AUTHOR=Wang Ruobing , Gong Min , Liu Yang , Zhu Weiran , Zhang Kai , Zhao Yidi , Yin Chen , Liu Yuan , Wang Jian , Wan Yuping 

TITLE=Development of a highly sensitive colloidal gold semiquantitative method for the determination of difenoconazole residues in citrus

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1341219

DOI=10.3389/fnut.2024.1341219

ISSN=2296-861X

ABSTRACT=Difenoconazole(DIFE) is a common pesticide used in citrus cultivation, excessive intake can cause neurological damage to the organism, and the existing colloidal gold immunochromatographic test strips cannot meet the requirements for the detection of citrus samples Difenoconazole was prepared based on the colloidal gold immunochromatographic technique (GICT) and its application in citrus samples was investigated; (2) With colloidal gold (CG) as the probe, the optimization of GICT parameters and the determination of reaction method, the immunochromatographic test strips for the detection of DIFE in citrus was developed, and the limit of detection (LOD), specificity, accuracy and stability of the test strips were verified; (3) The results manifested that the visual detection limit of the prepared colloidal gold immunochromatographic test strips was 0.2 mg/kg and the quantitative range was 0.06-0.6 mg/kg, and the test strips could specifically identify DIFE and had no cross reaction with other common triazole pesticides, The detection method established in this study was verified by GC-MS method, and the detection results achieved good consistency (R 2 >0.98); (4) The conclusion is that the test strips developed in this study have good performance and can be used for highly sensitive detection of citrus samples.