REVIEW article

Front. Oncol., 06 October 2020

Sec. Cancer Metabolism

Volume 10 - 2020 | https://doi.org/10.3389/fonc.2020.555825

The Role of Long Non-coding RNAs in Cancer Metabolism: A Concise Review

  • 1. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • 2. Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran

  • 3. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Dysregulation of metabolic pathways in cancer cells is regarded as a hallmark of cancer. Identification of these abnormalities in cancer cells dates back to more than six decades, far before discovery of oncogenes and tumor suppressor genes. Based on the importance of these pathways, several researchers have aimed at modulation of these functions to intervene with the pathogenic course of cancer. Numerous genes have been shown to participate in the regulation of metabolic pathways, thus aberrant expression of these genes can be involved in the pathogenesis of cancer. The recent decade has experienced a significant attention toward the role of long non-coding RNAs (lncRNAs) in the biological functions. These transcripts regulate expression of genes at several levels, therefore influencing the activity of cancer-related pathways. Among the most affected pathways are those modulating glucose homeostasis, as well as amino acid and lipid metabolism. Moreover, critical roles of lncRNAs in regulation of mitochondrial function potentiate these transcripts as novel targets for cancer treatment. In the current review, we summarize the most recent literature regarding the role of lncRNAs in the cancer metabolism and their significance in the design of therapeutic modalities.

Introduction

Altered metabolic pathways in cancer has been attracting researchers for more than six decades when Warburg hypothesized that the tumorigenesis process is initiated by a deficient cellular respiration due to the mitochondrial function impairment (). This research area remarkably precedes the identification of the role of oncogenes and tumor suppressors in the carcinogenesis (). While normal cells obtain energy principally via mitochondrial oxidative phosphorylation (), cancer cells can fulfill the requirements of their fast and uncontrolled proliferation by excessive glycolysis and the subsequent lactic acid fermentation even in the existence of plentiful oxygen supply. This kind of aerobic glycolysis has been characterized as the Warburg effect (). Carcinogenesis process is accompanied by the extensive synchronized activation of metabolic pathways that maintain this process by dysregulation of PI3K-AKT-mTOR signaling pathways, deficiency of tumor suppressors, and induction of oncogenes (). The altered metabolic functions in the cancer cells have been shown to facilitate the attainment and preservation of malignant features. Since some of these characteristics have been detected rather commonly across many kinds of cancer cells, altered metabolic function is regarded as a hallmark of cancer (). This aberrant metabolic function facilitates anabolic growth in the course of nutrient-depleted situations, catabolism to sustain cell survival for the period of nutrient insufficiency, and protection of redox homeostasis to neutralize the metabolic influences of oncogene activation, tumor suppressor deficiency or other cellular stresses (). Such metabolic reprogramming involves several genes and molecular pathways among them are long non-coding RNAs (lncRNAs) (7). These transcripts comprise a large proportion of human transcriptome, have sizes larger than 200 nucleotides and share several features with mRNA coding genes; yet, they lack considerable open reading frames (). Not only can they regulate cell proliferation, cell death, migration, invasion and stemness properties (), but also they have critical roles in the regulation of cancer metabolism (7). The latter has been supported by a bunch of evidence which reports aberrant expression of metabolism-related lncRNAs in cancer cells. Moreover, functional studies have verified their roles in the context of cancer in some cases. The current review has focused on the role of lncRNAs in cancer metabolism and provides key examples in this regard. Based on the ever growing literature on this topic, this review cannot provide the exhaustive list of all related researches.

Mechanisms of lncRNAs Functions in Regulation of Gene Expression

LncRNAs can exert their regulatory functions through different mechanisms such as modulation of chromatin structure and DNA methylation status and interacting with transcription factors and DNA motifs, thus regulating transcription of target genes. They also influence mRNA processing to affect gene expression at post-transcriptional level. Besides, their interactions with certain proteins enable them to regulate protein translation and post-translational alterations such as phosphorylation and ubiquitination (). These transcripts can function as miRNA sponges to modulate expression of miRNA target genes or serve as precursors for miRNA or small interfering RNAs (). LncRNAs can also modulate alternative splicing processes and consequently modulate spatial and temporal expression of genes ().

Oncogenic lncRNAs That Regulate Cancer Metabolism

Oncogenic lncRNAs regulate different aspects of cancer metabolism such as glutaminolysis and lipid metabolism. For instance, UCA1 has an established role in the regulation of glutamine metabolism (). Expression of this lncRNA in bladder cancer tissues and cell lines is significantly correlated with GLS2 expression. Moreover, up-regulation of UCA1 enhances GLS2 expression and increases glutaminolysis in these cells. This function has been mediated through sponging miR-16, a miRNA that targets GLS2 (). Besides, the oncogenic lncRNA CCAT2 has been shown to alter glutamine metabolism in colon cancer (). Functional studies revealed the interaction between CCAT2 and CFIm complex, a protein complex that modulates the alternative splicing and the poly(A) site choosing in GLS transcript, leading to the privileged expression of the more aggressive variant GAC (). The lncRNA PCGEM1 has been shown to enhance glucose entry in the cells to increase aerobic glycolysis, coupling with the pentose phosphate shunt to enhance lipogenesis in prostate cancer cells ().

Several other up-regulated lncRNAs in the cancer cells have been shown to alter cancer metabolism. The metabolism-induced tumor activator 1 (MITA1) is an lncRNA which has been shown to be over-expressed in hepatocellular carcinoma (HCC) and participates in the metastatic potential of these cells. This lncRNA is remarkably activated by energy stress. This process is controlled by the LKB1-AMPK pathway and DNA methylation (). Another experiment in the HCC cells has shown correlation between expressions of the lncRNA RAET1K and both HIF1A and miR-100-5p. LncRNA RAET1K has been shown to act as a molecular sponge for miR-100-5p, thus inhibiting its expression. On the other hand, HIF1A binds with the promoter region of lncRNA RAET1K to enhance its transcription. LncRNA RAET1K knock down has inhibited proliferation and invasion of HCC cells and also overturned hypoxia-induced upsurge in lactate levels and glucose uptake. Functional studies have verified the role of the HIF1A/lncRNA RAET1K/miR-100-5p axis in regulation of hypoxia-induced glycolysis in HCC cells (). PVT1, as an up-regulated lncRNA in HCC tissues and cell lines, can directly interact with miR-150 to suppress its expression and subsequently up-regulating expression hypoxia-inducible protein 2 (HIG2) which is targeted by the miR-150. The PVT1/miR-150/HIG2 axis has been shown to regulate iron metabolism in HCC cells (15). In gastric cancer cells, LINC00152 has been shown to modulate aerobic glycolysis through modulation of miR-139-5p and PRKAA1 expressions (16). LINK-A, the upregulated lncRNA in the glioma cells, has been shown to regulate expression of lactate dehydrogenase A (LDH-A), thus enhancing glycolysis and proliferation in these cells (17). Expression of HOTAIR has been increased in hepatocellular carcinoma samples. Its expression has been enhanced under hypoxia condition. Its silencing suppressed glycolysis in these cells. Functional studies verified the role of HOTAIR as a molecular sponge for miR-130a-3p. This miRNA has been shown to inhibit expression of HIF1A. HOTAIR silencing inhibited glycolysis through modulating miR-130a-3p and HIF1A in HCC cells under hypoxic conditions (18). This lncRNA regulates cancer metabolism in pancreatic adenocarcinoma cells as well. Up-regulation of HOTAIR enhances lactate synthesis, glucose uptake and ATP synthesis. Besides, it increases HK2 expression, while HK2 up-regulation had no remarkable influence on HOTAIR expression amounts. HOTAIR has been shown to enhance cancer cell energy metabolism in this kind of cancer through increasing HK2 expression (19). UCA1 has been shown to increase mitochondrial function in bladder cancer cells. This lncRNA acts as a molecular sponge for miR-195 to control mitochondrial function through enhancing expression of ARL2. The role of UCA1 through miR-195/ARL2 axis in promotion of bladder tumor growth has been verified in animal models (20). Figure 1 shows a summary of role of UCA1 in the regulation of cancer metabolism.

Figure 1

Table 1 summarizes the role of oncogenic lncRNAs in the cancer metabolism in all kinds of human malignancies.

