AUTHOR=Hayashi Kumiko , Nogawa Daichi , Kobayashi Maki , Asakawa Ayaka , Ohata Yae , Kitagawa Shota , Kubota Kazuishi , Takahashi Hisashi , Yamada Miyuki , Oda Goshi , Nakagawa Tsuyoshi , Uetake Hiroyuki , Onishi Iichiroh , Kinowaki Yuko , Kurata Morito , Kitagawa Masanobu , Yamamoto Kouhei 

TITLE=Quantitative high-throughput analysis of tumor infiltrating lymphocytes in breast cancer

JOURNAL=Frontiers in Oncology

VOLUME=Volume 12 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.901591

DOI=10.3389/fonc.2022.901591

ISSN=2234-943X

ABSTRACT=Detailed subsets of tumor infiltrating lymphocytes (TILs) were quantified from breast cancer (BC) tissues and compared among BC subtypes. The TILs of BC tissues from 86 patients were classified using multiplex immunohistochemistry and an artificial intelligence-based analysis system based on T-cell subset markers, immunomodification markers, and the localization of TILs. The levels of CD4/PD1 and CD8/PD1 double-positive stromal TILs were significantly lower in the HER2- BC subtype (p <0.01 and p <0.05, respectively). In triple-negative breast cancer (TNBC), single marker-positive intratumoral TILs did not affect prognosis, however CD4/PDL1, CD8/PD1, and CD8/PDL1 double-positive TILs were significantly associated with TNBC recurrence (p<0.05, p<0.01, and p<0.001, respectively).TIL profiles differed among different breast cancer subtypes, suggesting that the localization of TILs and their tumor-specific subsets influence the BC microenvironment.