AUTHOR=Pagliari Stefania , Giustra Chiara Maria , Magoni Chiara , Celano Rita , Fusi Paola , Forcella Matilde , Sacco Grazia , Panzeri Davide , Campone Luca , Labra Massimo 

TITLE=Optimization of ultrasound-assisted extraction of naturally occurring glucosinolates from by-products of Camelina sativa L. and their effect on human colorectal cancer cell line

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.901944

DOI=10.3389/fnut.2022.901944

ISSN=2296-861X

ABSTRACT=Food waste generated by small and medium agro-industrial enterprise require appropriate management and valorisation in order to decrease the environmental problems and recover high value products, respectively. In this study, the Camelina sativa seed by-product was used as source of glucosinolates. To begin, the chemical profile of the extract obtained using the ISO procedure was performed by UPLC-HRMS/MS analysis. In addition, an extraction method based on the ultrasound assisted extraction was developed as alternative and green method to recover glucosinolates. The main parameters that affect the extraction efficiency were optimized using a response surface design. Under the optimized conditions, the USA extract showed an improvement of the extraction yield with a reduction of organic solvent amount than those obtained using the ISO procedure. Finally, the USAE was purified and tested on human colorectal cancer cell lines showing promising results.