Table 1

Type of cancerlncRNANumbers of clinical samplesAssessed cell lineTargets/ regulatorsSignaling pathwaysFunctionPatient's prognosisReferences
Hepatocellular carcinoma (HCC)MITA113 pairs of liver tumors and adjacent normal liversHepG2, A549, U87, PC3, Huh7, HCCLM3, SK-Hep1, SMMC-7721, LO2, HGC27, U251LKB1, AMPKSlugEnergy stress through the LKB1-AMPK pathway could induce MITA1 expression. MITA1 could been induced by glucose starvation in a time-dependent manner.–()
RAET1K66 pairs of HCC and adjacent normal tissuesL02, HCCLM3, HepG2, huh7, Hep3BmiR-100-5p, LDHA–The HIF1A/lncRNA-RAET1K/miR-100-5p/LDHA axis could modulateglycolysis under hypoxia in HCC cells and affect HCC progression–()
LINC0163874 pairs of HCC and adjacent normal tissuesSNU-398, SNU-182GLUT1–lncRNA-LINC01638 by increasing glucose uptake via targeting GLUT1 could promote cancer cell proliferation in HCC–(23)
PVT147 pairs of liver tumors and adjacent normal liversL-02, SK-HEP-1, Hep G2, SMMC-7721, BEL-7402, Hep3B2.1-7, QGY-7703X4miR-150, HIG2–PVT1 via by regulating miR-150/HIG2 axis could modulate IRE/IRP regulatory system and cellular iron uptake/metabolism–(15)
HOTAIR38 pairs of hepatocellular carcinoma and adjacent normal tissuesHepG2, Huh7, LO2, 293TmiR-130a-3p, HIF1A, HK2Knockdown of HOTAIR possibly via targeting miR-130a-3p/HIF1A could inhibit glycolysis in hepatocellular carcinoma cells stimulated with hypoxia–(18)
HOTTIP104 pairs of HCC and adjacent normal tissuesSMMC7721, HepG2, Hep3BmiR-192, miR-204, GLS1–miR-192 and miR-204 could suppress HOTTIP expression. miR-192/-204-lncRNA-HOTTIP axis via inhibiting GLS1 could interrupt HCC glutaminolysis.Poor prognosis(24)
Ftx73 pairs of HCC and adjacent normal tissuesLO2, Huh7, SMMC-7721, Bel-7402TNF-α, leptin, PDK1, GLUT1, GLUT4PPARγLncRNA-Ftx via targeting GLUTs through the PPARγ pathway could promote aerobic glycolysis in hepatocellular carcinoma.Poor prognosis(25)
Glioblastoma (GBM)XISTGSE50161 and GSE44971 microarraysU87MG, U251, U343, Hs683, LN215, A172, HA1800miR-126IRS1/PI3K/AktOverexpression of lncRNA-XIST via miR-126/IRS1/PI3K/Akt pathway could enhance glucose metabolism in glioma. Knockdown of lncRNA-XIST could reduce GLUT1 and GLUT3 levels.–(26)
UCA142 pairs of glioma tissues as well as the peritumoral brain edema (PTBE) tissuesU251, U87MGmiR-182, PFKFB2, CXCL14–LncRNA-UCA1/miR-182 axis by interacting PFKFB2 could induce a glycolytic phenotype in glioma.–(27)
LINK-A–U87, U251, HasLDHA–LncRNA-LINK-A via regulating LDHA could promote glycolysis and proliferation in GBM cells.–(17)
LINC0068956 pairs of glioma and adjacent normal tissues—GSE datasetU87, U251, NHA, 293TmiR-338-3p, PKM2–LncRNA-LINC00689 via targeting miR-338-3p/PKM2 axis could promote glycolysis in glioma cells.Poor prognosis(28)
Breast cancerYIYA (LINC00538)35 pairs of breast cancer and adjacent normal tissuesMDA-MB-231, MCF7, BT474, 293TPFKFB3, CDK6, FBXW7–LncRNA-YIYA could promote glycolysis in breast cancer.Poor prognosis(29)
HISLABreast cancer samples (n = 453)MDA-MB-231, MDA-MB-468, BT-474, MCF-7GLUT1, GLUT3, HK2–Tumor-associated macrophages (TAMs) could enhance the aerobic glycolysis of breast cancer cells by extracellular vesicle (EV)-packaged lncRNA-HISLA. Blocking EV-transmitted lncRNA-HISLA via targeting GLUT1, GLUT3, and HK2 could inhibit the glycolysis in breast cancer cells.Poor prognosis()
BCAR4Breast cancer patients (n = 123)MDA-MB-231, MDA-MB-468, 293THK2, PFKFB3Hippo, HedgehogBCAR4/GLI2 by upregulating glycolytic enzymes HK2 and PFKFB3 could promote glycolysis in breast cancer cells. Overexpression of BCAR4 could increase glucose uptake and lactate production.Poor prognosis(30)
SNHG730 pairs of breast cancer and adjacent normal tissuesMCF10A, MDA-MMB-436, HS578T, SKBR3, MDA-MB-231, MCF-7miR-34a-5p, LDHA, c-Myc–c-Myc through the lncRNA-SNHG7/miR34a-5p/LDHA axis could regulate glycolysis in breast cancer cells.–(31)
FGF13-AS130 pairs of breast cancer and adjacent normal tissuesMCF-10A, MCF-7, T47D, MDA-MB-453, MDA-MB-468, MDA-MB-231, 293TGF13-AS1, IGF2BPs, c-Myc–Overexpression of lncRNA-FGF13-AS1 via FGF13-AS1/IGF2BPs/Myc feedback loop could inhibit glycolysis in breast cancer cells.Poor prognosis(32)
Bladder cancer (BC)UCA1Normal bladder tissue (n = 6), BC tissues (n = 22), and adjacent tissues (n = 10)5637, UMUC2miR-195, ARL2–Overexpression of lncRNA-UCA1 via downregulating miR-195 and upregulating ARL2 expression could promote mitochondrial function and ATP production of BC.–(20)
UCA1–UMUC-2, 5637HK2, miR-143mTOR/STAT3LncRNA-UCA1 via upregulating HK2 through the mTOR/STAT/miR-143 pathway could promote glycolysis in BC cells.–(21)
UCA1Normal bladder tissues (n = 6), adjacent cancer tissues (n = 10), bladder cancer tissues (n = 35)UMUC2, 5637, BLS-211, BLZ-211miR-16, GLS2–LncRNA-UCA1 via miR-16/GLS2 axis could promote glutamine metabolism in bladder cancer.–()
Osteosarcoma (OS)TUG1–hFOB1.19, Saos-2, U2OS, HOS, MG63HK2–Knockdown of lncRNA-TUG1 via targeting HK2 could inhibit glucose consumption and lactate production in osteosarcoma cells.–(33)
HAND2-AS1–MG-63, SAOS-2, U-2OS, HOS, SW1353FBP1, HIF1αAKTKnockdown of lncRNA-HAND2-AS1 via interacting with FBP1 and promoting HIF-1α could promote glucose metabolism under energy stress condition.–(34)
PVT146 pairs of OS and adjacent normal tissuesU2OS, Saos-2, 143B, MG-63, hFOBmiR-497, HK2–LncRNA-PVT1 via regulating miR-497/HK2 axis could promote glycolysis and tumor progression in OS.Poor prognosis(35)
Endometrial carcinoma (ECa)SNHG16GEPIA databaseHEC-1B, HEC-1A, RL95-2, AN3CA, EMCmiR-490-3p, HK2–TFAP2A/lncRNA-SNHG16 via targeting miR-490-3p/HK2 axis could regulate glycolysis of ECa cells.Poor prognosis(36)
Colon cancer (CC)AWPPHCC patients (29), normal controls (n = 42)FHC, HT-29GLUT-1–Although the glucose uptake was not directly measured, knockdown of lncRNA-AWPPH via downregulating GLUT-1 could inhibit colon cancer cell proliferation.Poor prognosis(37)
Lung cancerLINC0085735 pairs of lung cancer and adjacent normal tissuesH1229, H838, BEAS-2BmiR-1179, SPAG5–LncRNA-LINC00857 by targeting miR-1179/SPAG5 axis could regulate glycolysis in lung adenocarcinoma.–(38)
IGFBP4-1159 pairs of lung cancer and adjacent normal tissuesA549, PC-9, GLC-82, 16HBE, HBE-PIC, BEP-2D, BEAS-2B, 293T, L78HK2, PDK1, LDHA–Overexpression of lncRNA-IGFBP4–1 via targeting HK2/PDK1/LDHA could affect energy metabolism and promote lung cancer progression.–(39)
Non-small cell lung cancer (NSCLC)UCA1–16-HBE, A549, H1299, H522, 95D, H358PKM2mTORKnockdown of UCA1 by suppressing PKM2 through inactivation of the mTOR pathway could inhibit the glycolysis of NSCLC cells. UCA1 silencing could reduce the glucose consumption and lactate production.–(40)
CRYBG323 clinical lung cancer tissues and 4 normal lung tissuesA549, H1299, Beas-2BLDHA–lncRNA-CRYBG3 by interacting with LDHA could regulate glycolysis in lung cancer cells–(41)
NORAD80 pairs of NSCLC and adjacent normal tissuesA549, H1975, H1650, LK-2, H1299, H460, HBEmiR-136-5p, E2F1–LncRNA-NORAD via targeting miR-136-5p/E2F1 axis could promote glycolysis in NSCLC.–(42)
BCYRN120 pairs of NSCLC and adjacent normal tissuesA549, H460, H1299, 16HBEmiR-149, PKM2–LncRNA-BCYRN1 by regulating the miR-149/PKM2 axis could promote glycolysis in NSCLC. BCYRN1/miR-149/PKM2 signaling pathway is involved in Warburg effect.–(43)
LINC0112392 pairs of NSCLC and adjacent normal tissuesA549, H1299, H1650, H1975, PC9, HBEc-Myc, miR-199a-5p–LncRNA-LINC01123 via miR-199a-5p/c-Myc axis could promote aerobic glycolysis in NSCLC.Poor prognosis(44)
Prostate cancer (PC)PCA320 pairs of PC and adjacent normal tissuesRWPE-1, C4-2, 22Rv1, LNCaP, PC3CDK4, miR-1–LncRNA-PCA3 via targeting miR-1/CDK4 axis could regulate glycolysis in PC.–(45)
SNHG16Prostate carcinoma (n = 52) and normal prostate (n = 36) tissues22Rv1, HPrECGLUT-1–Inhibition of lncRNA-SNHG16 by downregulating GLUT1 expression could reduce glucose uptake in prostate carcinoma–(46)
