AUTHOR=Dong Xiaoxi , Zheng Tiantian , Zhang Minhua , Dai Chao , Wang Lili , Wang Lei , Zhang Ruipeng , Long Yuan , Wen Danyi , Xie Feng , Zhang Yue , Huang Yong , Dong Jianguo , Liu Huan , Du Pan , King Bonnie L. , Tan Winston , Jia Shidong , Lu Chris X. , Kohli Manish , Wang Haitao , Yu Jianjun 

TITLE=Circulating Cell-Free DNA-Based Detection of Tumor Suppressor Gene Copy Number Loss and Its Clinical Implication in Metastatic Prostate Cancer

JOURNAL=Frontiers in Oncology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.720727

DOI=10.3389/fonc.2021.720727

ISSN=2234-943X

ABSTRACT=<p>Current liquid biopsy assays lack sufficient sensitivity to detect copy number loss, which limits the interrogation of critical tumor suppressor gene deletions during cancer progression and treatment. Here we describe a liquid biopsy assay with improved sensitivity for detection of copy number loss in blood samples with low levels of circulating tumor DNA, and demonstrate its utility by profiling <italic>PTEN</italic>, <italic>RB1</italic>, and <italic>TP53</italic> genetic loss in metastatic prostate cancer patients.</p>