AUTHOR=Sagheddu Valeria , Patrone Vania , Miragoli Francesco , Puglisi Edoardo , Morelli Lorenzo 

TITLE=Infant Early Gut Colonization by Lachnospiraceae: High Frequency of Ruminococcus gnavus

JOURNAL=Frontiers in Pediatrics

VOLUME=Volume 4 - 2016

YEAR=2016

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2016.00057

DOI=10.3389/fped.2016.00057

ISSN=2296-2360

ABSTRACT=<p><italic>Lachnospiraceae</italic> is a bacterial family usually isolated from human and mammalian intestinal microbiota. However, its presence and role in the infant microbiota is not fully elucidated. This may be due to the strictly anaerobic behavior of its members that hampers the possibility of culture-dependent enumeration. Here, we report on the presence of this bacterial group, using biomolecular techniques, in stool samples from 25 babies aged between 1 and 24 months. Denaturing gradient gel electrophoresis (DGGE) was used as a first detection step, and data were confirmed by quantitative PCR (qPCR). The DGGE showed the presence of <italic>Lachnospiraceae</italic> in infant fecal specimens and indicated the prevalence of <italic>Ruminococcus gnavus</italic> (<italic>R. gnavus</italic>). The qPCR confirmed the presence of the <italic>Clostridium XVIa</italic> group, <italic>Blautia</italic> genus, and <italic>R. gnavus</italic>, which are the main members of this family. We detected <italic>R. gnavus</italic> in 22 of 25 (88%) samples with a qPCR probe assay. Despite the difficulties associated with their detection and enumeration, <italic>Lachnospiraceae</italic>, and in particular <italic>R. gnavus</italic>, should be included in future studies on the infant microbiota composition.</p>