AUTHOR=Yang Meng-Die , Zhou Wen-Jun , Chen Xiao-Le , Chen Jian , Ji Qing , Li Qi , Wang Wen-Hai , Su Shi-Bing 

TITLE=Therapeutic Effect and Mechanism of Bushen-Jianpi-Jiedu Decoction Combined with Chemotherapeutic Drugs on Postoperative Colorectal Cancer

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.524663

DOI=10.3389/fphar.2021.524663

ISSN=1663-9812

ABSTRACT=There is a lack of effective therapeutic drugs in patients with postoperative colorectal cancer (PCRC). This study was aim to investigate the therapeutic effect and mechanism of Bushen-Jianpi-Jiedu Decoction combined with chemotherapy on PCRC with liver and kidney yin deficiency and spleen deficiency syndrome (LKYD-SDS) by made analysis of clinical therapy data, integrative analysis of RNA-seq and Label-free data, analysis of network pharmacology and in vitro pharmacological experimental verification. In the assessment of clinical therapy, the median progression-free survival (PFS) and Karnofsky performance score (KPS) were increased in PCRC patients by BSJPJDD combined with chemotherapy treatment for 3 months, compared to chemotherapy alone (P<0.05). The analysis of RNA-seq and Label-free combined with network pharmacology showed that 559 DEGs and 11 DEPs were potential targets, which involved in the regulation of multiple signaling pathways, such as MAPK, PI3K-Akt and HIF-1 signaling pathway. In the experimental verification, ELISA assay showed that plasma ZEB2, CAT and KRT78 were decreased, IL-1Α, CD5L, FBLN5, EGF and KRT78 were increased by above comparison (P<0.05). Furthermore, the cell viability was inhibited by BSJPJDD combined with oxaliplatin and the expressions of p-ERK1/2 and p-ERK1/2 and ERK1/2 ratio were up-regulated in SW620 cells compared to BSJPJDD or oxaliplatin alone (P<0.001). These data suggested that BSJPJDD combined with chemotherapy prolongs the survival and improves quality of life of PCRC patients with LKYD-SDS, and may be associated with the regulation of MAPK/ERK signaling pathway.