AUTHOR=Zhang Nan , Yang Bing , Mao Kemin , Liu Yuwei , Chitrakar Bimal , Wang Xianghong , Sang Yaxin 

TITLE=Comparison of structural characteristics and bioactivity of Tricholoma mongolicum Imai polysaccharides from five extraction methods

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.962584

DOI=10.3389/fnut.2022.962584

ISSN=2296-861X

ABSTRACT=Tricholoma mongolicum Imai is an edible fungus rich in various health promoting compounds, such as polysaccharides, polypeptides, polyunsaturated fatty acids etc., among them polysaccharides have got more attention in recent research trends. This study explored the extraction of polysaccharides from T. mongolicum Imai by five extract methods including hot water extraction, ultrasound extraction, enzyme-assisted extraction, 0.1 M HCL extraction and 0.1 M NaOH extraction. The effects of these extraction methods on the yield, chemical structure, apparent morphology, antioxidant activities of Tricholoma mongolicum Imai polysaccharides (TMIPs) were investigated in this study. The results of High performance gel permeation chromatography (HPGPC) and scanning electron microscopy (SEM) showed that different extraction methods had significant effects on the molecular weight and morphology of TMIPs. The results of Fourier transform infrared (FT-IR), X-Ray Diffraction (XRD) and thermogravimetric analysis showed that the extraction methods had no significant difference on functional groups, crystal structure and thermal stability of TMIPs. The antioxidant activity of TMIPs extracted by ultrasound extraction was more prominent among the five polysaccharides, which might be related to the large number of low molecular weight components in molecular weight distribution.