AUTHOR=Huang Boxian , Ding Chenyue , Zou Qinyan , Lu Jiafeng , Wang Wei , Li Hong 

TITLE=Human Amniotic Fluid Mesenchymal Stem Cells Improve Ovarian Function During Physiological Aging by Resisting DNA Damage

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00272

DOI=10.3389/fphar.2020.00272

ISSN=1663-9812

ABSTRACT=Many studies show that mesenchymal stem cells have the capability to restore the function of premature ovarian insufficiency disease, few studies are relative to the treatment of ovarian physiologic aging (OPA) by the stem cell. We designed this experimental study to find out whether human amniotic fluid mesenchymal stem cells (hAFMSCs) provide the ability to recover vitality of ovary and how to make them work in this process. The 12-14 months mice were used in this study, and young fertile female mice (3-5 months old) were the control group. The ovary markers at four stage and DNA damage genes were tested by qPCR and western blot. After the hAFMSCs were used to cure the OPA mice model, the hAFMSCs displayed better therapeutic activity on the function of mouse ovary, increasing the follicle numbers and improving the hormone level. Besides, our results demonstrated that hAFMSCs elevated the marker expression level of ovarian granular cells significantly that were from OPA patients. In the meantime, we found thatthe proliferation activity was improved and the apoptosis level was inhibited more powerfully after hAFMSCs co-cultured with OPA hGCs. Finally, in this study, we found that hAFMSCs increased the gene and protein expression of ovary markers at four stage and inhibited the expression level of DNA damage genes. These works give insight into the view that hAFMSCs play an integral role in resistance to OPA. Furthermore, our present study demonstrated that hAMSCs recovers ovary function in OPA through restoring the expression of DNA damage genes.