AUTHOR=Zhou Mingyue , Huo Jinhai , Wang Cairen , Wang Weiming 

TITLE=UPLC/Q-TOF MS Screening and Identification of Antibacterial Compounds in Forsythia suspensa (Thunb.) Vahl Leaves

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 12 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.704260

DOI=10.3389/fphar.2021.704260

ISSN=1663-9812

ABSTRACT=<p><italic>Forsythia suspensa</italic> (Thunb.) Vahl (<italic>F. suspensa</italic>) is a traditional Chinese medical herb and only its fruit is currently used in clinical therapies. However, the discarded parts like leaves also contain a large number of active components. In this study, we used macroporous adsorption resin to enrich the effective components from <italic>F. suspensa</italic> leaves. The separated active compounds were then identified and quantified by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC/Q-TOF MS) and high-performance liquid chromatography Active components with antibacterial properties extracted from <italic>F. suspensa</italic> leaves were confirmed <italic>in vitro</italic> and the corresponding mechanisms were explored. In sum, a stable and effective method for extracting antibacterial active components from <italic>F. suspensa</italic> leaves was established in this study, which proved the practicability of <italic>F. suspensa</italic> leaves as traditional Chinese medicine and is conducive to the more comprehensive utilization of the plant.</p>