AUTHOR=Su Jing , Huang Lynn S. , Barnard Ryan , Parks Graham , Cappellari James , Bellinger Christina , Dotson Travis , Craddock Lou , Prakash Bharat , Hovda Jonathan , Clark Hollins , Petty William Jeffrey , Pasche Boris , Chan Michael D. , Miller Lance D. , Ruiz Jimmy 

TITLE=Comprehensive and Computable Molecular Diagnostic Panel (C2Dx) From Small Volume Specimens for Precision Oncology: Molecular Subtyping of Non-Small Cell Lung Cancer From Fine Needle Aspirates

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.584896

DOI=10.3389/fonc.2021.584896

ISSN=2234-943X

ABSTRACT=<p>The <italic>Comprehensive, Computable NanoString Diagnostic gene panel</italic> (C2Dx) is a promising solution to address the need for a molecular pathological research and diagnostic tool for precision oncology utilizing small volume tumor specimens. We translate subtyping-related gene expression patterns of Non-Small Cell Lung Cancer (NSCLC) derived from public transcriptomic data which establish a highly robust and accurate subtyping system. The C2Dx demonstrates supreme performance on the NanoString platform using microgram-level FNA samples and has excellent portability to frozen tissues and RNA-Seq transcriptomic data. This workflow shows great potential for research and the clinical practice of cancer molecular diagnosis.</p>