AUTHOR=Dong Jingsi , Li Bingjie , Lin Dan , Lu Dan , Liu Chang , Lu Xingbing , Tang Xiaojun , Li Lu , Zhu Daxing , Liu Jiewei , Qiu Xiaoming , Tian Long , Zhou Qinghua 

TITLE=LincRNA00494 Suppresses Non-small Cell Lung Cancer Cell Proliferation by Regulating SRCIN1 Expression as a ceRNA

JOURNAL=Frontiers in Oncology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00079

DOI=10.3389/fonc.2020.00079

ISSN=2234-943X

ABSTRACT=<p><bold>Background:</bold> Lung cancer is the most common malignant tumor worldwide. Accumulating results have shown that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis.</p><p><bold>Patients and Methods:</bold> A total of 163 tumor tissues were collected from non-small cell lung cancer (NSCLC) patients from West China Hospital of Sichuan University. <italic>LincRNA00494</italic> is a novel lncRNA, and its expression and biological effect in NSCLC were reported in this study. NSCLC cell lines were used in this study.</p><p><bold>Results:</bold><italic>LincRNA00494</italic> is mainly distributed in the cytoplasm. <italic>LincRNA00494</italic> was downregulated in the tumor tissues compared with the adjacent non-tumor tissues. <italic>LincRNA00494</italic> expression was positively correlated with SRCIN1 expression (<italic>R</italic> = 0.57, <italic>P</italic> &lt; 0.05). Silencing of <italic>LincRNA00494</italic> in the cell lines substantially decreased SRCIN1 expression at the mRNA and protein levels, whereas overexpression of <italic>LincRNA00494</italic> enhanced the SRCIN1 levels. miR-150-3p significantly decreased the luciferase signals of <italic>LincRNA00494</italic> and SRCIN1 reporters. After transfection with miR-150-3p mimics and miR-150-3p inhibitor, overexpression of <italic>LincRNA00494</italic> decreased the proliferation of the H358 (36%) and H1299 (29%) cell lines compared with that of the control cells, as shown by CCK-8 assays, whereas silencing <italic>LincRNA00494</italic> promoted the proliferation of the H358 (47%) and H1299 (35%) cells. Tumor growth from LincRNA00494-overexpressing xenografts was significantly decreased; additionally, LincRNA00494 silencing substantially increased tumor growth compared with that of the control cells.</p><p><bold>Conclusions:</bold> Functional experiments revealed that <italic>LincRNA00494</italic> inhibited NSCLC cell proliferation, which might be related to the suppression of SRCIN1, a tumor suppressor gene, by acting as a decoy for miR-150-3p. The data showed that <italic>LincRNA00494</italic> might have antineoplastic effects during NSCLC tumorigenesis through its role as a ceRNA.</p>