AUTHOR=Li Minjie , Wang Hongping , Li Simeng , Chen Xueying , Jin Maojun , Shao Hua , Wang Jing , Jin Fen 

TITLE=High-throughput analysis of polyethoxylated tallow amine homologs in citrus using a modified QuEChERS-HILIC-MS method

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.1061195

DOI=10.3389/fnut.2022.1061195

ISSN=2296-861X

ABSTRACT=A new method is described based on ultraperformance liquid chromatography (UPLC) with electrospray mass spectrometry detection for comprehensive quantitative analysis of 66 polyethoxylated tallow amines (POE-Tallowamine) homologs in citrus. Efficient separation, reduced band broadening, and high sensitivity were achieved by employing a acetonitrile-aqueous solution containing 10 mM ammonium formate gradient on a HILIC column with a modified QuEChERS method. Quantitative accuracy and precision of the method were improved by the use of matrix-matched calibration standards. The average recoveries of the POE-Tallowamine homologs at different spiked concentrations ranged from 71.9 to 112%, with RSDs < 16.6%. The limits of detection (LODs) and limits of quantification (LOQs) for POE-Tallowamine homologs were 0.01–2.57 and 0.03–8.58 μg/kg, respectively. The method was successfully applied to determine POE-Tallowamine in citrus samples from Chinese typical regions in 2021. The POE-Tallowamine was detected in all 54 samples, and the highest concentration (143 μg/kg) of POE-Tallowamine was found in Jelly orange from Zhejiang province.