valign="top">0.548</td>
</tr>
<tr>
<td align="left" valign="top" colspan="5"><bold>Medication use history, <italic>n</italic> (%)</bold></td>
</tr>
<tr>
<td align="left" valign="top">Previous antiplatelet</td>
<td align="center" valign="top">20 (11.17)</td>
<td align="center" valign="top">145 (14.38)</td>
<td align="center" valign="top">1.310</td>
<td align="center" valign="top">0.252</td>
</tr>
<tr>
<td align="left" valign="top">Previous anticoagulation</td>
<td align="center" valign="top">13 (7.26)</td>
<td align="center" valign="top">71 (7.04)</td>
<td align="center" valign="top">0.011</td>
<td align="center" valign="top">0.916</td>
</tr>
<tr>
<td align="left" valign="top">Previous statin</td>
<td align="center" valign="top">11 (6.15)</td>
<td align="center" valign="top">76 (7.54)</td>
<td align="center" valign="top">0.435</td>
<td align="center" valign="top">0.509</td>
</tr>
<tr>
<td align="left" valign="top">Previous antihypertension</td>
<td align="center" valign="top">69 (38.55)</td>
<td align="center" valign="top">405 (40.18)</td>
<td align="center" valign="top">0.800</td>
<td align="center" valign="top">0.371</td>
</tr>
<tr>
<td align="left" valign="top">Previous hypoglycemic agents</td>
<td align="center" valign="top">24 (13.41)</td>
<td align="center" valign="top">117 (11.61)</td>
<td align="center" valign="top">0.471</td>
<td align="center" valign="top">0.493</td>
</tr>
<tr>
<td align="left" valign="top"><bold>Stroke subtype, <italic>n</italic> (%)</bold></td>
<td/>
<td/>
<td align="center" valign="top">13.014</td>
<td align="center" valign="top">0.011</td>
</tr>
<tr>
<td align="left" valign="top">LAA</td>
<td align="center" valign="top">76 (42.46)</td>
<td align="center" valign="top">320 (31.75)</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">SAO</td>
<td align="center" valign="top">68 (37.99)</td>
<td align="center" valign="top">476 (47.22)</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">CE</td>
<td align="center" valign="top">22 (12.29)</td>
<td align="center" valign="top">168 (16.67)</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">SOE</td>
<td align="center" valign="top">4 (2.23)</td>
<td align="center" valign="top">10 (0.99)</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">SUE</td>
<td align="center" valign="top">9 (5.03)</td>
<td align="center" valign="top">34 (3.37)</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top" colspan="5"><bold>Laboratory data</bold></td>
</tr>
<tr>
<td align="left" valign="top">FBG (mmol/L)</td>
<td align="center" valign="top">6.53 (5.28&#x2013;10.23)</td>
<td align="center" valign="top">5.71 (4.81&#x2013;7.23)</td>
<td align="center" valign="top">&#x2212;5.220</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">TG (mmol/L)</td>
<td align="center" valign="top">1.88(1.15&#x2013;3.9)</td>
<td align="center" valign="top">1.29 (0.94&#x2013;1.82)</td>
<td align="center" valign="top">&#x2212;7.617</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">TC (mmol/L)</td>
<td align="center" valign="top">4.61 (3.78&#x2013;5.39)</td>
<td align="center" valign="top">4.33 (3.7&#x2013;5.01)</td>
<td align="center" valign="top">&#x2212;2.996</td>
<td align="center" valign="top">0.003</td>
</tr>
<tr>
<td align="left" valign="top">HDL-C (mmol/L)</td>
<td align="center" valign="top">1.06&#x2009;&#x00B1;&#x2009;0.32</td>
<td align="center" valign="top">1.12&#x2009;&#x00B1;&#x2009;0.32</td>
<td align="center" valign="top">2.510</td>
<td align="center" valign="top">0.012</td>
</tr>
<tr>
<td align="left" valign="top">LDL-C (mmol/L)</td>
<td align="center" valign="top">2.76&#x2009;&#x00B1;&#x2009;1.02</td>
<td align="center" valign="top">2.74&#x2009;&#x00B1;&#x2009;0.88</td>
<td align="center" valign="top">&#x2212;0.324</td>