PCGEM1–LNCaP, PC3, 293T, LNCaP/PCGEM1, LNCaP/shPCGEM1c-Myc–lncRNA-PCGEM1 via targeting c-Myc could promote glucose uptake for aerobic glycolysis; therefore, it could regulate the metabolism of tumor–(47)
Colorectal cancer (CRC)SNHG16314 colorectal adenocarcinomas and 292 adjacent normal colon mucosa samplesColo205, DLD1, HCT116, HCT15, HT29, LS174T, SW480, SW620, CaCo2ASCL2, ETS2, c-MycWntLncRNA-SNHG16 via Wnt pathway could affect some genes, such as HSD17B7 and INPP5D, involved in lipid metabolism in colorectal cancer–(48)
LINRIS118 pairs of CRC and adjacent normal tissuesCCD841, LOVO, RKO, CW2, SW1116, SW480, DLD-1, HCT116, HT29, COLO205IGF2BP2–LncRNA-LINRIS via stabilizing IGF2BP2 could promote aerobic glycolysis in CLCPoor prognosis(49)
GLCC195 pairs of CRC and adjacent normal tissuesSW1116, LoVo, SW480, Caco2, HT29, RKO, DLD-1, HCT116c-Myc, LDHA, HSP90LncRNA-GLCC1 via stabilizing c-Myc could promote glucose metabolism in CRCPoor prognosis(50)
MAFG-AS152 pairs of colorectal cancer and adjacent normal tissuesHCT-116, HT29, SW480, LoVomiR-147b, NDUFA4, PDK1, PFK1, PKM2–LncRNA-MAFG-AS1 by sponging miR-147b and activating NDUFA4 could promote glycolysis in colorectal cancer–(51)
LINC00265GSE21510 datasetSW480, Caco-2, SW620, HCT116, HT29, HIECmiR-216b-5p, TRIM44–LncRNA-LINC00265 via regulating miR-216b-5p/TRIM44 axis could promote glycolysis and lactate production in CRCPoor prognosis(52)
Cervical cancer (CC)UCA1–HeLa, SiHa, HeLa-IRR, SiHa-IRR (radiation dosage: 76 Gy)HK2, PKM, GLUT-1–In SiHa-IRR and HeLa-IRR cells, the expression of lncRNA-UCA1 and the activity of glycolysis are increased. LncRNA-UCA1 via the HK2/glycolytic pathway could regulate radioresistance in cervical cancer.–(53)
UCA120 pairs of cervical cancer and adjacent normal tissuesHEC251, HEC-1B, Hela, N3CA, HEC-1A, RL95-2, Ishikawa3h12miR-493-5p, HK2–LcRNA-UCA1 via targeting miR-493-5p/HK2 axis could modulate the glycolysis in cervical cancer–(54)
LNMICC211 paraffin-embedded tissues of cervical Cancer, 92 pairs of CC and adjacent normal tissuesSiHa, CaSki, ME180, MS751, HeLa, HeLa229, HLECsmiR-190, FASN, ACC1, ACOX1, CPT1A, FABP5–LncRNA-LNMICC could promote lymph nodes (LN) metastasis in cervical cancer via affecting fatty acid metabolism by recruiting the NPM1 to the FABP5 promoter and targeting miR190Poor prognosis(41)
Epithelial ovarian cancer (EOC)LINC0009248 pairs of serous ovarian cancer and adjacent normal tissuesSKOV-3, A2780PFKFB2–The expression of lncRNA-LINC00092 is increased in A2780s ovarian cancer cell treated with recombinant CXCL14 protein. LINC00092 via targeting PFKFB2 could act in cancer-associated fibroblasts (CAF) to drive glycolysis in ovarian cancer.Poor prognosis(55)
SNHG318 pairs of EOC and adjacent normal tissues—TCGA DataIOSE80, SKOV3, TOV-21G, OVCAR-3EIF4AIII, PKM, PDHB, IDH2, UQCRH, Kreb's cycle, OXPHOS–LncRNA-SNHG3 via targeting several pathways could regulate energy metabolism of EOCPoor prognosis(56)
NRCPSerous ovarian cancer (n = 29), normal ovarian (n = 11)SKOV3, A2780STAT1–The silencing of lncRNA-NRCP could reduce the levels of glucose-6-phosphate isomerase ALDOA and ALDOC. lncRNA-NRCP via STAT1 could promote glycolysis in ovarian cancer cells.–(57)
Pancreatic cancerXLOC_00639021 pairs of pancreatic tumors and adjacent normal tissuesCFPAC-1, BxPC-3c-Myc, GDH1–LncRNA-XLOC_006390 via targeting GDH1 could promote glutamate metabolism by stabilizing c-Myc in pancreatic cancer.–(58)
Pancreatic ductal adenocarcinoma (PDAC)PVT130 pairs of PDAC and adjacent normal tissuesHPAC, DANG, BXPC3, PANC1, ASPC-1, H6C7miR-519d-3p, HIF-1A–Upregulation of lncRNA-PVT1 via regulating the miR-519d-3p/HIF-1A axis could promote glycolysis in PDAC.Poor prognosis(59)
HOTAIRPancreatic adenocarcinoma (n = 78), adjacent healthy tissues (n = 51)BxPC-3, Capan-2HK2–Overexpression of lncRNA-HOTAIR via targeting HK2 could increase glucose uptake, lactate production, and ATP production in pancreatic adenocarcinomaPoor prognosis(19)
Esophageal squamous cell carcinoma (ESCC)LOC148709––PFKFB3–LncRNA-LOC148709 by binding to and stabilizing PFKFB3 could play an important role in glycolytic reprogramming in ESCCPoor prognosis(60)
HepatoblastomaHR1–Huh7, 293T, HepG2, Hep2, HeLa, MCF7, K562, RD, THP-1, TZMBL, PANC-1SREBP-1c–LncRNA-HR1 by inhibiting SREBP-1c could regulate hepatic lipid metabolism–(61)
AT102202–HepG2HMGCR–The expression of lncRNA-AT102202 is upregulated in HepG2 cells treated with epigallocatechin-3-gallate (EGCG). AT102202 via targeting HMGCR could play an important role in cholesterol metabolism.–(62)
Gastric cancer (GC)MACC1-AS1TCGA database, 123 formalin-fixed and paraffin-embedded (FFPE) GC tissue samplesAGS, GES-1, BGC803, BGC823, MKN45, SGC7901MACC1, GLUT1, HK2, G6PD, MCT1AMPK/Lin28LncRNA-MACC1-AS1 via AMPK/Lin28 signaling-mediated mRNA stability of MACC1 could promote metabolic plasticity in GC cells.Poor prognosis(63)
LINC00152Pairs of GC and adjacent normal tissuesSUN16, AGS, MKN28, SGC7901, BGC823miR-139-5p, PRKAA1–LncRNA-LINC00152/miR-139-5p by regulating PRKAA1 could facilitate aerobic glycolysis in GC cells.–(16)
RP11-605F14.2, TBC1D3P5, BC130595, LINC00475, RP11-19P22.6, BC080653, XLOC-004923, AFAP1-AS1, EPB49, RP11-296I10.3104 pairs of GC and adjacent normal tissues–––Metabolic pathway-associated lncRNAs have a crucial role in the pathogenesis of GC.–(64)
MelanomaH1930 pairs of malignant melanoma and adjacent normal tissuesA375, SK-MEL-1, SK-MEL-5miR-106a-5p, E2F3–LncRNA-H19 via miR-106a-5p/E2F3 axis could promote glucose metabolism in malignant melanoma.Poor prognosis(65)
Multiple myeloma (MM)PDIA3PPlasma cells derived from bone marrow of MM patients (n = 24) and normal healthy donors (n = 52)OPM-2, U266, RPMI-8226, NCI-H929, MM.1Sc-MycG6PD/PPPLncRNA-PDIA3P by interacting with c-Myc through G6PD/PPP pathway could regulate cell proliferation multiple myeloma.Poor prognosis(66)
Nasopharyngeal carcinoma (NPC)ANRIL88 pairs of NPC and adjacent normal tissuesNP69, N5-Tert, CNE2, CNE1, SUNE1, HONE1, HK1, S26, S18, 5-8F, 6-10B, HNE1LDHA, GLUT1mTORLncRNA-ANRIL via LDHA/GLUT1/mTOR pathway could promote cell glucose metabolism in NPC cells.–(67)
XIST25 pairs of NPC and adjacent normal samplesHK-1, C666-1, NP69miR-381-3p, NEK5–Knockdown of XIST via downregulating NEK5 and upregulating miR-381-3p expression could inhibit hypoxia-induced glycolysis and metastasis in NPC cells.–(68)
Intrahepatic cholangiocarcinoma (ICC)TUG1102 pairs of ICC and adjacent normal tissuesHuH28, HuCCT1, RBE, HCCC- 9810, HIBEpiCmiR-145, Sirt3, GDH–LncRNA-TUG1 via miR-145/Sirt3/GDH axis could regulate glutamine metabolism and promote cancer progression.Poor prognosis(69)
Oral squamous cell carcinoma (OSCC)ELF3-AS1112 pairs of OSCC and adjacent normal tissuesSCC090,SCC25GLUT1–LncRNA-ELF3-AS1 via positively regulating GLUT1 expression could promote glucose uptake in OSCC cells.–(70)
P231544 pairs of OSCC and adjacent normal tissues–miR-378a-3p, GLUT1–LncRNA-P23154 by regulating GLUT1-mediated glycolysis could promote the invasion-metastasis potential of OSCC–(71)
Acute myeloid leukemia (AML)UCA1Bone marrow samples of 27 pediatric AML patientsHL60, HS-5, HL60/ADRHK2, miR-125a–The expression of lncRNA-UCA1 is upregulated following ADR-based chemotherapy.UCA1 Knockdown by inhibiting glycolysis through the miR-125a/HK2 pathway could suppress the chemoresistance in pediatric AML cells.–(72)
ANRILAML patients (n = 109), normal controls (n = 14)MOLM-13, HL-60LDHA, GLUT1AdipoR1, AMPK, SIRT1LncRNA-ANRIL via targeting LDHA/GLUT1 and through modulating the glucose metabolism pathway of AdipoR1/AMPK/SIRT1 could regulate AML development.–(73)
Head and neck squamous cell carcinoma (HNSCC)HNGA14 pairs of HNSCC and adjacent normal tissues–miR-375, SCL2A1–LncRNA-HNGA1 via regulating miR-375/SCL2A1 could promote aerobic glycolysis in HNSCC.–(74)
–NBR2–MDA-MB-23, 293T, 786-O, SLR20, BT549GLUT1, AMPK, mTORC1–Treatment with phenformin has increased the expression of lncRNA-NBR2. Therefore, in response to phenformin treatment, lncRNA-NBR2 could regulate GLUT1 expression and glucose uptake in cancer cells.–(75)

