AUTHOR=Ni Shuangfei , Luo Zixiang , Fan Yonggang , Zhang Weixin , Peng Wei , Zhang Huafeng 

TITLE=Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice

JOURNAL=Frontiers in Neuroscience

VOLUME=Volume 16 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2022.904573

DOI=10.3389/fnins.2022.904573

ISSN=1662-453X

ABSTRACT=M6A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m6A modification to post-SCI damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA m6A levels were decreased following SCI. Then, GO and KEGG analyses were conducted to demonstrate the potential function of differential m6A-tagged transcripts and the altered transcripts with differential m6A levels. Additionally, we found that the m6A “writer”, mettl3, significantly decreased after SCI in mice. The immunostaining validated that the expression of mettl3 mainly changed in GFAP+ or Iba-1+ cells. Together, this study shows the alteration of m6A modification following SCI in mice, which might contribute to the pathophysiology in the spinal cord after trauma.