<td align="center" valign="top">0.746</td>
</tr>
<tr>
<td align="left" valign="top">TyG index</td>
<td align="center" valign="top">7.82 (7.02&#x2013;8.56)</td>
<td align="center" valign="top">7.13 (6.75&#x2013;7.54)</td>
<td align="center" valign="top">&#x2212;8.728</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">TG/HDL-C</td>
<td align="center" valign="top">2.01 (0.99&#x2013;4.56)</td>
<td align="center" valign="top">1.20 (0.80&#x2013;1.86)</td>
<td align="center" valign="top">&#x2212;7.442</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>BMI, body mass index; sICH, symptomatic intracranial hemorrhage; SBP, systolic blood pressure; DBP, diastolic blood pressure; NIHSS, National Institutes of Health Stroke Scale; OTT, onset to treatment time; LAA, large-artery atherosclerosis; SAO, small-artery occlusion; CE, cardioembolism; SOE, stroke of other determined etiology; SUE, stroke of undetermined etiology; TC, total cholesterol; TG, triglycerides; FBG, fasting blood glucose; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; TyG, triglyceride&#x2013;glucose.</p>
</table-wrap-foot>
</table-wrap>
<fig position="float" id="fig2">
<label>Figure 2</label>
<caption>
<p>Comparisons of the TyG index <bold>(A)</bold>, TG/HDL-C <bold>(B)</bold>, FBG <bold>(C)</bold>, TG <bold>(D)</bold>, TC <bold>(E)</bold>, and HDL-C <bold>(F)</bold> between the END and non-END groups. &#x002A;&#x002A;&#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.001, &#x002A;&#x002A;<italic>p</italic>&#x2009;&#x003C;&#x2009;0.01, &#x002A;<italic>p&#x2009;&#x003C;</italic> 0.05.</p>
</caption>
<graphic xlink:href="fneur-15-1421655-g002.tif"/>
</fig>
</sec>
<sec id="sec13">
<title>Logistic regression analysis for risk factors for post-thrombolysis END</title>
<p>The results of the crude models for post-thrombolysis END are displayed in <xref ref-type="table" rid="tab2">Table 2</xref>. To identify independent risk factors for post-thrombolysis END, the binary logistic regression model included variables with statistical significance mentioned in <xref ref-type="table" rid="tab1">Table 1</xref>. sICH, age, and the National Institutes of Health Stroke Scale (NIHSS) score are also important factors for post-thrombolysis END and should be included in multivariate analysis. There was no collinearity between the TyG index and the TG/HDL-C ratio. However, FBG, TG, and HDL-C were not included in the model because of collinearity with the TyG index and TG/HDL-C ratio. The TyG index (OR, 2.015; 95% CI 1.964&#x2013;2.414, <italic>p</italic>&#x2009;=&#x2009;0.015), TG/HDL-C ratio (OR, 1.542; 95% CI 1.160&#x2013;2.049, <italic>p</italic>&#x2009;=&#x2009;0.004), and sICH (OR, 1.815; 95% CI 1.515&#x2013;2.231, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001) were identified as independent factors for post-thrombolysis END after adjustment for age, initial NIHSS score, DBP, stroke subtype, and TC (<xref ref-type="fig" rid="fig3">Figure 3</xref>). In addition, the TyG index (median&#x2009;=&#x2009;7.17) and TG/HDL-C ratio (median&#x2009;=&#x2009;1.26) were used as binary categorical variables. After adjusting for all confounding factors, the T2 subgroup was still significantly associated with post-thrombolysis END, compared to the T1 subgroup (<xref ref-type="table" rid="tab3">Table 3</xref>).</p>
<table-wrap position="float" id="tab2">
<label>Table 2</label>
<caption>
<p>Logistic regression analysis for risk factors for post-thrombolysis END.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variable</th>
<th align="center" valign="top">OR (95% CI)</th>
<th align="center" valign="top"><italic>P</italic></th>
<th align="center" valign="top">Adjusted OR (95% CI)</th>
<th align="center" valign="top"><italic>P</italic></th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top">Age</td>