The role of oncogenic lncRNAs in the cancer metabolism.

Tumor Suppressor lncRNAs That Regulate Cancer Metabolism

A number of studies have assessed the association between tumor suppressor lncRNAs and metabolic pathways. NEF as a down-regulated lncRNA in NSCLC tissues has been shown to regulate cell proliferation and glucose uptake in these cells through modulation of GLUT1 expression. Thus, this lncRNA can target glucose transportation to suppress lung tumorigenesis (76). LINC01537 as another tumor suppressor lncRNA has been demonstrated to enhance cellular sensitivity to nilotinib. This lncRNA also targets phosphodiesterase 2A (PDE2A) and enhance it expression through RNA–RNA interaction. Based on the role of PDE2A in energy metabolism, Warburg effect and mitochondrial respiration, LINC01537 has been identified as a regulator of cancer metabolism (77). The lncRNA GASL1 has been shown to enhance Bcl-2 expression, while down-regulating GLUT-1 expression. Thus, the role of this lncRNA in suppression of proliferation of prostate cancer cells has been exerted through modulation of metabolism (78). LINC01554, the down-regulated lncRNA in hepatocellular carcinoma has been shown to be suppressed by miR-365a. This lncRNA enhances the ubiquitin-mediated destruction of PKM2 and suppresses Akt/mTOR signaling pathway to stop aerobic glycolysis in hepatocellular cancer cells (79). FILNC1 has been identified as an energy stress-induced lncRNA. FILNC1 silencing in renal cancer cells lessens energy stress-induced apoptosis and considerably induces progression of this type of cancer. Notably, FILNC1 silencing increases glucose uptake and lactate synthesis via induction of c-Myc. In energy stress conditions, this lncRNA binds with AUF1, a c-Myc interacting protein. Thus, it prevents AUF1 from binding with c-Myc transcript, resulting in under-expression of c-Myc protein (80). Expression of the lncRNA HAND2-AS1 has been decreased in osteosarcoma tissues and serum samples of the affected patients compared with control samples. There was a significant association between serum levels of this lncRNA and tumor size. Notably, in vitro studies revealed that HAND2-AS1-silencing enhances osteosarcoma cell proliferation, upsurges glucose uptake and increases GLUT1 levels. Thus, HAND2-AS1 has a tumor suppressor role in osteosarcoma through modulating glucose metabolism (81). GATA6-AS is another tumor suppressor lncRNA that regulates expression of GLUT1. Up-regulation of this lncRNA has reduced glucose uptake and GLUT1 expression in the mantle cell lymphoma. Thus, the lncRNA GATA6-AS might suppress cancer cell proliferation through decreasing GLUT1 expression (82). The lncRNA MORT has a similar role in suppression of glucose uptake and GLUT1 expression in prostate cancer cell lines (83). In prostate cancer cells, up-regulation of GASL1 has enhanced Bcl-2 expression and decreased GLUT-1 levels (78). CASC8 is also involved in the regulation of the glycolysis in bladder cancer cells through modulating expression of the fibroblast growth factor receptor 1 (FGFR1). The interaction between this lncRNA and FGFR1 has been shown to suppress FGFR1-associated lactate dehydrogenase A phosphorylation, which decreases the lactate synthesis from pyruvate (84). Table 2 summarizes the role of tumor suppressor lncRNAs in the cancer metabolism.

Table 2

Type of cancerlncRNANumbers of clinical samplesAssessed cell lineTargets/ regulatorsSignaling pathwaysFunctionPatient's prognosisReferences
Non-small cell lung cancer (NSCLC)NEF33 pairs of NSCLC and adjacent normal tissuesNCI-H23, NCI-H522, NCI-H520, NCI-H2170GLUT1–Overexpression of lncRNA-NEF via downregulating GLUT1 expression could inhibit glucose uptake in NSCLC cellsPoor prognosis(76)
Lung cancerLINC01537243 pairs of cancerous and corresponding non-cancer lung tissuesA549, PC-9, 293TPDE2A, GLUT1–Overexpression of lncRNA-LINC01537 via targeting PDE2A could attenuate the Warburg effect and mitochondrial respiration. Therefore, it is involved in energy metabolism–(77)
Hepatocellular carcinoma (HCC)LINC01554167 pairs of HCC and adjacent normal tissuesMIHA, BEL7402, QGY7701, QGY7703, SMMC7721, PLC8024, HepG2, Huh7, Hep3BmiR-365a, PKM2Akt/mTORLncRNA-LINC01554-mediated glucose metabolism reprogramming via downregulating PKM2 expression and inhibiting Akt/mTOR signaling pathway could suppress tumorigenicity in HCC.Poor prognosis(79)
Renal cancerFILNC123 pairs of ccRCC and normal kidney samples293T, RCC4, 786-O, 769P, SLR20, UMRC2AUF1–LncRNA-FILNC1 deficiency via targeting AUF1 could increase glucose uptake and lactate production in renal tumor.Poor prognosis(80)
OsteosarcomaHAND2-AS1Osteosarcoma patients (n = 48), normal controls (n = 44)MG-63, SAOS-2, hFOB-2GLUT1–Knockdown of lncRNA-HAND2-AS1 via upregulating GLUT1 expression could promote glucose uptake in osteosarcoma.–(81)
Mantle cell lymphoma (MCL)GATA6-ASPlasma samples of patients with MCL (n = 47) and healthy controls (n = 42)JVM-2, Z-138GLUT1–Overexpression of lncRNA-GATA6-AS by downregulating GLUT1 expression could inhibit glucose uptake in mantle cell lymphoma.–(82)
Bladder cancerCASC850 pairs of bladder cancer and adjacent normal tissuesSW780, J82, UMUC3, T24, 5637FGFR1–Overexpression of CASC8 through interacting with FGFR1 and inhibiting FGFR1-mediated LDHA phosphorylation could suppress glycolysis in bladder cancer cell.–(84)
Prostate carcinoma (PC)GASL166 pairs of PC and adjacent normal tissuesHprEC, 22Rv1, DU145GLUT-1, Bcl-2, Bax–GASL1 via targeting GLUT-1, which has a major role in glucose metabolism, could promote the expression of apoptosis-associated proteins in PC cells; hence, could inhibit the growth.Poor prognosis(78)
MORT60 pairs of PC and adjacent normal tissues22Rv1GLUT-1–lncRNA-MORT by inhibiting glucose uptake via inactivating GLUT-1 expression could suppress tumor cell proliferation in PC.–(83)
Gastric cancerTUG1, RP11-555H23.1, RP1-257I20.13, UGP2, GCSHP3, XLOC-000889104 pairs of gastric carcinoma and adjacent normal tissues–––Metabolic pathway-associated lncRNAs have a crucial role in gastric cancer.–(64)
TOPORS-AS1103 pairs of GC and adjacent normal tissues–NDUFB6–The metabolism-associated lncRNAs have important roles on metabolism of cancers. TOPORS-AS1 via targeting NDUFB6 may affect glucose metabolism in gastric cancer cells.-(85)
Several human cancersNBR2–MDA-MB-23, 293T, HeLa, A549, 786-O, DU145, MCF-7, BT-549, SLR20LKB1AMPKNBR2 via LKB1–AMPK pathway could engage a metabolic checkpoint under energy stress.Poor prognosis(86)
EPB41L4A-AS1TCGA and GEO datasetsHeLa, HepG2HDAC2, HIF-1α, VDAC1, VHL–lncRNA-EPB41L4A-AS1 via mediating nucleolar translocation of HDAC2 could regulate glycolysis and glutaminolysis in cancer.–(87)
Colorectal cancerMEG380 colorectal cancer tissue samples and adjacent normal mucosal samplesDLD-1, RKOc-Myc–Vitamin D-activated lncRNA-MEG3 via degrading c-Myc could suppress aerobic glycolysis of colorectal cancer cells.Poor prognosis(88)

The role of tumor suppressor lncRNAs in the cancer metabolism.

