AUTHOR=Guo Yan , Fan Wenxue , Xie Yuefeng , Cao Shuyu , Wan Haitong , Jin Bo 

TITLE=SIRT1 Is the Target Gene for 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-Glucoside Alleviating the HUVEC Senescence

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 11 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.542902

DOI=10.3389/fphar.2020.542902

ISSN=1663-9812

ABSTRACT=This study aimed to explore the effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG) on the senescence of human umbilical vein cells (HUVECs) induced by hydrogen peroxide (H2O2) and to identify the potential targets mediating its protective action. HUVEC cells pre-treated with TSG for 24h were exposed to H2O2 treatment. TSG significantly decreased H2O2-induced cellular senescence, as indicated by reduced senescence-associated β-galactosidase (SA-β-gal) positive staining, the proportion of cells in the G1 phase, cell apoptosis, p21, and plasminogen activator inhibitor-1 (PAI-1) expression. Moreover, TSG promoted SIRT1 expression. When SIRT1 was inhibited by EX527 or SIRT1 siRNA, the anti-aging effect of TSG is diminished according to the increased proportion of cells in the G1 phase, cell apoptosis, p21, and PAI-1 expression. Overall, our study established TSG as an anti-senescence compound that exerts its protective action by regulating SIRT1 expression.