AUTHOR=Yao Meiqing , He Qin , Yang Manqiong , Wu Zhixiang , Li Ying , Kong Min , Kang Zhijuan , Yi Lu , Hu Yanan , Huang Lihua , Li Zhuoying , Yang Zuocheng 

TITLE=Association of miR-181c/d gene locus rs8108402 C/T polymorphism with susceptibility to Kawasaki disease in Chinese children

JOURNAL=Frontiers in Pediatrics

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2022.899779

DOI=10.3389/fped.2022.899779

ISSN=2296-2360

ABSTRACT=ABSTRACT
Background: Kawasaki disease (KD) is an acute systemic vasculitis of unknown etiology. The rs8108402 C/T single nucleotide polymorphism (SNP) is located in the promoter region of miR-181-c/d and the intron of Nanos 3. The miR-181 family contributes to the pathogenesis of cardiovascular and inflammatory disorders, while Nanos 3 is involved in DNA transcription regulation and cell proliferation. However, no studies have examined the association between miR-181c/d and Nanos 3 polymorphisms and the susceptibility and development of KD.
Objective: The purpose of our study was to examine the association of miR-181c/miR-181d/Nanos 3 gene locus rs8108402 C/T polymorphism with KD and the development of coronary artery lesions (CAL).
Methods: Peripheral blood specimens from 100 children with KD and 100 healthy children were collected. The locus rs8108402 C/T of miR-181c/d gene polymorphism was detected using polymerase chain reaction-sequencing-based typing technique.
Results: There were statistically significant differences in C and T allele frequency distributions between the KD group and healthy controls for the polymorphic site rs8108402 C/T (P=0.002). Moreover, the distribution of the genotypes CC, CT, and TT also showed statistical difference between the KD and control groups (P=0.003). Compared to the rs8108402 C allele, the T allele was significantly associated with increased KD susceptibility (OR=2.080, 95% CI=1.317~3.283). However, there were no significant associations discovered between the rs8108402 C/T polymorphism and CAL formation risk in the KD group.
Conclusion: SNP rs8108402 C/T located in the miR-181c/d promoter and Nanos3 intronic region is associated with susceptibility to Kawasaki disease in Chinese children, but not associated with the development of coronary artery lesions in the same population.

Keywords: Kawasaki disease; miR-181; Nanos3; coronary artery lesions; single-nucleotide polymorphisms