Significance of Metabolism-Related lncRNAs in Cancer Diagnosis and Prognosis

Consistent with the crucial roles of metabolism-related lncRNAs in the evolution of human cancers, dysregulation of these lncRNAs have been associated with patients' outcome. Moreover, transcript levels of them have been exploited as diagnostic markers in diverse cancers. For instance, serum levels of the lncRNA AWPPH have been elevated in patients with colon cancer compared with normal subjects. Receiver operating characteristic (ROC) curve analysis has shown the appropriateness of serum levels of this lncRNA for diagnosis of colon cancer with diagnostic power of 0.84 (37). Serum levels of the lncRNA NEF have been shown to have diagnostic power of 0.94 for NSCLC. Moreover, the overall survival rate of patients with elevated serum levels of this lncRNA was remarkably better compared with those having low level of this lncRNA. Taken together, serum concentrations of NEF might be considered as diagnostic and prognostic markers for this kind of cancer (76). In patients with cholangiocarcinoma, Kaplan-Meier survival analysis has demonstrated decreased overall survival (OS) and disease-free survival (DFS) in patients with high levels of TUG1 expression. Univariate analysis has also verified the effect of TUG1 expression levels in determination of OS and DFS (69). Several other lncRNAs that regulate cancer metabolism have been identified as diagnostic/prognostic markers in cancer. Table 3 summarizes the results of studies which reported diagnostic/prognostic significance of these lncRNAs.

Table 3

Sample numberAUCSensitivitySpecificityKaplan-Meier analysisUnivariate cox regressionMultivariate cox regressionReferences
Colon cancer patients (29), normal controls (n = 42)0.84 for AWPPH–––––(37)
33 pairs of NSCLC and adjacent normal tissues0.94––Patients with a high-level lncRNA-NEF had a higher rate of OS.––(76)
Osteosarcoma patients (n = 48), normal controls (n = 44)0.86––––There was a significant correlation between tumor size and serum levels of HAND2-AS1(81)
Breast cancer samples (n = 453)–––Patients with a high level lncRNA-HISLA had lower rate of OS.––()
80 colorectal cancer tissue samples and adjacent normal mucosal samples–––Patients with a low-level lncRNA-MEG3 had a lower rate of OS.––(88)
30 pairs of pancreatic cancer and adjacent normal tissues–––Patients with a high-level lncRNA-PVT1 had a lower rate of OS.––(59)
104 pairs of gastric carcinoma and adjacent normal tissues0.65 for lncRNA- RP11-555H23.181% for lncRNA- RP11-555H23.162% for lncRNA- RP11-555H23.1––RP11-555H23.1 expression was significantly correlated with TNM stage(64)
104 pairs of HCC and adjacent normal tissues–––Patients with a high-level lncRNA-HOTTIP had a lower rate of OS.––(24)
18 pairs of ovarian cancer and adjacent normal tissues—TCGA Data–––Patients with a high-level lncRNA-SNHG3 had a lower rate of OS.––(56)
56 pairs of glioma and adjacent normal tissues—GSE dataset–––Patients with a high-level lncRNA-LINC00689 had a lower rate of OS.––(28)
30 pairs of malignant melanoma and adjacent normal tissues–––Patients with a high-level lncRNA-H19 had a lower rate of OS.––(65)
Pancreatic adenocarcinoma (n = 78), adjacent healthy tissues (n = 51)–––Patients with a high level lncRNA-HOTAIR had lower rate of OS.––(19)
66 pairs of prostate cancer and adjacent normal tissues0.9076 for tissue, 0.8811 for serum––Patients with a low-level lncRNA-GASL1 had a lower rate of OS.–Expression levels of GASL1 were significantly associated with tumor size.(78)
88 pairs of NPC and adjacent normal tissues–––––ANRIL expression could serve as an independent predictor of disease-free survival and overall survival(67)
102 pairs of ICC and adjacent normal tissues––––No association was observed between TUG1 expression and age, sex, and tumor sizeTUG1 expression was associated with tumor stage, intrahepatic metastasis, lymph node metastasis, and perineural invasion(69)
Serous ovarian cancer (n = 29), normal ovarian (n = 11)–––Patients with a high-level lncRNA-NRCP had lower rate of OS.––(57)
118 pairs of CRC and adjacent normal tissues–––Patients with a high-level lncRNA-LINRIS had a lower rate of OS.––(49)
92 pairs of NSCLC and adjacent normal tissues–Patients with a high-level lncRNA-LINC01123 had a lower rate of OS.––(44)
211 paraffin-embedded tissues of the cervical Cancer, 92 pairs of CC and adjacent normal tissues–––Patients with a high-level lncRNA-LNMICC had lower rates of OS and DFS.–A higher LNMICC expression was correlated with tumor size, lymph node metastasis, lymphovascular space invasion, stromal invasion, recurrence, and vital status(41)
23 pairs of ccRCC and normal kidney samples–––Patients with a high-level lncRNA-FILNC1 had a lower rate of OS.––(80)
95 pairs of CRC and adjacent normal tissues––––lncRNA-GLCC1 expression was an independent predictor of CRC aggressivenessthe lncRNA-GLCC1 expression is associated with tumor size(50)
46 pairs of OS and adjacent normal tissues–––Patients with a high-level lncRNA-PVT1 had a lower rate of OS.––(35)
GSE21510 dataset–––Patients with a high level lncRNA-LINC00265 had lower rate of OS.––(52)
167 pairs of HCC and adjacent normal tissues–––Patients with a low-level lncRNA-LINC01554 had a lower rate of OS.–LINC01554 was associated with tumor invasion, tumor size, tumor staging in HCC patients.(79)
TCGA database, 123 formalin-fixed and paraffin-embedded (FFPE) GC tissue samples–––Patients with a high-level lncRNA-MACC1-AS1 had lower rates of OS and DFS.–MACC1-AS1 and TNM stage were independent prognostic factors in GC patients.(63)
48 pairs of serous ovarian cancer and adjacent normal tissues–––Patients with a high-level lncRNA-LINC00092 had lower rates of OS and PFS.––(55)
Plasma cells derived from bone marrow of MM patients (n = 24) and normal healthy donors (n = 52)–––Patients with a high-level lncRNA-PDIA3P had lower rate of OS.––(66)

The role of metabolism-related lncRNAs in cancer diagnosis and prognosis (DFS, disease free survival; OS, overall survival; PFS, progression free survival).

Discussion

The carcinogenesis process is associated with high glucose uptake, lactate over-production, aerobic glycolysis as well as glutamine and lipid metabolism (89). The above-mentioned data support the role of lncRNAs in these metabolic pathways in the context of cancer. Notably, HCC has been the most investigated cancer type regarding the role of lncRNAs in the metabolic pathways. Apart from the function of lncRNAs in this regard, metabolic changes have been previously recognized to evidently distinguish HCC tumors. Several clinical parameters that are presently utilized to evaluate liver functions reveal alterations in both enzyme activity and metabolites. Actually, alterations in glucose and acetate consumption are regarded as effective clinical means for classification of patients with HCC. Besides, elevated serum lactate can differentiate HCC from healthy individuals, and serum lactate dehydrogenase is applied as a determinant of prognosis of HCC patients under therapeutic regimens (90). Thus, it is not surprising that the role of lncRNAs has been vastly assessed in this context. The underlying mechanism of participation of lncRNAs in the regulation of metabolic pathways has been clarified in several cases. Glucose transporters (GLUTs) as important modulators of glucose utilization which are commonly dysregulated in cancer (91), have been shown to be targeted by several lncRNAs such as LINC01638, Ftx, XIST, YIYA (LINC00538), HISLA, AWPPH, and UCA1. Most notably, several lncRNA/ miRNA/mRNA comprising axes have been shown to modulate cancer metabolism. Therefore, the complex interactions between these trios should be considered in the design of any therapeutic option. Moreover, numerous lncRNAs have direct or indirect interactions with the well-known oncogene c-Myc. This oncogene is an important modulator of pathways that regulate metabolism of glucose, glutamine and lipid in cancer (92). Thus, all of these lncRNAs are putative regulators of different aspects of cancer metabolism.

Several oncogenic lncRNAs mainly exert their effects through modulation of these pathways. Thus, modulation of expression of these lncRNAs through application of antisense oligonucleotides or CRISPR/Cas9-based modalities can be regarded as a therapeutic option in cancer. Yet, the main obstacles in this regard are their off-target effects or unstable efficiency resulting from the space-time related features of lncRNAs (93). Small interfering (si)RNA-mediated silencing of oncogenic lncRNAs has been hampered by unavailability of efficient delivering systems. Yet, this such hurdle has been rather solved by the advent of biocompatible nanoparticle delivery systems (86).

