AUTHOR=Zheng Tao , Yang Jun , Zhang Jing , Yang Chaojun , Fan Zhixing , Li Qi , Zhai Yuhong , Liu Haiyin , Yang Jian 

TITLE=Downregulated MicroRNA-327 Attenuates Oxidative Stress–Mediated Myocardial Ischemia Reperfusion Injury Through Regulating the FGF10/Akt/Nrf2 Signaling Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.669146

DOI=10.3389/fphar.2021.669146

ISSN=1663-9812

ABSTRACT=Although miR-327 put protective effect on cardiomyocytes as described previously, the potential mechanism still needs further exploration. The aim of this study is to investigate the role and mechanism of miR-327 on oxidative stress in myocardial ischemia/reperfusion injury(MI/RI) process. Oxidative stress and cardiomyocytes injury were detected in rats model of MI/RI, the hypoxia/reoxygenation (H/R) and tert-Butyl hydroperoxide(TBHP) model of H9c2 cells. In vitro, down-regulation of miR-327 inhibited both H/R and TBHP induced oxidative stress, ancd suppressed apoptosis. Meanwhile, fibroblast growth factor 10(FGF10) was enhanced by miR-327 knocked down, followed with activation of PI3K, p-Akt and the translocation of Nrf2. Whereas, miR-327 overexpression performed with an opposite effects. Consistent with the results in vitro, down-regulation of miR-327 attenuated reactive oxygen species(ROS) generation and I/R injury. In a conclusion, inhibition of miR-327 could improve antioxidative ability and myocardial cell survival via regulating FGF10/Akt/Nrf2 pathway.