AUTHOR=Yu Ping , Li Yuangeng , Fu Wenwen , Li Xin , Liu Yanzhe , Wang Yaozhen , Yu Xiaofeng , Xu Huali , Sui Dayun 

TITLE=Panax quinquefolius L. Saponins Protect Myocardial Ischemia Reperfusion No-Reflow Through Inhibiting the Activation of NLRP3 Inflammasome via TLR4/MyD88/NF-κB Signaling Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 11 - 2020

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.607813

DOI=10.3389/fphar.2020.607813

ISSN=1663-9812

ABSTRACT=At present, many patients who undergo reperfusion immediately after percutaneous coronary intervention will occur microvascular obstruction and reduce myocardial blood flow. This phenomenon is called "no-reflow (NR)", and there is still no effective therapy for NR. Studies showed panax quinquefolius L. saponins (PQS) have effect on MI/R injury, while the effect and mechanism of PQS on MI/R induced NR are not clear. In this study, we established a MI/R model to investigate whether PQS decrease NR phenomenon via suppression of inflammation. We found that PQS significantly alleviated the symptoms of NR by reducing ischemia, infarction and NR area, improving cardiac function, preventing pathological morphology changes of myocardium, depressing leukocytes’ aggregation and adhesion, suppressing the excessive inflammation. Further study demonstrated that PQS remarkably inhibited TLR4, MyD88, p-NF-κB, NLRP3 inflammasome-associated protein, and these effect could be reversed by LPS. These results indicated that PQS protect NR by inhibiting the activation of NLRP3 inflammasome via TLR4/MyD88/NF-κB signaling pathway, suggesting that PQS exist potential in preventing NR induced by MI/R.