The relevance of metabolism-associated lncRNAs in the treatment of cancer has been highlighted by a number of studies. For instance, the lncRNA-UCA1 has been shown to modulate radioresistance in cervical cancer cell through the HK2/glycolytic pathway (53). The same lncRNA has been shown to be upregulated in AML patients after Adriamycin (ADR)-based chemotherapy. UCA1 silencing has enhanced the cytotoxic effect of this chemotherapeutic agent and suppressed the HIF-1α-associated glycolysis in ADR-resistant AML cells. Based on these results, UCA1 has been shown to exert a positive role in conquering the chemoresistance in pediatric AML patients (72).

Several lncRNAs such as NEF, HISLA, MEG3, PVT1, HOTTIP, SNHG3, LINC00689, H19, HOTAIR, GASL1, NRCP, LINRIS, and FILNC1 have been identified as predictive markers for OS or DFS of cancer patients. The potential of a number of lncRNAs including AWPPH, NEF, HAND2-AS1, lncRNA- RP11-555H23.1, and GASL1 as diagnostic markers in cancer patients has also been verified. These data suggest the importance of these lncRNAs in diverse aspects of cancer biology.

Taken together, regulation of cancer metabolism is a critical role of lncRNAs which has been shown by several in vitro investigations and a number of clinical studies. Thus, these transcripts are putative therapeutic targets in cancer. The importance of this function of lncRNAs is further highlighted by the eminent role of tumor microenvironment in the evolution of cancer and the ubiquitous dysregulation of metabolism in different cancer types. Thus, therapeutic targeting of these lncRNAs can be applied in diverse cancer types.

Statements

Author contributions

SG-F and MT wrote the draft and revised it. HS collected the required information and data. All authors contributed equally and fully aware of submission.

Funding

This study was financially supported by Shahid Beheshti University of Medical Sciences.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

References

  • 1.

    WarburgO. On the origin of cancer cells. Science. (1956) 123:309–14. 10.1126/science.123.3191.309

  • 2.

    DeberardinisRJChandelNS. Fundamentals of cancer metabolism. Sci Adv. (2016) 2:e1600200. 10.1126/sciadv.1600200

  • 3.

    Vander HeidenMGCantleyLCThompsonCB. Understanding the warburg effect: the metabolic requirements of cell proliferation. Science. (2009) 324:1029–33. 10.1126/science.1160809

  • 4.

    KoppenolWHBoundsPLDangCV. Otto Warburg's contributions to current concepts of cancer metabolism. Nat Rev Cancer. (2011) 11:325–37. 10.1038/nrc3038

  • 5.

    HanahanDWeinbergRA. Hallmarks of cancer: the next generation. Cell. (2011) 144:646–74. 10.1016/j.cell.2011.02.013

  • 6.

    BoroughsLKDeberardinisRJ. Metabolic pathways promoting cancer cell survival and growth. Nat Cell Biol. (2015) 17:351–9. 10.1038/ncb3124

  • 7.

    LinWZhouQWangC-QZhuLBiCZhangSet al. LncRNAs regulate metabolism in cancer. Int J Biol Sci. (2020) 16:1194–206. 10.7150/ijbs.40769

  • 8.

    IyerMKNiknafsYSMalikRSinghalUSahuAHosonoYet al. The landscape of long noncoding RNAs in the human transcriptome. Nat Genet. (2015) 47:199. 10.1038/ng.3192

  • 9.

    BatistaPJChangHY. Long noncoding RNAs: cellular address codes in development and disease. Cell. (2013) 152:1298–307. 10.1016/j.cell.2013.02.012

  • 10.

    ZhangXWangWZhuWDongJChengYYinZet al. Mechanisms and functions of long non-coding RNAs at multiple regulatory levels. Int J Mol Sci. (2019) 20:5573. 10.3390/ijms20225573

  • 11.

    LiH-JLiXPangHPanJ-JXieX-JChenW. Long non-coding RNA UCA1 promotes glutamine metabolism by targeting miR-16 in human bladder cancer. Jpn J Clin Oncol. (2015) 45:1055–63. 10.1093/jjco/hyv132

  • 12.

    RedisRSVelaLELuWDe OliveiraJFIvanCRodriguez-AguayoCet al. Allele-specific reprogramming of cancer metabolism by the long non-coding RNA CCAT2. Mol Cell. (2016) 61:520–34. 10.1016/j.molcel.2016.01.015

  • 13.

    MaMXuHLiuGWuJLiCWangXet al. Metabolism-induced tumor activator 1 (MITA1), an energy stress–inducible long noncoding RNA, promotes hepatocellular carcinoma metastasis. Hepatology. (2019) 70:215–30. 10.1002/hep.30602

  • 14.

    ZhouYHuangYHuKZhangZYangJWangZ. HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p. Cell Death Dis. (2020) 11:1–14. 10.1038/s41419-020-2366-7

  • 15.

    XuYLuoXHeWChenGLiYLiWet al. Long non-coding RNA PVT1/miR-150/HIG2 axis regulates the proliferation, invasion and the balance of iron metabolism of hepatocellular carcinoma. Cell Physiol Biochem. (2018) 49:1403–19. 10.1159/000493445

  • 16.

    SunKHuPXuF. LINC00152/miR-139-5p regulates gastric cancer cell aerobic glycolysis by targeting PRKAA1. Biomed Pharmacother. (2018) 97:1296–302. 10.1016/j.biopha.2017.11.015

  • 17.

    WuDZhaoBCaoXWanJ. Long non-coding RNA LINK-A promotes glioma cell growth and invasion via lactate dehydrogenase A. Oncol Rep. (2017) 38:1525–32. 10.3892/or.2017.5806

  • 18.

    HuMFuQJingCZhangXQinTPanY. LncRNA HOTAIR knockdown inhibits glycolysis by regulating miR-130a-3p/HIF1A in hepatocellular carcinoma under hypoxia. Biomed Pharmacother. (2020) 125:109703. 10.1016/j.biopha.2019.109703

  • 19.

    MaYHuMZhouLLingSLiYKongBet al. Long non-coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase-2. Oncol Lett. (2019) 18:2212–9. 10.3892/ol.2019.10551

  • 20.

    LiH-JSunX-MLiZ-KYinQ-WPangHPanJ-Jet al. LncRNA UCA1 promotes mitochondrial function of bladder cancer via the MiR-195/ARL2 signaling pathway. Cell Physio Biochem. (2017) 43:2548–61. 10.1159/000484507

  • 21.

    LiZLiXWuSXueMChenW. Long non-coding RNA UCA1 promotes glycolysis by upregulating hexokinase 2 through the mTOR–STAT3/microRNA143 pathway. Cancer Sci. (2014) 105:951–5. 10.1111/cas.12461

  • 22.

    MathupalaSKoYAPedersenPL. Hexokinase II: cancer's double-edged sword acting as both facilitator and gatekeeper of malignancy when bound to mitochondria. Oncogene. (2006) 25:4777–86. 10.1038/sj.onc.1209603

  • 23.

    ChenXWangLWangH. LINC01638 lncRNA promotes cancer cell proliferation in hepatocellular carcinoma by increasing cancer cell glucose uptake. Oncol Lett. (2019) 18:3811–6. 10.3892/ol.2019.10682

  • 24.

    StaffTPG. Correction: fMiRNA-192 and miRNA-204 directly suppress lncRNA HOTTIP and interrupt GLS1-mediated glutaminolysis in hepatocellular carcinoma. PLoS Genet. (2016) 12:e1005825. 10.1371/journal.pgen.1005825

  • 25.

    LiXZhaoQQiJWangWZhangDLiZet al. lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma. Int J Oncol. (2018) 53:551–66. 10.3892/ijo.2018.4418

  • 26.

    ChengZLuoCGuoZ. LncRNA-XIST/microRNA-126 sponge mediates cell proliferation and glucose metabolism through the IRS1/PI3K/Akt pathway in glioma. J Cell Biochem. (2019) 121:2170–83. 10.1002/jcb.29440

  • 27.

    HeZYouCZhaoD. Long non-coding RNA UCA1/miR-182/PFKFB2 axis modulates glioblastoma-associated stromal cells-mediated glycolysis and invasion of glioma cells. Biochem Biophys Res Commun. (2018) 500:569–76. 10.1016/j.bbrc.2018.04.091

  • 28.

    LiuXZhuQGuoYXiaoZHuLXuQ. LncRNA LINC00689 promotes the growth, metastasis and glycolysis of glioma cells by targeting miR-338-3p/PKM2 axis. Biomed Pharmacother. (2019) 117:109069. 10.1016/j.biopha.2019.109069

  • 29.

    XingZZhangYLiangKYanLXiangYLiCet al. Expression of long noncoding RNA YIYA promotes glycolysis in breast cancer. Cancer Res. (2018) 78:4524–32. 10.1158/0008-5472.CAN-17-0385

  • 30.

