AUTHOR=Chu Yanhao , Wu Yaqi , Jia Shilin , Xu Ke , Liu Jinyue , Mai Lijia , Fan Wenguo , Huang Fang 

TITLE=Single-nucleus transcriptome analysis reveals transcriptional profiles of circadian clock and pain related genes in human and mouse trigeminal ganglion

JOURNAL=Frontiers in Neuroscience

VOLUME=Volume 17 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2023.1176654

DOI=10.3389/fnins.2023.1176654

ISSN=1662-453X

ABSTRACT=Clinical studies have revealed the existence of circadian rhythms in pain intensity and treatment response for chronic pain, including orofacial pain. The circadian clock genes in the peripheral ganglia are involved in pain information transmission by modulating the synthesis of pain mediators. However, the expression and distribution of clock genes and pain-related genes in different cell types within the trigeminal ganglia, the primary station of orofacial sensory transmission, are not yet fully understood. In this study, data from the normal trigeminal ganglion in the Gene Expression Omnibus (GEO) database were used to identify cell types and neuron subtypes within the human and mouse trigeminal ganglion by single nucleus RNA sequencing analysis. Our findings provide comprehensive transcriptional profiles of core clock genes, pain-related genes, melatonin-related genes, and opioid-related genes in different cell types of the trigeminal ganglia. These results serve as a valuable resource for exploring the molecular mechanisms underlying pain rhythms and orofacial messaging.