<td align="center" valign="top">1.294 (1.081&#x2013;1.307)</td>
<td align="center" valign="top">0.347</td>
<td align="center" valign="top">1.122 (1.041&#x2013;1.255)</td>
<td align="center" valign="top">0.435</td>
</tr>
<tr>
<td align="left" valign="top">Initial NIHSS score</td>
<td align="center" valign="top">1.129 (1.098&#x2013;1.302)</td>
<td align="center" valign="top">0.658</td>
<td align="center" valign="top">1.005 (0.984&#x2013;1.125)</td>
<td align="center" valign="top">0.745</td>
</tr>
<tr>
<td align="left" valign="top">sICH</td>
<td align="center" valign="top">2.885 (2.312&#x2013;3.519)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td align="center" valign="top">1.815 (1.515&#x2013;2.231)</td>
<td align="center" valign="top">&#x003C;0.001</td>
</tr>
<tr>
<td align="left" valign="top">DBP</td>
<td align="center" valign="top">1.022 (1.002&#x2013;1.041)</td>
<td align="center" valign="top">0.027</td>
<td align="center" valign="top">1.019 (0.985&#x2013;1.131)</td>
<td align="center" valign="top">0.095</td>
</tr>
<tr>
<td align="left" valign="top">Diabetes mellitus</td>
<td align="center" valign="top">1.562 (1.085&#x2013;2.246)</td>
<td align="center" valign="top">0.016</td>
<td align="center" valign="top">1.087 (0.512&#x2013;2.315)</td>
<td align="center" valign="top">0.865</td>
</tr>
<tr>
<td align="left" valign="top">LAA</td>
<td align="center" valign="top">Reference</td>
<td/>
<td align="center" valign="top">Reference</td>
<td/>
</tr>
<tr>
<td align="left" valign="top">SAO</td>
<td align="center" valign="top">0.602 (0.421&#x2013;0.859)</td>
<td align="center" valign="top">0.005</td>
<td align="center" valign="top">0.432 (0.223&#x2013;0.978)</td>
<td align="center" valign="top">0.062</td>
</tr>
<tr>
<td align="left" valign="top">CE</td>
<td align="center" valign="top">0.551 (0.331&#x2013;0.918)</td>
<td align="center" valign="top">0.022</td>
<td align="center" valign="top">1.032 (0.465&#x2013;2.180)</td>
<td align="center" valign="top">0.813</td>
</tr>
<tr>
<td align="left" valign="top">SOE</td>
<td align="center" valign="top">1.684 (0.514&#x2013;5.515)</td>
<td align="center" valign="top">0.389</td>
<td align="center" valign="top">2.296 (0.238&#x2013;6.371)</td>
<td align="center" valign="top">0.438</td>
</tr>
<tr>
<td align="left" valign="top">SUE</td>
<td align="center" valign="top">1.115 (0.513&#x2013;2.422)</td>
<td align="center" valign="top">0.784</td>
<td align="center" valign="top">0.452 (0.051&#x2013;1.957)</td>
<td align="center" valign="top">0.219</td>
</tr>
<tr>
<td align="left" valign="top">FBG</td>
<td align="center" valign="top">1.178 (1.123&#x2013;1.235)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">TG</td>
<td align="center" valign="top">2.142 (1.847&#x2013;2.485)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">HDL-C</td>
<td align="center" valign="top">0.442 (0.241&#x2013;0.810)</td>
<td align="center" valign="top">0.008</td>
<td/>
<td/>
</tr>
<tr>
<td align="left" valign="top">TC</td>
<td align="center" valign="top">1.023 (0.999&#x2013;1.130)</td>
<td align="center" valign="top">0.04</td>
<td align="center" valign="top">1.012 (0.982&#x2013;1.113)</td>
<td align="center" valign="top">0.996</td>
</tr>
<tr>
<td align="left" valign="top">TyG index</td>
<td align="center" valign="top">3.879 (3.029&#x2013;4.968)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td align="center" valign="top">2.015 (1.964&#x2013;2.049)</td>
<td align="center" valign="top">0.015</td>
</tr>
<tr>
<td align="left" valign="top">TG/HDL-C</td>
<td align="center" valign="top">1.847 (1.637&#x2013;2.084)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td align="center" valign="top">1.542 (1.160&#x2013;2.049)</td>