    ZhengXHanHLiuGPMaYXPanRLSangLJet al. LncRNA wires up hippo and hedgehog signaling to reprogramme glucose metabolism. EMBO J. (2017) 36:3325–35. 10.15252/embj.201797609

  • 31.

    ZhangLFuYGuoH. c-Myc-induced long non-coding RNA small nucleolar RNA host gene 7 regulates glycolysis in breast cancer. J Breast Cancer. (2019) 22:533–47. 10.4048/jbc.2019.22.e54

  • 32.

    MaFLiuXZhouSLiWLiuCChadwickMet al. Long non-coding RNA FGF13-AS1 inhibits glycolysis and stemness properties of breast cancer cells through FGF13-AS1/IGF2BPs/Myc feedback loop. Cancer Lett. (2019) 450:63–75. 10.1016/j.canlet.2019.02.008

  • 33.

    HanXYangYSunYQinLYangY. LncRNA TUG1 affects cell viability by regulating glycolysis in osteosarcoma cells. Gene. (2018) 674:87–92. 10.1016/j.gene.2018.06.085

  • 34.

    KangYZhuXXuYTangQHuangZZhaoZet al. Energy stress-induced lncRNA HAND2-AS1 represses HIF1α-mediated energy metabolism and inhibits osteosarcoma progression. Am J Cancer Res. (2018) 8:526.

  • 35.

    SongJWuXLiuFLiMSunYWangYet al. Long non-coding RNA PVT1 promotes glycolysis and tumor progression by regulating miR-497/HK2 axis in osteosarcoma. Biochem Biophys Res Commun. (2017) 490:217–24. 10.1016/j.bbrc.2017.06.024

  • 36.

    ZhangGMaAJinYPanGWangC. LncRNA SNHG16 induced by TFAP2A modulates glycolysis and proliferation of endometrial carcinoma through miR-490-3p/HK2 axis. Am J Transl Res. (2019) 11:7137.

  • 37.

    BaiJXuJZhaoJZhangR. Downregulation of lncRNA AWPPH inhibits colon cancer cell proliferation by downregulating GLUT-1. Oncol Lett. (2019) 18:2007–12. 10.3892/ol.2019.10515

  • 38.

    WangLCaoLWenCLiJYuGLiuC. LncRNA LINC00857 regulates lung adenocarcinoma progression, apoptosis and glycolysis by targeting miR-1179/SPAG5 axis. Human Cell. (2020) 33:195–204. 10.1007/s13577-019-00296-8

  • 39.

    YangBZhangLCaoYChenSCaoJWuDet al. Overexpression of lncRNA IGFBP4–1 reprograms energy metabolism to promote lung cancer progression. Mol Cancer. (2017) 16:154. 10.1186/s12943-017-0722-8

  • 40.

    WangXFaX-E. Knockdown of UCA1 inhibits viability and glycolysis by suppressing PKM2 expression through the mTOR pathway in non-small cell lung cancer cells. RSCAdv. (2018) 8:10610–9. 10.1039/C8RA00860D

  • 41.

    ShangCWangWLiaoYChenYLiuTDuQet al. LNMICC promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism. Cancer Res. (2018) 78:877–90. 10.1158/0008-5472.CAN-17-2356

  • 42.

    GaoWWengTWangLShiBMengWWangXet al. Long non-coding RNA NORAD promotes cell proliferation and glycolysis in non-small cell lung cancer by acting as a sponge for miR-136-5p. Mol Med Rep. (2019) 19:5397–405. 10.3892/mmr.2019.10210

  • 43.

    LangNWangCZhaoJShiFWuTCaoH. Long non-coding RNA BCYRN1 promotes glycolysis and tumor progression by regulating the miR-149/PKM2 axis in non-small-cell lung cancer. Mol Med Rep. (2020) 21:1509–16. 10.3892/mmr.2020.10944

  • 44.

    HuaQJinMMiBXuFLiTZhaoLet al. LINC01123, a c-Myc-activated long non-coding RNA, promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis. J Hematol Oncol. (2019) 12:1–18. 10.1186/s13045-019-0773-y

  • 45.

    GuSNiuXMaoFXuZ. Long noncoding RNA PCA3 regulates glycolysis, viability and apoptosis by mediating the miR-1/CDK4 axis in prostate cancer. RSC Adv. (2018) 8:37564–72. 10.1039/C8RA08083F

  • 46.

    ShaoMYuZZouJ. LncRNA-SNHG16 silencing inhibits prostate carcinoma cell growth, downregulate glut1 expression and reduce glucose uptake. Cancer Manag Res. (2020) 12:1751. 10.2147/CMAR.S231370

  • 47.

    HungC-LWangL-YYuY-LChenH-WSrivastavaSPetrovicsGet al. A long noncoding RNA connects c-Myc to tumor metabolism. Proc Natl Acad Sci USA. (2014) 111:18697–702. 10.1073/pnas.1415669112

  • 48.

    ChristensenLLTrueKHamiltonMPNielsenMMDamasNDDamgaardCKet al. SNHG16 is regulated by the Wnt pathway in colorectal cancer and affects genes involved in lipid metabolism. Mol Oncol. (2016) 10:1266–82. 10.1016/j.molonc.2016.06.003

  • 49.

    WangYLuJ-HWuQ-NJinYWangD-SChenY-Xet al. LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer. Mol Cancer. (2019) 18:1–18. 10.1186/s12943-019-1105-0

  • 50.

    TangJYanTBaoYShenCYuCZhuXet al. LncRNA GLCC1 promotes colorectal carcinogenesis and glucose metabolism by stabilizing c-Myc. Nat Commun. (2019) 10:1–15. 10.1038/s41467-019-11447-8

  • 51.

    CuiSYangXZhangLZhaoYYanW. LncRNA MAFG-AS1 promotes the progression of colorectal cancer by sponging miR-147b and activation of NDUFA4. Biochem Biophys Res Commun. (2018) 506:251–8. 10.1016/j.bbrc.2018.10.112

  • 52.

    SunSLiWMaXLuanH. Long noncoding RNA LINC00265 promotes glycolysis and lactate production of colorectal cancer through regulating of miR-216b-5p/TRIM44 axis. Digestion. (2019) 101:3911–400. 10.1159/000500195

  • 53.

    FanLHuangCLiJGaoTLinZYaoT. Long non-coding RNA urothelial cancer associated 1 regulates radioresistance via the hexokinase 2/glycolytic pathway in cervical cancer. Int J Mol Med. (2018) 42:2247–59. 10.3892/ijmm.2018.3778

  • 54.

    WuFZhouDCuiYShenGLiYWeiF. Long non-coding RNA UCA1 modulates the glycolysis of cervical cancer cells by miR-493-5p/HK2. Int J Clin Exp Pathol. (2018) 11:3943.

  • 55.

    ZhaoLJiGLeXWangCXuLFengMet al. Long noncoding RNA LINC00092 acts in cancer-associated fibroblasts to drive glycolysis and progression of ovarian cancer. Cancer Res. (2017) 77:1369–82. 10.1158/0008-5472.CAN-16-1615

  • 56.

    LiNZhanXZhanX. The lncRNA SNHG3 regulates energy metabolism of ovarian cancer by an analysis of mitochondrial proteomes. Gynecol Oncol. (2018) 150:343–54. 10.1016/j.ygyno.2018.06.013

  • 57.

    RupaimooleRLeeJHaemmerleMLingHPrevisRAPradeepSet al. Long noncoding RNA ceruloplasmin promotes cancer growth by altering glycolysis. Cell Rep. (2015) 13:2395–402. 10.1016/j.celrep.2015.11.047

  • 58.

    HeJLiFZhouYHouXLiuSLiXet al. LncRNA XLOC_006390 promotes pancreatic carcinogenesis and glutamate metabolism by stabilizing c-Myc. Cancer Lett. (2020) 469:419–28. 10.1016/j.canlet.2019.11.021

  • 59.

    SunJZhangPYinTZhangFWangW. Upregulation of LncRNA PVT1 facilitates pancreatic ductal adenocarcinoma cell progression and glycolysis by regulating MiR-519d-3p and HIF-1A. J Cancer. (2020) 11:2572. 10.7150/jca.37959

  • 60.

    LiuJLiuZ-XWuQ-NLuY-XWongC-WJuH-Qet al. IDDF2019-ABS-0200 Long non-coding RNA LOC148709 regulates PFKFB3-mediated glycolytic reprogramming in esophageal squamous cell carcinoma. BMJ. (2019) 68:1354–66. 10.1136/gutjnl-2019-IDDFAbstracts.37

  • 61.

    LiDChengMNiuYChiXLiuXFanJet al. Identification of a novel human long non-coding RNA that regulates hepatic lipid metabolism by inhibiting SREBP-1c. Int J Biol Sci. (2017) 13:349. 10.7150/ijbs.16635

  • 62.

    LiuGZhengXXuYLuJChenJHuangX. Long non-coding RNAs expression profile in HepG2 cells reveals the potential role of long non-coding RNAs in the cholesterol metabolism. Chinese Med J. (2015) 128:91. 10.4103/0366-6999.147824

  • 63.

    ZhaoYLiuYLinLHuangQHeWZhangSet al. The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1. Mol Cancer. (2018) 17:69. 10.1186/s12943-018-0820-2

  • 64.

