AUTHOR=Li Linwei , Li Qiwei , Niu Rui , Sun Wei , Liang Hua 

TITLE=Single-cell RNA sequencing in diffuse large B-cell lymphoma: tumor heterogeneity, microenvironment, resistance, and prognostic markers

JOURNAL=Frontiers in Oncology

VOLUME=Volume 15 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2025.1583250

DOI=10.3389/fonc.2025.1583250

ISSN=2234-943X

ABSTRACT=Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous malignancy with challenges in treatment resistance and relapse. Single-cell RNA sequencing (scRNA-seq) has provided important insights into tumor heterogeneity, microenvironment interactions, resistance mechanisms, and prognostic biomarkers. This review summarizes key findings from scRNA-seq studies, which have deepened our understanding of DLBCL and contributed to the development of precision therapeutic strategies. Integrating scRNA-seq with spatial transcriptomics and single-cell multi-omics may further elucidate disease mechanisms and identify novel therapeutic targets, supporting the advancement of precision medicine in DLBCL.