AUTHOR=Ding Jingwei , Shibata Shigenobu , Kubo Tatsuhiko , Tahara Yu 

TITLE=Time-dependent cortical responses to siesta disruption in male mice

JOURNAL=Frontiers in Neuroscience

VOLUME=Volume 19 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2025.1613747

DOI=10.3389/fnins.2025.1613747

ISSN=1662-453X

ABSTRACT=IntroductionSiestas, or daytime naps, play a critical role in relieving sleep pressure and maintaining physiological balance. However, the effects of siesta disruption remain largely unexplored.MethodsIn this study, we disrupted the natural siesta period (ZT20–23) through daily bedding changes for 2 weeks and examined its effects on overall stress levels, sleep architecture, behavior, and transcriptional responses in the frontal cortex.ResultsSiesta disruption during the late dark phase led to increased core body temperature, locomotor activity, and wakefulness, while having minimal effects on subsequent light phase sleep patterns, behavioral performance, or serum stress markers. Transcriptomic analysis at ZT0 and ZT12 revealed distinct time dependent responses: ZT0 was associated with the activation of stress-related and homeostatic pathways, whereas ZT12 showed enrichment of genes related to neuronal structure and intracellular transport.DiscussionThese findings suggest that even short-term siesta disruption induces mild but phase-specific cortical adaptations, involving early stress mitigation at ZT0 followed by synaptic remodeling at ZT12.