AUTHOR=Laliberté Catherine , Ng Nicole , Eymael Denise , Higgins Kevin , Ali Aiman , Kiss Alex , Bradley Grace , Magalhaes Marco A. O. 

TITLE=Characterization of Oral Squamous Cell Carcinoma Associated Inflammation: A Pilot Study

JOURNAL=Frontiers in Oral Health

VOLUME=Volume 2 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2021.740469

DOI=10.3389/froh.2021.740469

ISSN=2673-4842

ABSTRACT=Background: Oral squamous cell carcinoma (OSCC) is a devastating disease that is usually associated with a dense associated inflammatory infiltrate. Characterizing tumour-associated inflammation is critical to understand the pathogenies of tumour development and progression Methods: We have tested a protocol to analyze tissue and salivary immune cells and mediators of 37 patients with OSCC at different stages and compared to 8 chronic periodontitis patients and 24 healthy controls.  Tissue analysis was based on fluorescent immunohistochemistry and inflammatory mediators were analyzed using a Luminex-based 30-Plex panel. Immune cells were analyzed using multichannel flow cytometry including CD45, CD66b, CD3, CD4, CD8, CD25, CD56, CD68, CD138, PD-1 and PD-L1. Results: We show an increase in OSCC-associated inflammation characterized by increased pro-inflammatory cytokines including IL-6, IL-8, TNF, and GMCSF and increased salivary immune cells. Conclusion: We described a new method to analyze salivary inflammatory markers that can be used in future studies to monitor disease progression and prognosis