AUTHOR=Fu Weilun , Wang Wenjing , Li Hao , Jiao Yuming , Weng Jiancong , Huo Ran , Yan Zihan , Wang Jie , Xu Hongyuan , Wang Shuo , Wang Jiangfei , Chen Dexi , Cao Yong , Zhao Jizong 

TITLE=CyTOF Analysis Reveals a Distinct Immunosuppressive Microenvironment in IDH Mutant Anaplastic Gliomas

JOURNAL=Frontiers in Oncology

VOLUME=Volume 10 - 2020

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.560211

DOI=10.3389/fonc.2020.560211

ISSN=2234-943X

ABSTRACT=The immune microenvironment is important for the development, progression, and prognosis of anaplastic glioma (AG). This complex milieu has not been fully elucidated, and single-cell analysis is urgently needed. Applying mass cytometry (CyTOF), we analyzed immunocytes from 5 patients with anaplastic astrocytoma, IDH-mutant (AAmut) and 10 patients with anaplastic oligodendroglioma, IDH-mutant and 1p/19q codeletion (AOD) and their matched peripheral blood mononuclear cells (PBMCs). Using a panel of 33 markers, we provide a single-cell view of the complex AG immune microenvironment. Our study confirmed that mononuclear phagocytes and T cells dominate the immune microenvironment of AGs. Glioma-associated microglia/macrophages in both AAmut and AOD showed highly immunosuppressive characteristics. Compared to those in the PBMCs, the proportions of exhausted CD4+ and CD8+ T cells were higher at the AG tumor sites. The immune microenvironment in AAmut exhibits more inhibitory characteristics than that in AOD.