AUTHOR=Kangussu Lucas M. , Melo-Braga Marcella Nunes , de Souza Lima Bruna Soares , Santos Robson A. S. , de Andrade Hélida Monteiro , Campagnole-Santos Maria José TITLE=Angiotensin-(1-7) Central Mechanisms After ICV Infusion in Hypertensive Transgenic (mRen2)27 Rats JOURNAL=Frontiers in Neuroscience VOLUME=Volume 15 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2021.624249 DOI=10.3389/fnins.2021.624249 ISSN=1662-453X ABSTRACT=Hypertensive rats subjected to chronic intracerebroventricular (ICV) infusion of angiotensin-(1-7) presented attenuation of arterial hypertension, improvement of baroreflex sensitivity, restoration of cardiac autonomic balance and a shift of cardiac renin-angiotensin system (RAS) balance towards Ang-(1-7)/Mas receptor. In this study, we investigated central mechanisms that could be involved in these effects, including inflammatory mediators and the expression/activity of the RAS components in hypertensive rats subjected to chronic treatment with Ang-(1-7). Furthermore, we performed a proteomic analysis to evaluate differentially regulated proteins in the hypothalamus of these animals. Sprague Dawley (SD) and transgenic (mRen2)27 hypertensive rats (TG) were subjected to 14 days of ICV infusion with Ang-(1-7) (200 ng/h) or 0.9% sterile saline (0.5 μl/h) through osmotic mini-pumps. Our results showed that treatment with Ang-(1-7) modulated inflammatory cytokines, decreasing the level of TNF-α while increasing the anti-inflammatory IL-10. Moreover, we showed a reduction in ACE activity, gene expression of the angiotensin II receptor AT1, iNOS after Ang-(1-7) treatment. Finally, our proteomic data suggested an anti-inflammatory mechanism of Ang-(1-7) toward the ROS modulators Uchl1 and Prdx1.