AUTHOR=Tu Jiajie , Cheung Hoi-Hung , Lu Gang , Chan Clement Leung-Kwok , Chen Zijiang , Chan Wai-Yee 

TITLE=microRNA-126 Is a Tumor Suppressor of Granulosa Cell Tumor Mediated by Its Host Gene EGFL7

JOURNAL=Frontiers in Oncology

VOLUME=Volume 9 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2019.00486

DOI=10.3389/fonc.2019.00486

ISSN=2234-943X

ABSTRACT=MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in gene regulation at post-transcriptional level. In the current study, we examined the effect of miR-126 on granulosa cell tumor (GCT), a subtype of ovarian cancer. miR-126 expression was repressed in tissues from malignant GCT patients. We showed that miR-126 could inhibit cell proliferation, migration/invasion, ovarian hormone production and promote anticancer drug-induced apoptosis in vitro. The ‘tumor suppressor’ role of miR-126 was further validated in an mice GCT model in vivo. By RNA-seq, immunohistochemical staining (IHC), Western blot and luciferase reporter assay, we identified and confirmed EGFL7 as a direct functional target of miR-126 in cancer GCs. Furthermore, we found AKT signaling pathway was associated with miR-126 and EGFL7 in cancer GCs. Taken together, our results demonstrate a function of miR-126 in the repression of GCT development via regulating EGFL7.