AUTHOR=Tao Jing-Wei , Fan Xiao , Zhou Jing-Ya , Huo Lu-Yao , Mo Yan-Jun , Bai Hui-Zhong , Zhao Yi , Ren Jing-Pei , Mu Xiao-Hong , Xu Lin 

TITLE=Granulocyte colony-stimulating factor effects on neurological and motor function in animals with spinal cord injury: a systematic review and meta-analysis

JOURNAL=Frontiers in Neuroscience

VOLUME=Volume 17 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2023.1168764

DOI=10.3389/fnins.2023.1168764

ISSN=1662-453X

ABSTRACT=Abstract
Background: Spinal cord injury (SCI) is a serious neurological injury for which no effective treatment exists. Granulocyte colony-stimulating factor (G-CSF) is used to treat auto-bone marrow transplantation, chemotherapy-induced granulocytopenia, Acquired Immune Deficiency Syndrome (AIDS), etc. Recent research has revealed the potential application of G-CSF on neuroprotective effectiveness. In central nervous system illnesses, G-CSF may be used to alleviate neuronal injury.
Objective: To investigate G-CSF effects on Basso, Beattie, and Bresnahan (BBB) scale score, Inclined plane test, electrophysiologic exam; quantitative analysis of TUNEL-positive cell numbers; quantitative analysis of glial fibrillary acidic protein (GFAP) immunostaining image in animal models of SCI.
Methods: We searched Web of Science, PubMed, and Embase databases for all articles on G-CSF intervention with animal models of SCI reported before November 2022. Twenty studies were included by the inclusion criteria.
Results: Results revealed that G-CSF intervention could improve the BBB scale score in both groups at 3, 7, 14, 28, and 35 days [At 35 days Weighted mean differences (WMD) = 2.4, 95% CI: 1.92- 2.87, P < 0.00001, I2 = 69%]; Inclined plane test score; Electrophysiologic exam; Quantitative analysis of TUNEL-positive cell numbers; Quantitative analysis of GFAP immunostaining image in animal models of SCI. Subgroup analysis revealed that treatment with normal saline and phosphate-buffered saline and no treatment resulted in significantly different neurological function effectiveness compared to the G-CSF therapy. SD rats and Wistar rats with SCI resulted in significant neurological function effectiveness. C57BL/6 mice showed no difference in the final effect. The T9–T10 or T10 segment injury model and T8–T9 or T9 segment injury model resulted in significant neurological function effectiveness. The BBB score data showed no clear funnel plot asymmetry. We had not found any bias in the analysis result (Egger’s test, P = 0.42). In our network meta-analysis, the SUCRA ranking showed that 15mg/kg-20mg/kg was an optimal dose for long-term efficacy. 
Conclusion: Our meta-analysis suggested that G-CSF therapy could enhance the recovery of motor activity and has a specific neuroprotective effect on SCI animal models.
Systematic review registration: PROSPERO, identifier: CRD42023388315.