AUTHOR=Liu Feng , Wu Xiaojun , Yu Shaoqing , Cheng Deqiang , Pan Jun , Wang Xiaodong , Yu Guanzhen , Li Chaofu 

TITLE=Spatial transcriptomic analysis of 4NQO-induced tongue cancer revealed cellular lineage diversity and evolutionary trajectory

JOURNAL=Frontiers in Oncology

VOLUME=Volume 15 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2025.1592044

DOI=10.3389/fonc.2025.1592044

ISSN=2234-943X

ABSTRACT=IntroductionSpatial transcriptomic analysis has proposed valuable insights into the behavior of tongue cancer. However, the specific cell types involved in chemically-induced carcinogenesis and the process of tumor development remain elusive.MethodsWe leveraged artificial intelligence (AI) algorithms and spatial transcriptomic sequencing to meticulously characterize the spatial and temporal evolution of 4-nitroquinoline-1-oxide (4NQO)-induced tongue carcinogenesis and intratumor heterogeneity.ResultsAn AI classifier effectively categorized dysplastic tongue tissue into 13 distinct groups. Spatial transcriptomics identified 13 corresponding cellular subgroups with unique features within the lesion. Both methods successfully distinguished subtle muscle phenotype and genetic lineage variations induced by 4NQO, despite limited morphological differences. Evolutionary tree analysis revealed the dynamic appearance and disappearance of functionally and genetically diverse cell subgroups during the progression from epithelial dysplasia to in situ carcinoma and invasive cancer. Key findings include the identification of specific switch genes associated with tumor invasion and the revelation of significant intratumor heterogeneity.DiscussionThis spatial transcriptomic analysis of 4NQO-induced tongue cancer provides a detailed characterization of tumor evolution and heterogeneity. It elucidates critical aspects of tongue cancer cell behavior and identifies potential therapeutic targets (switch genes). These findings offer novel insights for improving the diagnosis and treatment of tongue cancer.