AUTHOR=Jia Wei , Zhang Rong , Zhu Zhenbao , Shi Lin 

TITLE=A High-Throughput Comparative Proteomics of Milk Fat Globule Membrane Reveals Breed and Lactation Stages Specific Variation in Protein Abundance and Functional Differences Between Milk of Saanen Dairy Goat and Holstein Bovine

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 8 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2021.680683

DOI=10.3389/fnut.2021.680683

ISSN=2296-861X

ABSTRACT=Large variations on the bioactivities and composition of milk fat globule membrane (MFGM) proteins were observed between Saanen dairy goat and Holstein bovine at various lactation periods. In the present study, 331, 250, 182, and 248 MFGM proteins were characterized in colostrum and mature milk for the two species by Q-Orbitrap HRMS-based proteomics techniques. KEGG pathway analyses displayed that differential expressed proteins in colostrum involved in galactose metabolism and adipogenesis pathway, and the differential expressed proteins in mature milk associated with lipid metabolism and PPAR signaling pathway. These results indicated that the types and functions of MFGM proteins in goat and bovine milk were different and goat milk had better function of fatty acid metabolism and glucose homeostasis, which can enhance our understanding of MFGM proteins in these two species across different lactation periods, provide significant information for the study of lipid metabolism and glycometabolism of goat milk.