AUTHOR=Shen Lei , Zong Wei , Feng Wei , Chen Erlin , Ma Shuo , Yuan Jie , Wang Guihua , Gu Xinliang , Shen Xianjuan , Ju Shaoqing 

TITLE=Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.840391

DOI=10.3389/fphar.2022.840391

ISSN=1663-9812

ABSTRACT=Objectives: Colorectal cancer (CRC) is a common carcinoma of gastrointestinal tract with high incidence and mortality worldwide. Studies have shown that long non-coding RNAs (lncRNAs) play important roles in CRC. Our purpose is to investigate the potential of serum Linc01836 as a diagnostic and prognostic marker in CRC.
Methods: We evaluated the expression of Linc01836 via quantitative real-time polymerase chain reaction (qRT-PCR). The serum CEA, CA19-9, Cyfra21-1 and CA72-4 concentrations were measured by Architect I4000 SR. Receiver operating characteristic (ROC) curves were plotted to estimate the diagnostic value in CRC. Relationship between serum Linc01836 expression and clinicopathological characteristics of CRC cases was analysed via chi-square test. The underlying mechanism of Linc01836 on the development and prognosis in CRC was predicted by bioinformatic analysis.
Results: The method of qRT-PCR for Linc01836 detection was confirmed with high precision and specificity. Serum Linc01836 expression in CRC patients was significantly higher than that in healthy donors (p<0.0001) and benign patients (p<0.0001), and declined after resection (p<0.01). High expression of Linc01836 was associated with histological stage (p=0.002) and lymph node metastasis (p=0.006). In addition, serum Linc01836 could effectively differentiate CRC patients from the healthy folks, with favorable area under the curve (AUC) of 0.809 (95% CI: 0.757-0.861, p<0.001). What’s more, the combination of serum Linc01836, CEA and Cyfra21-1 could improve diagnostic sensitivity (92.0%). Linc01836 was averagely located in nuclear and cytoplasm, suggesting that it might participate in CRC progression and prognosis through the crosstalk among lncRNAs, miRNAs and mRNAs.
Conclusions: Linc01836 may serve as a valuable non-invasive biomarker for population screening, early detection and clinical surveillance of CRC.