AUTHOR=Afolabi Oladele A. , Akhigbe Tunmise M. , Hammed Sodiq. O. , Hamed Moses A. , Ekundina Victor O. , Ajike Richard A. , Alabi Babatunde A. , Akhigbe Roland E. 

TITLE=Moringa oleifera-based feed supplement protects against renal ischaemia/reperfusion injury via downregulation of Bax/caspase 3 signaling

JOURNAL=Frontiers in Nutrition

VOLUME=Volume 11 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1396864

DOI=10.3389/fnut.2024.1396864

ISSN=2296-861X

ABSTRACT=This study was designed to assess whether or not Moringa oleifera-based feed supplement could prevent ischaemia/reperfusion (I/R)-induced renal injury. Renal I/R was induced by occluding the right renal artery for 30 minutes followed by a 2-hour reperfusion. Renal I/R led to increased absolute renal weight and renal organo-somatic weight index. Renal I/R also caused distortion of renal histoarchitecture and impaired renal function evidenced by elevated serum creatinine and blood urea nitrogen. In addition, renal I/R significantly elevated renal levels of hydrogen peroxide, MDA, and advanced oxidation protein products, but suppressed the levels of reduced glutathione, protein thiol, and non-protein thiol, and the activities of superoxide dismutase and glutathione peroxidase. In addition, renal I/R up-regulated myeloperoxidase activity and the renal levels of NO, TNF-α, and IL-6. Renal I/R also up-regulated Bax and caspase 3 expression in the kidney. Furthermore, I/R-driven structural and biochemical alterations were markedly inhibited by Moringa oleifera-based feed supplement. These results suggest that Moringa oleifera-based feed supplement may preserve the gross and histoarchitectural integrity of the kidney as well as renal function via downregulation of Bax/caspase 3 signaling by targeting oxidative stress, inflammation and apoptosis in the kidney of I/R rat.