AUTHOR=Chan Ren-Hao , Lin Peng-Chan , Chen Shang-Hung , Lin Shao-Chieh , Chen Po-Chuan , Lin Bo-Wen , Shen Meng-Ru , Yeh Yu-Min TITLE=Clinical Utility of a Cell-Free DNA Assay in Patients With Colorectal Cancer JOURNAL=Frontiers in Oncology VOLUME=Volume 11 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.589673 DOI=10.3389/fonc.2021.589673 ISSN=2234-943X ABSTRACT=
The analysis of cell-free DNA (cfDNA) is rapidly emerging as a powerful approach to guide the clinical care of cancer patients. Several comprehensive cfDNA assays designed to detect mutations across several genes are now available. Here, we analyzed the use of a cfDNA panel in colorectal cancer (CRC) patients. Twenty-eight CRC patients with relapse or metastatic disease and 31 patients with no evidence of disease (NED) were enrolled. Genomic alterations in cfDNA were analyzed by the Oncomine™ Pan-Cancer Cell-Free Assay that detects hotspot mutations, small indels, copy number changes, and gene fusions across 52 genes. In the NED group, genomic alterations in cfDNA were detected in 12/31 patients (38.7%). The detection of alterations was more common in patients who were ≥60 years old, and the most common genomic alteration was a