<td align="center" valign="top">0.004</td>
</tr>
</tbody>
</table>
</table-wrap>
<fig position="float" id="fig3">
<label>Figure 3</label>
<caption>
<p>Binary logistic analysis of independent risk factors associated with post-thrombolysis END.</p>
</caption>
<graphic xlink:href="fneur-15-1421655-g003.tif"/>
</fig>
<table-wrap position="float" id="tab3">
<label>Table 3</label>
<caption>
<p>Association of the TyG index and TG/HDL-C ratio with post-thrombolysis END.</p>
</caption>
<table frame="hsides" rules="groups">
<thead>
<tr>
<th align="left" valign="top">Variable</th>
<th align="center" valign="top">OR (95% CI)</th>
<th align="center" valign="top"><italic>P</italic></th>
<th align="center" valign="top">Adjusted OR (95% CI)<xref ref-type="table-fn" rid="tfn1"><sup>a</sup></xref></th>
<th align="center" valign="top"><italic>P</italic></th>
</tr>
</thead>
<tbody>
<tr>
<td align="left" valign="top" colspan="5"><bold>TyG index binary classification</bold></td>
</tr>
<tr>
<td align="left" valign="top">T1</td>
<td align="center" valign="top">Reference</td>
<td/>
<td align="center" valign="top">Reference</td>
<td/>
</tr>
<tr>
<td align="left" valign="top">T2</td>
<td align="center" valign="top">2.650 (1.882&#x2013;3.731)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td align="center" valign="top">2.32 1(2.013&#x2013;2.954)</td>
<td align="center" valign="top">0.004</td>
</tr>
<tr>
<td align="left" valign="top" colspan="5"><bold>TG/HDL-C binary classification</bold></td>
</tr>
<tr>
<td align="left" valign="top">T1</td>
<td align="center" valign="top">Reference</td>
<td/>
<td align="center" valign="top">Reference</td>
<td/>
</tr>
<tr>
<td align="left" valign="top">T2</td>
<td align="center" valign="top">2.570 (1.828&#x2013;3.614)</td>
<td align="center" valign="top">&#x003C;0.001</td>
<td align="center" valign="top">2.085 (1.514&#x2013;2.873)</td>
<td align="center" valign="top">0.02</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<p>TyG index: T1&#x2009;&#x003C;&#x2009;7.17; T2&#x2009;&#x2265;&#x2009;7.17; TG/HDL-C: T1&#x2009;&#x003C;&#x2009;1.26; T2&#x2009;&#x2265;&#x2009;1.26.</p>
<fn id="tfn1"><label>a</label><p>Model: adjusted for DBP, stroke subtype, and TC.</p></fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec14">
<title>ROC curve analysis to determine the overall ability to discriminate post-thrombolysis END</title>
<p>We used ROC curves to assess the overall ability of the TyG index and TG/HDL-C ratio to discriminate post-thrombolysis END (<xref ref-type="fig" rid="fig4">Figure 4</xref>). The TyG index&#x2019;s area under the curve (AUC) for determining post-thrombolysis END was 0.704 (95% CI, 0.678&#x2013;0.730; <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and the cut-off value was 7.78, with a sensitivity of 53.1% and a specificity of 85.3%. For the TG/HDL-C ratio, the AUC was 0.674 (95% CI, 0.647&#x2013;0.701; <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and the cut-off value was 2.94, with a sensitivity of 36.3% and a specificity of 93.9%. In addition, we conducted an ROC curve analysis to assess the discriminatory power of the TyG index and TG/HDL-C ratio combination in distinguishing between the END group and the non-END group. The AUC for the combination of the TyG index and TG/HDL-C ratio was 0.755 (95% CI: 0.730&#x2013;0.780, <italic>p</italic>&#x2009;&#x003C;&#x2009;0.001), and the cut-off value was 0.18, with a sensitivity of 62.6% and a specificity of 81.6%.</p>
<fig position="float" id="fig4">
<label>Figure 4</label>
<caption>
<p>ROC analysis revealed that the TyG index, TG/HDL-C ratio, and TyG index and the TG/HDL-C ratio exhibited respectable post-thrombolysis END discriminating power, with AUC values of 0.704, 0.674, and 0.755, respectively.</p>
