AUTHOR=Evertsson Björn , Remahl Ingela Nilsson , Hietala Max Albert , Finn Anja TITLE=Glial fibrillary acidic protein in cerebrospinal fluid in humans is sensitive to various pre-analytical conditions: possible explanation and solution JOURNAL=Frontiers in Neurology VOLUME=Volume 16 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2025.1627405 DOI=10.3389/fneur.2025.1627405 ISSN=1664-2295 ABSTRACT=Although glial fibrillary acidic protein (GFAP) has potential as a biomarker in cerebrospinal fluid, it is rarely used in clinical diagnosis due to high variability, low reliability, and poor reproducibility of results. Cerebrospinal fluid (CSF) was collected from patients (n = 167) at two sites at the Department of Neurology. CSF was sampled in various volumes in both 10 mL polypropylene (PP) tubes and small, filled, sealed tubes of ≤2.0 mL (microtubes) for the comparison of GFAP concentrations. The influence of pH, sample volumes during storage and transport of CSF, under different temperatures, was tested to identify the losses and increase the possibilities of replicating data for GFAP. Concentrations of GFAP were measured by a sandwich ELISA. Exposure to air, agitation, and open-close cycles increased pH and lowered CO2. Compared to corresponding small filled sealed tubes, routine samples stored at −20°C showed 4–30% lower concentrations of GFAP. The loss increased further at lower volumes (< 0.5 mL). A significant difference in GFAP concentrations was seen in samples taken offsite (loss 42%) and onsite (loss 24%) compared to corresponding microtubes. Concentrations of GFAP remained stable in the microtubes, at 2–8°C and at RT for up to 3 weeks. GFAP in CSF is highly sensitive to changes in pH and dependent on adequate volumes for the best results. By avoiding exposure to air and agitation, we were able to stabilize GFAP concentrations in CSF by using small, filled, sealed tubes (microtubes). This handling could have impact on other biomarkers.