AUTHOR=Cui Zhanding , Liu Jinlong , Wang Jinlong , Zhang Jing , Cao Yimei , Li Kun , Zhao Zhixun , Yuan Hong , Bai Xingwen , Ma Xueqing , Li Pinghua , Fu Yuanfang , Bao Huifang , Li Dong , Zhang Qiang , Liu Zaixin , Li Kai , Xu Tong , Lu Zengjun TITLE=Formononetin and mizoribine inhibit Porcine Reproductive and Respiratory Syndrome Virus replication in vitro JOURNAL=Frontiers in Nutrition VOLUME=Volume 11 - 2024 YEAR=2025 URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2024.1501685 DOI=10.3389/fnut.2024.1501685 ISSN=2296-861X ABSTRACT=This study delves into the antiviral efficacy of Formononetin (FMN) and Mizoribine (MZR) against the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a virus with a considerable economic impact and a current void in effective treatments. FMN and MZR were found to inhibit various PRRSV strains in vitro, predominantly in the early stages of viral infection. Noteworthy was the observation of their synergistic effects when combined with Ribavirin. The study underscores the antiviral potential of FMN and MZR, particularly emphasizing their low cytotoxicity at specific concentrations. These results position FMN and MZR as promising antiviral agents against PRRSV, underscoring their low cytotoxicity and efficacy in early-stage viral inhibition. Such findings pave the way for their potential inclusion in future PRRSV management strategies.