    MoXWuYChenLZhaiMGaoZHuKet al. Global expression profiling of metabolic pathway-related lncRNAs in human gastric cancer and the identification of RP11-555H23. 1 as a new diagnostic biomarker. J Clin Lab Anal. (2019) 33:e22692. 10.1002/jcla.22692

  • 65.

    LuanWZhouZNiXXiaYWangJYanYet al. Long non-coding RNA H19 promotes glucose metabolism and cell growth in malignant melanoma via miR-106a-5p/E2F3 axis. J Cancer Res Clin Oncol. (2018) 144:531–42. 10.1007/s00432-018-2582-z

  • 66.

    YangXYeHHeMZhouXSunNGuoWet al. LncRNA PDIA3P interacts with c-Myc to regulate cell proliferation via induction of pentose phosphate pathway in multiple myeloma. Biochem Biophys Res Commun. (2018) 498:207–13. 10.1016/j.bbrc.2018.02.211

  • 67.

    ZouZWMaCMedoroLChenLWangBGuptaRet al. LncRNA ANRIL is up-regulated in nasopharyngeal carcinoma and promotes the cancer progression via increasing proliferation, reprograming cell glucose metabolism and inducing side-population stem-like cancer cells. Oncotarget. (2016) 7:61741. 10.18632/oncotarget.11437

  • 68.

    ZhaoCBaiXHuX. Knockdown of lncRNA XIST inhibits hypoxia-induced glycolysis, migration and invasion through regulating miR-381-3p/NEK5 axis in nasopharyngeal carcinoma. Eur Rev Med Pharmacol Sci. (2020) 24:2505–17. 10.26355/eurrev_202003_20518

  • 69.

    ZengBYeHChenJChengDCaiCChenGet al. LncRNA TUG1 sponges miR-145 to promote cancer progression and regulate glutamine metabolism via Sirt3/GDH axis. Oncotarget. (2017) 8:113650. 10.18632/oncotarget.21922

  • 70.

    ChuHLiZGanZYangZWuZRongM. LncRNA ELF3-AS1 is involved in the regulation of oral squamous cell carcinoma cell proliferation by reprogramming glucose metabolism. OncoTargets Ther. (2019) 12:6857. 10.2147/OTT.S217473

  • 71.

    WangYZhangXWangZHuQWuJLiYet al. LncRNA-p23154 promotes the invasion-metastasis potential of oral squamous cell carcinoma by regulating Glut1-mediated glycolysis. Cancer Lett. (2018) 434:172–83. 10.1016/j.canlet.2018.07.016

  • 72.

    ZhangYLiuYXuX. Knockdown of LncRNA-UCA1 suppresses chemoresistance of pediatric AML by inhibiting glycolysis through the microRNA-125a/hexokinase 2 pathway. J Cell Biochem. (2018) 119:6296–308. 10.1002/jcb.26899

  • 73.

    SunL-YLiX-JSunY-MHuangWFangKHanCet al. LncRNA ANRIL regulates AML development through modulating the glucose metabolism pathway of AdipoR1/AMPK/SIRT1. Mol Cancer. (2018) 17:127. 10.1186/s12943-018-0879-9

  • 74.

    WangY. Upregulated lncRNA-HNGA1, a target of miR-375, contributes to aerobic glycolysis of head and neck squamous cell carcinoma through increasing levels of the glucose transporter protein SCL2A1. Eur J Cancer. (2016) 61:S14–S5. 10.1016/S0959-8049(16)61039-0

  • 75.

    LiuXGanB. lncRNA NBR2 modulates cancer cell sensitivity to phenformin through GLUT1. Cell Cycle. (2016) 15:3471–81. 10.1080/15384101.2016.1249545

  • 76.

    ChangLXuWZhangYGongF. Long non-coding RNA-NEF targets glucose transportation to inhibit the proliferation of non-small-cell lung cancer cells. Oncol Letters. (2019) 17:2795–801. 10.3892/ol.2019.9919

  • 77.

    GongWYangLWangYXianJQiuFLiuLet al. Analysis of survival-related lncRNA landscape identifies a role for LINC01537 in energy metabolism and lung cancer progression. Int J Mol Sci. (2019) 20:3713. 10.3390/ijms20153713

  • 78.

    LiZLiuHJuWXingYZhangXYangJ. LncRNA GASL1 inhibits growth and promotes expression of apoptosis-associated proteins in prostate carcinoma cells through GLUT-1. Oncol Lett. (2019) 17:5327–34. 10.3892/ol.2019.10244

  • 79.

    ZhengY-LLiLJiaY-XZhangB-ZLiJ-CZhuY-Het al. LINC01554-mediated glucose metabolism reprogramming suppresses tumorigenicity in hepatocellular carcinoma via downregulating PKM2 expression and inhibiting Akt/mTOR signaling pathway. Theranostics. (2019) 9:796. 10.7150/thno.28992

  • 80.

    XiaoZ-DHanLLeeHZhuangLZhangYBaddourJet al. Energy stress-induced lncRNA FILNC1 represses c-Myc-mediated energy metabolism and inhibits renal tumor development. Nat Commun. (2017) 8:1–13. 10.1038/s41467-017-00902-z

  • 81.

    ChenSXuXLuSHuB. Long non-coding RNA HAND2-AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma. Oncol Lett. (2019) 18:1323–9. 10.3892/ol.2019.10445

  • 82.

    FanZWangXLiPMeiCZhangMZhaoC. Overexpression of lncRNA GATA6-AS inhibits cancer cell proliferation in mantle cell lymphoma by downregulating GLUT1. Oncol Lett. (2019) 18:2443–7. 10.3892/ol.2019.10540

  • 83.

    ShiZGuoFJiaDHuangJChenJSunMet al. Long non-coding RNA mortal obligate RNA transcript suppresses tumor cell proliferation in prostate carcinoma by inhibiting glucose uptake. Oncol Lett. (2019) 18:3787–91. 10.3892/ol.2019.10711

  • 84.

    HuRZhongPXiongLDuanL. Long noncoding RNA cancer susceptibility candidate 8 suppresses the proliferation of bladder cancer cells via regulating glycolysis. DNA Cell Biol. (2017) 36:767–74. 10.1089/dna.2017.3785

  • 85.

    MoXLiTXieYZhuLXiaoBLiaoQet al. Identification and functional annotation of metabolism-associated lnc RNA s and their related protein-coding genes in gastric cancer. Mol Genet Genom Med. (2018) 6:728–38. 10.1002/mgg3.427

  • 86.

    LiuXXiaoZ-DHanLZhangJLeeS-WWangWet al. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nat Cell Biol. (2016) 18:431–42. 10.1038/ncb3328

  • 87.

    LiaoMLiaoWXuNLiBLiuFZhangSet al. LncRNA EPB41L4A-AS1 regulates glycolysis and glutaminolysis by mediating nucleolar translocation of HDAC2. EBioMedicine. (2019) 41:200–13. 10.1016/j.ebiom.2019.01.035

  • 88.

    ZuoSWuLWangYYuanX. Long non-coding RNA MEG3 activated by vitamin d suppresses glycolysis in colorectal cancer via promoting c-myc degradation. Front Oncol. (2020) 10:274. 10.3389/fonc.2020.00274

  • 89.

    JonesRGThompsonCB. Tumor suppressors and cell metabolism: a recipe for cancer growth. Genes Dev. (2009) 23:537–48. 10.1101/gad.1756509

  • 90.

    De MatteisSRagusaAMarisiGDe DomenicoSCasadei GardiniABonafèMet al. Aberrant metabolism in hepatocellular carcinoma provides diagnostic and therapeutic opportunities. Oxidat Med Cell Longev. (2018) 2018:7512159. 10.1155/2018/7512159

  • 91.

    AdekolaKRosenSTShanmugamM. Glucose transporters in cancer metabolism. Curr Opin Oncol. (2012) 24:650–4. 10.1097/CCO.0b013e328356da72

  • 92.

    LiuHLuoJLuanSHeCLiZ. Long non-coding RNAs involved in cancer metabolic reprogramming. Cell Mol Life Sci. (2019) 76:495–504. 10.1007/s00018-018-2946-1

  • 93.

    JiangM-CNiJ-JCuiW-YWangB-YZhuoW. Emerging roles of lncRNA in cancer and therapeutic opportunities. Am J Cancer Res. (2019) 9:1354–66.

Summary

Keywords

lncRNA, cancer metabolism, expression, biomarker, oncogene

Citation

Ghafouri-Fard S, Shoorei H and Taheri M (2020) The Role of Long Non-coding RNAs in Cancer Metabolism: A Concise Review. Front. Oncol. 10:555825. doi: 10.3389/fonc.2020.555825

Received

26 April 2020

Accepted

24 August 2020

Published

06 October 2020

Volume

10 - 2020

Edited by

Xiao-Bin Lv, Third Affiliated Hospital of Nanchang University, China

Reviewed by

Krishna Beer Singh, University of Pittsburgh, United States; Qiyin Zhou, Zhejiang University, China; Kamla Kant Shukla, All India Institute of Medical Sciences Jodhpur, India

Updates

Copyright

*Correspondence: Mohammad Taheri

This article was submitted to Cancer Metabolism, a section of the journal Frontiers in Oncology

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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