AUTHOR=Diallo Mamadou Alpha , Ndiaye Ibrahima M. , Sow Djiby , Seck Mame Cheikh , Diongue Khadim , Touré Mariama , Battle Katerine E. , Ngom Bassirou , Sy Mouhamad , Gaye Amy , Ndiaye Yaye Dié , Garba Mamane Nassirou , Badiane Aida Sadikh , Sene Aita , Ndiop Medoune , Gomis Jules François , Volkman Sarah K. , Sene Doudou , MacInnis Bronwyn L. , Diallo Ibrahima , Ndiaye Mouhamadou , Wirth Dyann F. , Ndiaye Daouda 

TITLE=RDT performance through high-throughput bead-based antigen detection during malaria school survey in Senegal

JOURNAL=Frontiers in Parasitology

VOLUME=Volume 4 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/parasitology/articles/10.3389/fpara.2025.1598280

DOI=10.3389/fpara.2025.1598280

ISSN=2813-2424

ABSTRACT=BackgroundRapid Diagnostic Tests (RDTs) remain the frontline tool for malaria diagnosis, but their performance in detecting low-density infections is variable and poorly characterized at the population level.ObjectiveThis study aimed to evaluate the diagnostic performance of HRP2-based RDTs by integrating high-throughput bead-based HRP2 quantification into school-based malaria surveys.MethodsA cross-sectional study was conducted in three Senegalese districts (Diourbel, Tambacounda, and Kédougou), enrolling 3,748 school-aged children. All participants were tested using RDTs, and dried blood spots were analyzed with a multiplex bead-based HRP2 assay. A Gaussian mixture model was used to classify HRP2 positivity, and logistic regression assessed the relationship between HRP2 concentration and RDT outcome.ResultsThe overall RDT positivity rate was 7.2%, with marked heterogeneity across districts (Diourbel: 3.0%, Kédougou: 15.9%, Tambacounda: 7.6%). HRP2 concentration was the strongest predictor of RDT positivity (aOR: 14.55 per log10 increase, 95% CI: 11.14–19.00). RDT limits of detection (LOD95) varied significantly: 3.9 ng/mL in Tambacounda, 121.2 ng/mL in Kédougou, and 204.3 ng/mL in Diourbel.ConclusionRDTs remain a useful surveillance tool, particularly in moderate- to high-transmission settings. However, reduced sensitivity at lower antigen concentrations in hypo-endemic areas highlights the value of complementary high-sensitivity assays for elimination-focused strategies. Future research should explore the application of these integrated diagnostic approaches in regions without seasonal malaria chemoprophylaxis intervention.