AUTHOR=Wang Yafei , Ma Ronghui , Wei Jianlin , Fu Xiaoyan , Zhang Shanshan , Zhao Zichao , Lin Haitao , Xu Yu , Tan Deshui , Gao Xibao , Liu Yumin TITLE=Enhancing micronutrient bioavailability in wheat grain through organic fertilizer substitution JOURNAL=Frontiers in Nutrition VOLUME=Volume 12 - 2025 YEAR=2025 URL=https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1559537 DOI=10.3389/fnut.2025.1559537 ISSN=2296-861X ABSTRACT=The effect of organic fertilizer substitution (OFS) on crop micronutrients often varies due to differences in environmental conditions, soil types, and nutrient status. This study aims to evaluate the effects of OFS on wheat grain micronutrients and bioavailability across five sites in Shandong Province from 2021 to 2022. All experimental sites included five common treatments: control, traditional farming, optimized practices, and 15 and 30% OFS for chemical nitrogen. The results revealed regional variation in wheat yield; the average wheat yield was 9.06 Mg ha−1, and the highest yield was 9.58 Mg ha−1 in the 15%OF treatment. No significant differences in soil micronutrient availability were observed. Compared to the control, the OFS treatments exhibited significant increases in grain Fe (24.69%) and Zn (19.19%) contents. The OFS treatments significantly increased Fe and Zn bioavailability by reducing the PA/Fe and PA/Zn molar ratios. Organic fertilizer substitution also increased micronutrient nutritional yields and reduced the current health burden of Fe and Zn. Under the pessimistic scenario, the OFS treatment reduced health burdens of Zn and Fe deficiencies by 2.38 and 1.31%, respectively, whereas these mitigation efficiencies substantially increased to 7.15 and 3.94% under the optimistic scenario. In conclusion, OFS improved the content and bioavailability of Fe and Zn without affecting yield, which enhanced the nutritional quality of these nutrients, and alleviate the health burden of Fe and Zn deficiency. The findings demonstrate that a 15% organic fertilizer substitution (OFS) optimally enhances wheat grain Fe and Zn bioavailability and nutritional quality while maintaining crop yield, offering region-specific evidence for sustainable agricultural practices to mitigate micronutrient deficiencies and improve human health outcomes.