</caption>
<graphic xlink:href="fneur-15-1421655-g004.tif"/>
</fig>
</sec>
</sec>
<sec sec-type="discussion" id="sec15">
<title>Discussion</title>
<p>In our research, 179 patients (15.08%) experienced post-thrombolysis END, and the proportion was consistent with the results of previous studies (<xref ref-type="bibr" rid="ref5">5</xref>, <xref ref-type="bibr" rid="ref9">9</xref>, <xref ref-type="bibr" rid="ref29">29</xref>). The results we obtained offer a number of fresh insights. First, we found that the TyG index and TG/HDL-C ratio in AIS patients with END were greater than those in AIS patients with non-END. Second, the binary logistic regression model indicated that the TyG index and TG/HDL-C ratio were independent factors for post-thrombolysis END. Finally, we employed ROC curves to test the overall ability of the TyG index and TG/HDL-C ratio to discriminate post-thrombolysis END. Together, these findings provide evidence that a higher TyG index and TG/HDL-C ratio are associated with post-thrombolysis END.</p>
<p>An increasing amount of research has demonstrated a relationship between the TyG index and the prognosis of AIS patients. The TyG index is related to arterial stiffness (<xref ref-type="bibr" rid="ref30">30</xref>) and poor outcomes in cardiovascular and cerebrovascular diseases (<xref ref-type="bibr" rid="ref31">31</xref>, <xref ref-type="bibr" rid="ref32">32</xref>). According to multicenter observational research, a higher TyG index was associated with 90-day unfavorable functional outcomes in AIS patients after thrombolysis (<xref ref-type="bibr" rid="ref18">18</xref>). Additionally, the TyG index was related to a greater risk of in-hospital mortality in patients with severe stroke (<xref ref-type="bibr" rid="ref33">33</xref>) and early stroke recurrence (<xref ref-type="bibr" rid="ref34">34</xref>). In our study, the TyG index was significantly greater in the post-thrombolysis END group than in the non-END group. A binary logistic regression model indicated that the TyG index was an independent factor for post-thrombolysis END. In addition, when the TyG index was used as a categorical variable, after adjusting for confounding factors, our data revealed that a higher TyG index was associated with a greater probability of developing post-thrombolysis END, which is consistent with the findings of previous studies (<xref ref-type="bibr" rid="ref9">9</xref>). These findings may indicate that the TyG index is a biomarker for END.</p>
<p>The associations between post-thrombolysis END and the TyG index can be explained by several mechanisms. First, IR may cause excessive platelet activation, exacerbate endothelial dysfunction, and cause biochemical imbalances (<xref ref-type="bibr" rid="ref35">35</xref>, <xref ref-type="bibr" rid="ref36">36</xref>). Second, IR induces a variety of metabolic disorders, which promote atherosclerotic plaque rupture, leading to thrombosis (<xref ref-type="bibr" rid="ref37">37</xref>). Third, IR can exacerbate oxidative stress, which can result in the accumulation of reactive oxygen species and mitochondrial dysfunction. Finally, IR can increase matrix metalloproteinase-9 activity, which exacerbates ischemia, reperfusion damage, and the inflammatory response (<xref ref-type="bibr" rid="ref38">38</xref>, <xref ref-type="bibr" rid="ref39">39</xref>).</p>
