AUTHOR=Xu Duoduo , Liu Jia , Zheng Wei , Gao Qipin , Gao Yang , Leng Xiangyang 

TITLE=Identification of Polysaccharides From Dipsacus asperoides and Their Effects on Osteoblast Proliferation and Differentiation in a High-Glucose Environment

JOURNAL=Frontiers in Pharmacology

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.851956

DOI=10.3389/fphar.2022.851956

ISSN=1663-9812

ABSTRACT=Polysaccharides (DAI-1and DAI-2) from Dipsacus asperoides were obtained using mixed ion-exchange resin and Sephadex G-50 column chromatography then their properties, structures and activities were studied. The results demonstrated that DAI-1 and DAI-2 were homogeneous polysaccharides with molecular weights of 17kDa and 4kDa, respectively, that contained only glucose (Glc) residues. Results of methylation-based structural studies showed that backbones of DAI-1 and DAI-2 were composed of (1→6)-linked Glc residues. DAI-1 possessed a small number of side chains and a branch point of (1→3,6)- Glc, while DAI-2 lacked branching. Activity tests indicated that exposure of osteoblasts in a high-glucose environment to concentrations of DAI-1 of 25, 50 or 100 mg/ml induced cell proliferation and led to increased mRNA- and protein-level expression of bone morphogenetic protein 2 (BMP-2) and significantly promoted runt-related transcription factor 2 (Runx 2) expression. Moreover, DAI-1 treatment significantly increased alkaline phosphatase (ALP) and osteocalcin (OCN) activities in osteoblasts, suggesting that DAI-1 may exert activities that promote osteoblast proliferation and differentiation in a high-glucose environment.