AUTHOR=Zhang Lei , Ruan Yashi , Qin Zhiqiang , Gao Xian , Xu Kai , Shi Xiaokai , Gao Shenglin , Liu Shouyong , Zhu Kai , Wang Wei , Zuo Li , Zhang Lifeng , Zhang Wei 

TITLE=miR-483-3p, Mediated by KLF9, Functions as Tumor Suppressor in Testicular Seminoma via Targeting MMP9

JOURNAL=Frontiers in Oncology

VOLUME=Volume 10 - 2020

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.596574

DOI=10.3389/fonc.2020.596574

ISSN=2234-943X

ABSTRACT=Seminoma (SEM) is the most frequent testicular germ cell tumor with a high incidence in young men. Besides, many miRNAs have been identified as important regulators and biomarkers of testicular SEM. Nevertheless, the functional contributions of miR-483-3p to SEM remain unclear. Thus, the present study aims to explore the function and regulatory mechanism of miR-483-3p in SEM. miR-483-3p was down‐regulated in SEM tissues versus paracancerous normal tissues. The expression level of miR-483-3p was significantly associated with tumor stage by RT-qPCR. Functionally, miR-483-3p over-expression suppressed cell growth, migration and invasion in SEM cell lines. Mechanically, miR-483-3p negatively regulated MMP9 by directly binding to its 3’-UTR. The over-expression of miR-483-3p could reverse the promoting role of MMP9 over-expression on the proliferation, migration and invasion of TCam-2 cells. Moreover, KLF9 was an upstream regulator of miR-483-3p. In general, our study suggested that miR-483-3p could inhibit the cell growth, migration and invasion of testicular SEM by targeting MMP9. Moreover, KLF9 is an upstream positive regulator of miR-483-3p and also functions as a tumor suppressor in SEM.