AUTHOR=Haddad Kashani Hamed , Seyed Hosseini Elahe , Nikzad Hossein , Soleimani Alireza , Soleimani Maryam , Tamadon Mohammad Reza , Keneshlou Fariba , Asemi Zatollah 

TITLE=The Effects of Vitamin D Supplementation on Signaling Pathway of Inflammation and Oxidative Stress in Diabetic Hemodialysis: A Randomized, Double-Blind, Placebo-Controlled Trial

JOURNAL=Frontiers in Pharmacology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00050

DOI=10.3389/fphar.2018.00050

ISSN=1663-9812

ABSTRACT=<p><bold>Objective:</bold> This study was carried out to determine the effects of vitamin D supplementation on signaling pathway of inflammation and oxidative stress in diabetic hemodialysis (HD) patients.</p><p><bold>Methods:</bold> This randomized double-blind placebo-controlled clinical trial was conducted among 60 diabetic HD patients. Subjects were randomly allocated into two groups to intake either vitamin D supplements at a dosage of 50,000 IU (<italic>n</italic> = 30) or placebo (<italic>n</italic> = 30) every 2 weeks for 12 weeks. Gene expression of inflammatory cytokines and biomarkers of oxidative stress were assessed in peripheral blood mononuclear cells (PBMCs) of diabetic HD patients with RT-PCR method.</p><p><bold>Results:</bold> Results of RT-PCR indicated that after the 12-week intervention, compared to the placebo, vitamin D supplementation downregulated gene expression of interleukin (IL)-1β (<italic>P</italic> = 0.02), tumor necrosis factor alpha (TNF-α) (<italic>P</italic> = 0.02) and interferon gamma (IFN-γ) (<italic>P</italic> = 0.03) in PBMCs of diabetic HD patients. Additionally, vitamin D supplementation, compared to the placebo, downregulated gene expression of transforming growth factor beta (TGF-β) (<italic>P</italic> = 0.04), protein kinase C (PKC) (<italic>P</italic> = 0.001), and mitogen-activated protein kinases 1 (MAPK1) (<italic>P</italic> = 0.02) in PBMCs of diabetic HD patients. Although not significant, vitamin D supplementation let to a reduction of nuclear factor kappa B (NF-kB) (<italic>p</italic> = 0.75) expression in PBMCs isolated from diabetic patients compared to the placebo group. There was no statistically significant change following supplementation with vitamin D on gene expression of interleukin (IL)-4, IL-6, and vascular endothelial growth factor (VEGF) in PBMCs of diabetic HD patients.</p><p><bold>Conclusions:</bold> Overall, we found that vitamin D supplementation for 12 weeks among diabetic HD patients had beneficial effects on few gene expression related to inflammation and oxidative stress.</p><p><bold>Clinical trial registration:</bold> IRCT201701035623N101. Registered on January 8, 2017.</p>