AUTHOR=Khalil Ayman , Al-Massarani Ghassan , Aljapawe Abdulmunim , Ekhtiar Adnan , Bakir M. Adel 

TITLE=Resveratrol Modulates the Inflammatory Profile of Immune Responses and Circulating Endothelial Cells’ (CECs’) Population During Acute Whole Body Gamma Irradiation

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.528400

DOI=10.3389/fphar.2020.528400

ISSN=1663-9812

ABSTRACT=Wistar rats were whole body irradiated with a single dose of 2Gy (WBI) in presence of 10 or 100 mg/kg of resveratrol (RSV) for 30 days prior to irradiation. Livers were extracted and flow cytometry assay was conducted for detection of 13 profiles of immune response Th1, Th2, Th9, Th17 and Th22. In addition, peripheral blood were collected and analyzed for circulating endothelial cells (CECs) as markers for endothelial damage. Results demonstrated that resveratrol enhances the immunological response of livers towards irradiation via the induction of T-helper 2 (Th2) - immune response mainly via the ilterleukine-10 (IL-10) secretion with more than 5000 picMol/ml in presence of 100 mg/kg/bw and 2Gy of WBI. Also, the results indicated that RSV decreases at 100 mg/kg/bw dose the main pro-inflammatory cytokines such as INF-, IL-22, IL-17A and GM-CSF post irradiation exposure. In addition, mRNA of IL-10 and its protein levels from the isolated liver kupffer cells beside to the up regulation of number of these cells at RSV 100 mg post irradiation demonstrated that kupffer cells of the liver were the central source of this anti-inflammatory response mediated mainly by IL10. These results proposed for the first time that RSV promotes IL-10 mediated immune resolution by kupffer cells and not by hepatocytes that makes KCs has a special and very important role in radiotherapy. In addition, RSV has anti-apoptotic priorities through re-increasing the number of circulate CECs, which implicated in irradiation damage. Results of current work disclose novel findings about the potential of RSV as a radio-protector of natural origin, and suggest novel roles of KCs as pharmacological target during radiation exposure.