AUTHOR=Jackson Louis , Brethenoux Caroline , DeLuca Alyssa , Pesa Jacqueline , Choudhry Zia , Furey Patrick , Alvarez Rosario , Gonzalez Laura , Lorenzo Alex , Govindarajan Raghav , Anderson Ashley E. L. TITLE=AI-based thematic exploration to understand patients with myasthenia gravis by serological subtype JOURNAL=Frontiers in Neurology VOLUME=Volume 16 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2025.1576405 DOI=10.3389/fneur.2025.1576405 ISSN=1664-2295 ABSTRACT=ObjectiveMyasthenia gravis (MG) is challenging to diagnose and appropriate treatment is informed by serological versus diagnostic testing. Digital conversations can reveal insights into patient perceptions and concerns that may differ across autoantibody subtypes. The purpose of this study was to describe MG-related sentiments, barriers, and drivers by describing digital conversations by serostatus/serotype.MethodsUS-based public-domain conversations focusing on MG and posted within topical sites, message boards, social networks, and blogs from August 2022 to August 2023 were mined. Content contributors self-identified by MG serostatus (seropositive/seronegative/AChR+/MuSK+/LRP4+) within the conversations or on public profiles. Advanced search techniques and AI-powered algorithms were used to extract/organize data by topics into a large, unstructured dataset. Natural language processing identified frequent topics, sentiments, mindsets, and drivers/barriers to treatment.ResultsIn total, 8,784 conversations were mined from seropositive patients and/or their caregivers (including 1,058 AChR+, 589 MuSK+, and 537 LRP4+) and 2,261 were mined from seronegative patients and/or their caregivers. All conversations revealed an uncertain mindset, with a struggling mindset more prevalent among seronegative posts and ~40% of seropositive posts deemed utilitarian/indomitable. Positive drivers of sentiment were not seen for any subgroup. Negative drivers centered on misdiagnosis and symptoms more frequently in seronegative posts, and on impact on life in seropositive posts. Instability of symptom control in all subgroups impacted quality of life.ConclusionConversations revealed the burden of misdiagnosis for the seronegative group, negative impact on life for the seropositive group, and overall difficulty of the MG diagnostic journey. Digital conversations across MG serostatus subgroups related to the patient/caregiver experience of living with MG might provide useful insights for healthcare professionals seeking to improve patient care.