<p>Based on a prior study, in the hypertensive population, an increased TG/HDL-C ratio was predictive of increased risk and advanced development of arterial stiffness (<xref ref-type="bibr" rid="ref40">40</xref>). According to a longitudinal study, the TG/HDL-C ratio may be a significant and distinct biomarker for predicting cardiovascular disease outcomes and progression (<xref ref-type="bibr" rid="ref41">41</xref>). Furthermore, data from the UK Biobank cohort revealed that an elevated TG/HDL-C ratio was associated with a greater risk of cardiovascular disease (<xref ref-type="bibr" rid="ref13">13</xref>). However, the correlation between the TG/HDL-C ratio and post-thrombolysis END in patients with AIS remains unclear. In this study, in the post-thrombolysis END group, the TG/HDL-C ratio was significantly greater than that in the non-END group. Furthermore, the TG/HDL-C ratio was identified as an independent factor for post-thrombolysis END, after adjustment for potential confounders. Employing the TG/HDL-C ratio as a categorical variable, our results demonstrated that a higher TG/HDL-C ratio was related to an increased risk of post-thrombolysis END development. Our findings expand the understanding of the function of the TG/HDL-C ratio in cerebrovascular disease and provide fresh perspectives on therapeutic approaches.</p>
<p>In our study, the proportion of sICH in the END group was 26.82% (48/179), which is similar to the study by Yu et al. (<xref ref-type="bibr" rid="ref27">27</xref>), and the overall percentage of patients with sICH was 4.04% (48/1,187) for all AIS patients after thrombolysis, which is consistent with earlier studies (<xref ref-type="bibr" rid="ref1">1</xref>, <xref ref-type="bibr" rid="ref28">28</xref>). In this research, sICH was identified as an independent risk factor for post-thrombolysis END after adjustment for all potential confounders, which is similar to the earlier study (<xref ref-type="bibr" rid="ref42">42</xref>). Age and the NIHSS score were also important factors for END (<xref ref-type="bibr" rid="ref43">43</xref>). However, there was no significant correlation between age, the NIHSS score, and post-thrombolysis END in our research. We believe that the variances in the ethnicity of the research populations, the sample sizes, the medication status, and the severity of the condition may be the causes of the discrepancies between various studies.</p>
<p>We employed ROC curves to test the overall ability of the TyG index and TG/HDL-C ratio to discriminate post-thrombolysis END. Our research revealed that the TyG index and TG/HDL-C ratio had the ability to distinguish patients in the END group from those in the non-END group. The TyG index is more discriminative than the TG/HDL-C ratio, indicating that the TyG index might be a valuable instrument for predicting post-thrombolysis END. Moreover, our study revealed that the combination of the TyG index and TG/HDL-C ratio exhibited superior discriminatory power for post-thrombolysis END, with an AUC of 0.755. This value surpassed that of the individual markers, suggesting that the combination of these two indicators may be more beneficial in predicting post-thrombolysis END.</p>
<p>The limitations of this study are as follows: (1) This was a cross-sectional study with only Chinese patients receiving intravenous thrombolysis; thus, potential inherent biases exist. As a result, our findings need to be verified in non-Chinese populations, and future research may require larger-scale longitudinal cohort studies. (2) Owing to realistic limitations resulting from our clinical context, our research team did not employ a direct IR assessment tool. (3) Given that IR detection is reliant on time during the onset of AIS, early measurement may have overestimated the prevalence of IR. Future research needs to consider the effects of peripheral blood markers.</p>
</sec>
<sec sec-type="conclusions" id="sec16">
<title>Conclusion</title>
<p>In conclusion, our study suggests that the TyG index and TG/HDL-C ratio can be used as prognostic factors to predict post-thrombolysis END. Additionally, the combination of the TyG index and TG/HDL-C ratio may provide greater predictive value. However, more research is needed to confirm these findings and clarify the pathophysiology of post-thrombolysis END.</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="sec17">
<title>Data availability statement</title>
<p>The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec sec-type="ethics-statement" id="sec18">
<title>Ethics statement</title>
<p>The studies involving humans were approved by the Ethics Committee of Changsha Central Hospital and Hunan Province&#x2019;s Second People&#x2019;s Hospital. The studies were conducted in accordance with the local legislation and institutional requirements. Written informed consent for participation was not required from the participants or the participants&#x2019; legal guardians/next of kin in accordance with the national legislation and institutional requirements.</p>
</sec>
<sec sec-type="author-contributions" id="sec19">
<title>Author contributions</title>
<p>MD: Writing &#x2013; original draft, Methodology, Investigation, Formal analysis, Data curation. KS: Writing &#x2013; original draft, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation. WX: Writing &#x2013; original draft, Methodology, Funding acquisition, Formal analysis, Data curation. GH: Writing &#x2013; review &#x0026; editing, Supervision, Investigation, Formal analysis. JH: Writing &#x2013; review &#x0026; editing, Validation, Methodology, Formal analysis. HX: Writing &#x2013; review &#x0026; editing, Formal analysis, Data curation, Conceptualization. NZ: Writing &#x2013; review &#x0026; editing, Methodology, Formal analysis. SC: Writing &#x2013; review &#x0026; editing, Validation, Formal analysis, Conceptualization. GX: Writing &#x2013; review &#x0026; editing, Methodology, Data curation. YT: Writing &#x2013; review &#x0026; editing, Investigation, Data curation. DZ: Writing &#x2013; review &#x0026; editing, Data curation. ZW: Writing &#x2013; review &#x0026; editing, Validation, Supervision, Methodology, Formal analysis, Data curation, Conceptualization. FL: Writing &#x2013; review &#x0026; editing, Validation, Supervision, Resources, Project administration, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation, Conceptualization.</p>
</sec>
<sec sec-type="funding-information" id="sec20">
<title>Funding</title>
<p>The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was funded by the Natural Science Foundation of Hunan Province (grant no. 2023JJ40072), the Science and Technology Planning Project of Hunan Health Committee (grant no. D202303076376), the Medical Clinical Research 4310 Project of University of South China (grant no. KJZX2021078), the Scientific Research Project of Hunan Provincial Health Commission (No. 202103070273), the Changsha Science and Technology Project (No. kzd2001068), and the Funded Project of the Affiliated Changsha Central Hospital, University of South China (No. YNKY202211).</p>
</sec>
<sec sec-type="COI-statement" id="sec21">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="disclaimer" id="sec22">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<sec sec-type="supplementary-material" id="sec23">
<title>Supplementary material</title>
<p>The Supplementary material for this article can be found online at: <ext-link xlink:href="https://www.frontiersin.org/articles/10.3389/fneur.2024.1421655/full#supplementary-material" ext-link-type="uri">https://www.frontiersin.org/articles/10.3389/fneur.2024.1421655/full#supplementary-material</ext-link></p>
<supplementary-material xlink:href="Table_1.DOCX" id="SM1" mimetype="application/vnd.openxmlformats-officedocument.wordprocessingml.document" xmlns:xlink="http://www.w3.org/1999/xlink"/>
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